• Zhongshan Ophthalmic Center,Sun Yat-sen University of Medical ciences ,National Ophthalmological Laboratories, Ministry of Public heath,Guangzhou 510060;
TGF-β ; Endotoxins; Uveitis
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PURPOSE: To investigate the influence of transforming growth factor-beta (TGF- beta;) on endotoxin-induced uveitis (EIU). METHODS: TGF-  beta; was abstracted from humam platelets using Bio-gel P-60 chromatography. It was introduced either topically(drops) or intraperitoneally into the SD rats after footpad injection of lipopolysaccbaride(LPS). The inflammation in the anterior uvea was clinically evaluated with slitlamp every day. RESULTS :TGF- beta; obtained from Biogel P-60 chromatography displayed single band on SDS-PAGE,showing a molecular weight of 12 500 EIU occurs significantly earlier and more severe in the rats which only received LPS injection than in the TGF- beta; treated rats. The duration of the inflammation was also much longer in the untreated rats than in the treated rats (P＜0. 001). CONCLUSION: TGF- beta; purified from human platelets may partly prevent the development of EIU and effectively reduce the severity of the inflammation induced by LPS injection. （Chin J Ocul Fundus Dis,1996,12: 101-104)

Citation： Yang Peizeng,Liang Shunwei,Gong Xiangming,et al. PURIFICATION OF TRANSFORMING GROWTH FACTOR-BETA AND ITS APPLICATION FOR THE PREVENTION AND TREATMENT OF ENDOTOXIN INDUCED UVEITIS. Chinese Journal of Ocular Fundus Diseases, 1996, 12(2): 101-104. doi: Copy