• Department of Ophthalmology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
Vitreoretinopathy, proliferative; Endothelial growth factor; Antisense oligodeoxynucleotides; Disease models, animal
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Objective To evaluate the effect of vascular endothelial cell growth factor (VEGF) antisense oligodeoxynucleotides (ASODNs) on the expression of VEGF in rats with oxygen-induced retinopathy. Methods Thirty newborn Sprague-Dawley (SD) rats were randomly divided into 3 groups:normal control group, disposal group and non-disposed group, The animal models with oxygen-induced proliferative retinopathy were established by raising the rats in hyperoxic environment. Retrobulbar injection was performed with VEGF ASODNs or normal saline on the rats in 3 groups respectively. The intraocular tissues (all the tissues except the cornea, sclera, and lens) and serum were collected, and the expressions of VEGF were determined by using competitive enzyme immunoassay.Results The expressions of VEGF in intraocular tissues of rats in disposal group were significantly lower than those in non-disposed group (P＜0.05), and there was no statistical difference between the disposal and normal control group (P＞0.05). There was no significant difference of the expressions of VEGF in serum of rats between the disposal and non-disposed group (P＞0.05), which were both lower than those in the normal control group (P＜0.05). Conclusion VEGF ASODNs could significantly inhibit the expression of VEGF in intraocular tissues. (Chin J Ocul Fundus Dis，2003，19：172-174)

Citation： DENG Deyong,HAN Lirong,WU Danwei. Effect of vascular endothelial cell growth factor (VEGF) antisense oligodeoxynucleotides on expression of VEGF in rats with oxygen-induced retinopathy. Chinese Journal of Ocular Fundus Diseases, 2003, 19(3): 172-174. doi: Copy