• Department of Ocular Genetics and Molecular Biology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060,China;
Optic nerve diseases; DNA, mitochondrial; DNA mutational analysis
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Objective To investigate the spectrum of mitochondrial DNA (mtDNA) mutations in Chinese patients with Leber′s hereditary optic neuropathy (LHON). Methods The primary mtDNA mutations (G3460A、G11778A and T14484 C) of 140 patients with LHON were detected by mutation-specific priming polymerase chain reaction (MSP-PCR), heteroduplex-single strand conformation polymorphism polymerase chain reaction (HA-SSCP), restriction fragment length polymorphisms (RFLP) and measurement of DNA sequence. The transmissibility of the patients′ stirps was analyzed.Results In the 140 patients with LHON, G11778A mtDNA primary mutation was found in 130 (92.9%), including 113 males and 17 females; G3460A mutation was found in 2 (1.4%) including 1 male and 1 female; G14484A mutation was found in 8 (5.7% ) including 6 males and 2 females.Conclusion In Chinese patients with LHON, the incidence of G11778A mtDNA mutation is higher than that of G3460A and T14484C. (Chin J Ocul Fundus Dis，2003，19：269-332)

Citation： GUO Xiangming,JIA Xiaoyun,XIAO Xueshan,et al.. Spectrum of pathologic mitochondria DNA mutations in Chinese patients with Leber′s hereditary optic neuropathy. Chinese Journal of Ocular Fundus Diseases, 2003, 19(5): 288-291. doi: Copy