Expression and Significance of Phosphoinositide 3-Kinase and Phosphorylated Protein Kinase B in Bladder Urothelial Carcinoma_West China Medical Journal

Authors：

ANG Weibing , ZHU Bin , JIN Yan , XIE Liren , YANG Kaijun

Department of Urology, People’s Hospital of Pingshan, Shenzhen, Guangdong 518118, P. R. China;

Corresponding author：

Keywords：

膀胱尿路上皮癌; 免疫组织化学; 磷脂酰肌醇3-激酶; 磷酸化蛋白激酶B

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目的　研究磷脂酰肌醇3-激酶（PI3K）和磷酸化蛋白激酶B（p-Akt）在人膀胱尿路上皮癌组织中的表达特征及临床意义。方法　2005年6月－2010年7月，采用免疫组织化学法检测40例膀胱尿路上皮癌组织及10例正常膀胱组织PI3K与p-Akt的表达，并对结果进行统计学分析。结果　PI3K和p-Akt在正常膀胱黏膜组织阳性表达率均低于膀胱尿路上皮癌组织中，差异均有统计学意义（P＜0.05）。同一标本中PI3K和p-Akt的表达不具有相关性（r=0.051，P=0.747）。结论　PI3K、p-Akt在膀胱尿路上皮癌中高表达，两者在膀胱尿路上皮癌中共同促其发展，但其在膀胱尿路上皮癌的预后和进展中的作用尚不明确。

Citation： ANG Weibing,ZHU Bin,JIN Yan,XIE Liren,YANG Kaijun. Expression and Significance of Phosphoinositide 3-Kinase and Phosphorylated Protein Kinase B in Bladder Urothelial Carcinoma. West China Medical Journal, 2012, 27(4): 501-503. doi: Copy

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6.	Jiang BH, Liu LZ. AKT signaling in regulating angiogenesis[J]. Curr Cancer Drug Targets, 2008, 8(1): 19-26.
7.	Engelman JA. Targeting PI3K signalling in cancer: opportunities, challenges and limitations[J]. Nat Rev Cancer, 2009, 9(8): 550-562.
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11.	Eblin KE, Bredfeldt TG, Buffington S, et al. Mitogenic signal transduction caused by monomethylarsonous acid in human bladder cells: role in arsenic-induced carcinogenesis[J]. Toxicol Sci, 2007, 95(2): 321-330.
12.	Qiao M, Sheng S, Pardee AB. Metastasis and AKT activation[J]. Cell Cycle, 2008, 7(19): 2991-2996.
13.	Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation[J]. Biochem J, 1998, 335( Pt 1): 1-13.
14.	Song G, Ouyang GL, Bao SD. The activation of Akt/PKB signaling pathway and cell survival[J]. J Cell Mol Med, 2005, 9(1): 59-71.
15.	费洪荣, 王凤泽. PI3K/Akt信号通路的调控与肿瘤血管生成[J]. 生命的化学, 2010, 30(1): 38-41.

1. 　Hartmann W, Digon-Söntgerath B, Koch A, et al. Phosphatidylinositol 3’-kinase/AKT signaling is activated in medulloblastoma cell proliferation and is associated with reduced expression of PTEN[J]. Clin Cancer Res, 2006, 12(10): 3019-3027.
2. 　Nyåkern M, Tazzari PL, Finelli C, et al. Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral blood mononuclear cells from high-risk myelodysplastic syndrome patients[J]. Leukemia, 2006, 20(2): 230-238.
3. 　Gao N, Flynn DC, Zhang Z, et al. G1 cell cycle progression and the expression of G1 cyclins are regulated by PI3K/AKT/mTOR/p70S6K1 signaling in human ovarian cancer cells[J]. Am J Physiol Cell Physiol, 2004, 287(2): C281-C291.
4. 　Chang F, Lee JT, Navolanic PM, et al. Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapy[J]. Leukemia, 2003, 17(3): 590-603.
5. 　Skinner HD, Zheng JZ, Fang J, et al. Vascular endothelial growth factor transcriptional activation is mediated by hypoxia-inducible factor 1alpha, HDM2, and p70S6K1 in response to phosphatidylinositol 3-kinase/AKT signaling[J]. Biol Chem, 2004, 279(44): 45643-45651.
6. 　Jiang BH, Liu LZ. AKT signaling in regulating angiogenesis[J]. Curr Cancer Drug Targets, 2008, 8(1): 19-26.
7. 　Engelman JA. Targeting PI3K signalling in cancer: opportunities, challenges and limitations[J]. Nat Rev Cancer, 2009, 9(8): 550-562.
8. 　Martelli AM, Tabellini G, Borgatti P, et al. Nuclear lipids: new functions for old molecules?[J]. J Cell Biochem, 2003, 88(3): 455-461.
9. 　Vanhaesebroeck B, Leevers SJ, Ahmadi K, et al. Synthesis and function of 3-phosphorylated inositol lipids[J]. Annu Rev Biochem, 2001, 70(11): 535-602.
10. 　Hanada M, Feng J, Hemmings BA. Structure, regulation and function of PKB/AKT--a major therapeutic target[J]. Biochim Biophys Acta, 2004, 1697(1-2): 3-16.
11. 　Eblin KE, Bredfeldt TG, Buffington S, et al. Mitogenic signal transduction caused by monomethylarsonous acid in human bladder cells: role in arsenic-induced carcinogenesis[J]. Toxicol Sci, 2007, 95(2): 321-330.
12. 　Qiao M, Sheng S, Pardee AB. Metastasis and AKT activation[J]. Cell Cycle, 2008, 7(19): 2991-2996.
13. 　Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation[J]. Biochem J, 1998, 335( Pt 1): 1-13.
14. 　Song G, Ouyang GL, Bao SD. The activation of Akt/PKB signaling pathway and cell survival[J]. J Cell Mol Med, 2005, 9(1): 59-71.
15. 　费洪荣, 王凤泽. PI3K/Akt信号通路的调控与肿瘤血管生成[J]. 生命的化学, 2010, 30(1): 38-41.

West China Medical Journal

Expression and Significance of Phosphoinositide 3-Kinase and Phosphorylated Protein Kinase B in Bladder Urothelial Carcinoma

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