OBJECTIVE:To observe the effect of dexamethasone to intracellular free Ca2+ of frozen RPE cells. METHODS:The cultured human RPE cells were frozen for 30s at --70 deg;C. The RPE cells were loaded with Fura-2/AM and analyzed using a digital imaging microscopy system,the effect of dexamethasone to intracellular free Ca2+ was measured at a serial concentration of 40, 60,100,150,200 mu;g/ml. RESULTS:The concentration of intracellular free Ca in frozen human RPE cells was increased to 18.6%~29.8% by dexamethasone at concenlration of 40 mu;g/ml~60 mu;g/ml,while was decreased to 28.4%~35.2% at 150 mu;g/ml~200 mu;g/ml. CONCLUSIONS:Effect of dexamethasone showed two aspects of effect to frozen cultured human RPE ceils,that it was inhibitor at high concentration and stimulator at low concentration （Chin J Ocul Fundus Dis,1997,13: 86-88）
Citation： Hong Jing,Wu Jingtian.. EFFECT OF DEXAMETHASONE TO INTRACELLULAR FREECa2+ OF FROZEN HUMAN RETINAL PIGMENT EPITHELIAL CELLS IN VITRO. Chinese Journal of Ocular Fundus Diseases, 1997, 13(2): 86-88. doi: Copy