Rui ZHANG Jiang QIAN Yifei YUAN
  • Department of Ophthalmology, Eye and ENT Hospital affiliated to Fudan University, Shanghai 200031, China Department of Ophthalmology, Eye and ENT Hospital affiliated to Fudan University, Shanghai 200031, China Department of Ophthalmology, Eye and ENT Hospital affiliated to Fudan University, Shanghai 200031, China;
Uveitis/immunology; Retinitis/ immunology; Tumor necrosis factoralpha/ administration & dosage; Antibodies, monoclonal/ administration & dosage; Animal experimentation
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective To observe the efficacy of the anti-tumor necrosis factor- alpha; monoclonal antibody (TNF- alpha; MCAb) in the treatment of experimental autoimmune uveoretinitis (EAU). Methods EAU animal models were induced by interphotoreceptor retinoid-binding protein (IRBP) R16 peptide with immunization. The rats were divided into 2 groups according to the injection times. TNF- alpha; MCAb was administered intravenously on day 6 or 4, 6 and 8 post-immunization respectively, and then to observe the clinical expression by slit-lamp microscope. Meanwhile, take the rats which did not accept TNF- alpha; MCAb as control group. Delayed type hypersensitivity (DTH) responses were measured on day 13 post-immunization of IRBP R16; the rats were killed on day 14 post-immunization of IRBP R16, and then enucleated the eyes for histopathological examination. To detect the cytokine level of IFN- gamma;, IL-4 in serum and IFN- gamma; in aqueous humor by enzyme-liked immunosorbent assay (ELISA) on day 14 post-injection. The hyperplasia responses of antigen specific lymphocyte of draining lymph node cells were detected. Results The TNF- alpha; MCAb group had mitigated ocular inflammation and decreased pathological grades compared with the control group; the IFN- gamma; concentrations in aqueous humor and serum were decreased, IL-4 was increased in serum; DTH responses were decreased; the hyperplasia responses of draining lymphocytes to IRBP R16 peptide were decreased, all the differences were statistically significant (P＜0.01). The rats accepted TNF- alpha; MCAb thrice had much better curative effect than the rats injected once (P＜0.05). Conclusions Injection of TNF- alpha; MCAb can inhibit ocular inflammation and specific immune cells of EAU remarkably and change the Th1/Th2 balance. Many times injections of TNF- alpha; MCAb were more effective than once.

Citation： Rui ZHANG Jiang QIAN Yifei YUAN. Treatment of experimental autoimmune uveoretinitis with anti-tumor necrosis factor-α monoclonal antibody. Chinese Journal of Ocular Fundus Diseases, 2008, 24(5): 336-339. doi: Copy