XU Qingwen
  • Department of Ophthalmology, Affiliated Hospital of Jining Medical College, Jining 272029, China;
Evans blue/diagnostic use; Blood-retina b arrier; Endothelial growth factors/analysis; Diabetes mellitus
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Objective To verify the effect of Evans blue dye on determining the retina blood vessel leakage． Methods Male Sprague-Dawley rats were used in this study． The VEGF induced retinal blood vessel leakage was checked with Evans blue dye． Then the bloodretina barrier breakdown of 1 week diabetic animals was quantified with Evans blue．The dye was extracted from retina by formamide and the extraction was checked with spect rophotometer． Evans blue leakage was normalized against wet or dry retina weight． Results The retinal Evans blue content of eyes treated with VEGF was remarkably higher than that of the controls (n=17 ,P lt;0．0001)． And the eyes of 1 week diabetic duration animals had more Evans blue dye than that of the normal controls (P lt;0．05)． Conclusion Evans blue dye is a sensitive tracer in quantitatively diagnosing the blood retina barrier breakdown． (Chin J Ocul Fundus Dis, 2001,17:221-223)

Citation： XU Qingwen. The effect of Evans blue on determining the breakdown of blood-retina barrier. Chinese Journal of Ocular Fundus Diseases, 2001, 17(3): 221-223. doi: Copy