• Department of Ophthalmology, Henan Province People′s Hospital, Zhengzhou 450003, China;
Gene expression regulation; Retinoblastoma/diagnosis; Cell cycle
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Objective To investigate the effect of the 8-bromum-cyclic adenosine monophosphate (8-Br-cAMP) on the telomerase activity and changes of cell cycle in retinoblastoma （RB） cells. Methods The cultured RB cells were divided into the experimental group (8-Br-cAMP) and control group. After cultured for 24, 48 and 72 hours in vitro, the telomerase activity of RB cells was detected by polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) and the changes of cell cycle were detected by flow-cytometry. Results The difference of telomerase activity was significant between the experimental groups and control group (P lt;0.01). There was a negative correlation between the A value of absorbance and the time in the experimental groups (r=-0.778 9, F=33.936, P lt;0.01). The changes of the cell cycle were that the percentages increased in G1 phase and decreased in S phases. Conclusion 8-Br-cAMP may weaken telomerase activity, affect the cell cycle, and inhibit the proliferation of RB cells. (Chin J Ocul Fundus Dis，2004，20：358-360)

Citation： GUO Huili,GUO Xirang,FANG Fengqin. Effect of 8-bromum-cyclic adenosine monophosphate on the telomerase activity and dynamics in retinoblastoma cells. Chinese Journal of Ocular Fundus Diseases, 2004, 20(6): 358-360. doi: Copy