Huiying WANG Wei XU Yunli NIU Zhijian JIANG Ao RONG
  • Department of Ophthalmology, Tongji Hospital of Tongji University, Shanghai 200065,China Department of Ophthalmology, Tongji Hospital of Tongji University, Shanghai 200065,China Department of Ophthalmology, Tongji Hospital of Tongji University, Shanghai 200065,China Department of Ophthalmology, Tongji Hospital of Tongji University, Shanghai 200065,China;
Retinal neovascularization/ therapy; Gene therapy; Animal experimentation
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Objective To invesitgate the influence of recombinant adenovirus vector of human pigment epithelium-derived factor（AV-hPEDF）on retinal new vessels mediated by recombinant adenovirus vector. Methods Twenty 7-days-old Sprague-Dawley (SD) rat were divided into two groups randomly after the establishment of retinal neovascularization model. At postnatal 14 day, they were accepted intravitreal injection with blankadenovirus-vector (AV-Blank group) and adenovirus-vector PEDF(AV-PEDF group) respectively. The retinal vascular endothelial cells were counted, the PEDF mRNA and protein expression in retina and vitreous were determined by reverse transcriptionpolymerase chain reaction (RT-PCR) and immunohistochemistry. Results After injection with medicine, the number of RNV was decreased obviously in AV-PEDF group（t＝42.009，P lt;0.001）;the protein expression of retinal PEDF was increased obviously in AV-PEDF group（t=36.638，P lt;0.001）; the PEDF mRNA expression in vitreous was also increased obviously in AV-PEDF group （t=9.128，P lt;0.001）. Conclusion Recombinant Adenovirus vector mediated PEDF can raise the PEDF expression in the retinal and vitreous neovascularized tissues in rat, which suggested that the expression of PEDF may be related to inhibition and reduction of RNV. 

Citation： Huiying WANG Wei XU Yunli NIU Zhijian JIANG Ao RONG. Inhabiting effect of pigment epitheliumderived factor on retinal neovascularization mediated by recombinant adenovirus vector. Chinese Journal of Ocular Fundus Diseases, 2008, 24(5): 363-366. doi: Copy