Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury_West China Medical Journal

Authors：

XU Guangmin ,  LAN Zhixun

Department of Anesthesia, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, Sichuan 610072, P. R. China;

Corresponding author：

LAN Zhixun, Email: xgmin2004@163.com

Keywords：

异氟醚; 缺血-再灌注损伤; 肝脏; 细胞间黏附分子-1; 谷胱甘肽

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目的　探讨异氟醚通过抑制细胞间黏附分子（ICAM-1）表达参与减轻肝脏缺血-再灌注（IR）损伤的可能调节机制。方法　32只雌性SD大鼠分为4组。A组大鼠行腹腔注射1%戊巴比妥钠40 mg/kg麻醉，进行手术但不阻断入肝血流；B组1%戊巴比妥钠麻醉后行部分肝脏IR；C组大鼠仅接受1.0 MAC异氟醚吸入麻醉，不阻断血流；D组采用1.0 MAC异氟醚麻醉，建立肝脏IR模型。肝脏缺血60 min，再灌注3 h后取肝组织和血液标本，检测血清丙氨酸转氨酶（ALT）和天冬门氨酸转氨酶（AST）、肝组织ICAM-1和肝组织还原型谷胱甘肽（GSH）、脂质过氧化物丙二醛（MDA）和超氧化物歧化酶（SOD）含量。结果　与戊巴比妥钠麻醉比较，采用异氟醚处理后明显降低血清ALT和AST的水平，再灌注肝组织内GSH、SOD含量明显高于而MDA含量降低，同时抑制肝组织ICAM-1的表达。结论　异氟醚麻醉能够有效减轻肝脏IR损伤，抑制氧自由基的生成和释放，具体机制可能与抑制ICAM-1表达致使细胞内GSH含量增加密切相关。

Citation： XU Guangmin,LAN Zhixun. Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury. West China Medical Journal, 2012, 27(11): 1660-1663. doi: Copy

1.	徐广民, 陶国才, 易斌, 等. 异氟醚对大鼠缺血-再灌注损伤肝脏细胞黏附分子-1表达及中性粒细胞浸润的影响[J]. 临床麻醉学杂志, 2010, 26(11): 1004-1006.
2.	黄施伟, 李士通, 王莹恬, 等. 异氟醚预处理对大鼠肝血窦内皮缺血再灌注损伤的影响[J]. 中华麻醉学杂志, 2007, 27(11): 347-351.
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4.	Pattillo CB, Pardue S, Shen X, et al. ICAM-1 cytoplasmic tail regulates endothelial glutathione synthesis through a NOX4/PI3-kinase-dependent pathway[J]. Free Radic Biol Med, 2010, 49(6): 1119-1128.
5.	Pruitt HM, Langston W, Kevil CG, et al. ICAM-1 cross-linking stimulates endothelial glutathione synthesis[J]. Antioxid Redox Signal, 2007, 9(1): 159-164.
6.	Li L, Yan L, Wang Z, et al, Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis[J]. Chin Med J (Engl), 2000, 113(10): 886-888.
7.	Tao KM, Yang LQ, Liu YT, et al. Volatile anesthetics might be more beneficial than propofol for postoperative liver function in cirrhotic patients receiving hepatectomy[J]. Med Hypotheses, 2010, 75(6): 555-557.
8.	Lv X, Wang ZM, Huang SD, et al. Emulsified isoflurane preconditioning reduces lung injury induced by hepatic ischemia/reperfusion in rats[J]. Int J Med Sci, 2011, 8(5): 353-361.
9.	Ko JS, Kim G, Shin YH, et al. The effects of desflurane and isoflurane on hepatic and renal functions after right hepatectomy in living donors[J]. Transplant Proc, 2012, 44(2): 442-444.
10.	Kim M, Park SW, Kim M, et al. Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury[J]. Anesthesiology, 2011, 114(2): 363-373.
11.	王雨, 汤礼军, 戴睿武, 等. p38MAPK信号转导途径对早期缺血再灌注期间离体肝脏ICAM1 mRNA表达的影响[J]. 华西医学, 2009, 24(8): 2087-2091.
12.	葛廷, 王小琦, 廖世奇, 等. 肝缺血再灌注损伤机制及与相关细胞因子关系的研究进展[J]. 甘肃医药, 2010, 29(5): 499-503.
13.	Xiong Y, Uys JD, Tew KD, et al. S-glutathionylation: from molecular mechanisms to health outcomes[J]. Antioxid Redox Signal, 2011, 15(1): 233-270.

1. 　徐广民, 陶国才, 易斌, 等. 异氟醚对大鼠缺血-再灌注损伤肝脏细胞黏附分子-1表达及中性粒细胞浸润的影响[J]. 临床麻醉学杂志, 2010, 26(11): 1004-1006.
2. 　黄施伟, 李士通, 王莹恬, 等. 异氟醚预处理对大鼠肝血窦内皮缺血再灌注损伤的影响[J]. 中华麻醉学杂志, 2007, 27(11): 347-351.
3. 　王旭东, 卢雅立, 王飞, 等. 七氟烷和异氟醚对肝脏缺血再灌注损伤的影响[J]. 中山大学学报(医学科学版), 2008, 29(1): 28-33.
4. 　Pattillo CB, Pardue S, Shen X, et al. ICAM-1 cytoplasmic tail regulates endothelial glutathione synthesis through a NOX4/PI3-kinase-dependent pathway[J]. Free Radic Biol Med, 2010, 49(6): 1119-1128.
5. 　Pruitt HM, Langston W, Kevil CG, et al. ICAM-1 cross-linking stimulates endothelial glutathione synthesis[J]. Antioxid Redox Signal, 2007, 9(1): 159-164.
6. 　Li L, Yan L, Wang Z, et al, Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis[J]. Chin Med J (Engl), 2000, 113(10): 886-888.
7. 　Tao KM, Yang LQ, Liu YT, et al. Volatile anesthetics might be more beneficial than propofol for postoperative liver function in cirrhotic patients receiving hepatectomy[J]. Med Hypotheses, 2010, 75(6): 555-557.
8. 　Lv X, Wang ZM, Huang SD, et al. Emulsified isoflurane preconditioning reduces lung injury induced by hepatic ischemia/reperfusion in rats[J]. Int J Med Sci, 2011, 8(5): 353-361.
9. 　Ko JS, Kim G, Shin YH, et al. The effects of desflurane and isoflurane on hepatic and renal functions after right hepatectomy in living donors[J]. Transplant Proc, 2012, 44(2): 442-444.
10. 　Kim M, Park SW, Kim M, et al. Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury[J]. Anesthesiology, 2011, 114(2): 363-373.
11. 　王雨, 汤礼军, 戴睿武, 等. p38MAPK信号转导途径对早期缺血再灌注期间离体肝脏ICAM1 mRNA表达的影响[J]. 华西医学, 2009, 24(8): 2087-2091.
12. 　葛廷, 王小琦, 廖世奇, 等. 肝缺血再灌注损伤机制及与相关细胞因子关系的研究进展[J]. 甘肃医药, 2010, 29(5): 499-503.
13. 　Xiong Y, Uys JD, Tew KD, et al. S-glutathionylation: from molecular mechanisms to health outcomes[J]. Antioxid Redox Signal, 2011, 15(1): 233-270.

West China Medical Journal

Effects of Isoflurane on the Changes of Oxygen Free Radicals and Intercellular Adhesion Molecule-1 Expression in Rats with Liver Ischemia and Reperfusion Injury

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