MENG Yan , NIU Yingjun
  • Medical School Hospital of Qingdao University;
Pigment epithelium of eye; Photic stimulation; Wounds and injuries; Erythropoietin; Apoptosis
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective  To assess the protective effect of recombinant human erythropoietin (EPO) on human retinal pigment epithelial (RPE) cells injured by light. Methods  Cultured human RPE cells were exposed to light for 12 hours, and the culture was stopped 24 hours later. The 3(4,5dimethylthiazole2y1)2,5diphenyl tetrazolium bromide (MTT) cell viability assay and annexin V flunorescein isothiocyanate/propidium iodium labeling and flow cytometry were used to assess the effects of EPO with different concentration on the cellular viability and apoptosis of human RPE cells. The protective effect and mechanism of EPO on RPE cells injured by light was detected by adding AG490. Results  EPO, especially with the concentration of 40 IU/ml, obviously increased the cellular viability of RPE cells and apparently decrease the cellular apoptosis induced by light injury. After adding AG490, the effects of EPO on cellular viability and apoptosis were inhibited. Conclusion  It is suggested that EPO can protect the human RPE cells from lightinduced injures, and its protective mechanism works after the combination of EPO and its receptor.

Citation： MENG Yan,NIU Yingjun. Protective effect of erythropoietin on human retinal pigment epithelial cells injured by light. Chinese Journal of Ocular Fundus Diseases, 2007, 23(6): 414-417. doi: Copy