XI Xinghua , JIANG Deyong , TANG Luosheng.
  • Department of Ophthalmology,the Second Affiliated Hospital,Hunan Medical University,Changsha 410011,China;
Glycosylation end products，advanced; Retina／drug effects; Thymine nucleotides; Calcium/analysis; Hyperplasia
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Objective To investigate the effects of glycation pro ducts on growth and cytosoic free calcium (［Ca2+］i) of bovine retinal capillary pericytes. Methods The changes of growth and ［Ca2+］i of bovine retinal pericytes,which were cultured in early glycation products of bovine serum albumin (EG-BSA) and advanced glycation end products of bovine serum albumin (AGE-BSA),were studied by counting cell numbers,MTT colorimetric assay,［3H］thymidine incorporating,and fluorescent indicator fura-2 acetoxymeth1 ester (Fura-2AM). Results The number of alive pericytes in groups of EG-BSA and AGE-BSA were 17.87 plusmn;2.36 and 14.77 plusmn;3.72 which comparing with their control groups (20.54 plusmn;0.82 and 20.31 plusmn;0.93)were de creased 13．00% and 27．00% (P lt;0.01) by counting cell numbers on a counting plate after four days.The results were 0.4619 plusmn;0.0946 and 0.3884 plusmn;0.1031 which comparing with their control groups (0.5236 plusmn;0.0539 and 0.5227 plusmn;0.0519)were decreased 12．00% and 25.70% (P lt;0.01) by MTT colorimetric assay.Amount of ［3H］thymidine incorporating in groups of EG-BSA and AGE-BSA were 39450.16 plusmn;887 0.68 and 33667.85 plusmn;10581.70 which comparing with their control groups (56373.63 plusmn;2317.97 and 56542.04 plusmn;1961.23)were decreased 30．00% and 40.40% (P lt;0.01).The ［Ca2+］i concentration of pericytes in groups of EG-BSA and AGE-BSA were (129.55 plusmn;30.41) nmol/L and (179.71 plusmn;56.69) nmo l/L which comparing with their control groups ［(79.70 plusmn;6.94) nmol/L and (83. plusmn;6.39) nmo l/L］ were increased to 163．00% and 214．00%. Conclusion Both EG-BSA and AGE-BSA can inhibit the proliferation and DNA syntheses of retinal capillary pericytes,and increased ［Ca2+］i concentration in pericytes,especially the AGE-BSA. (Chin J Ocul Fundus Dis，2000，16：139-212)

Citation： XI Xinghua,JIANG Deyong,TANG Luosheng.. Effects of glycation products on growth and cytosolic free calcium in bovine retinal capillary pericytes. Chinese Journal of Ocular Fundus Diseases, 2000, 16(3): 169-172. doi: Copy