目的 研究糖尿病、阳痿、慢性肾炎患者中筛选出的典型肾阳虚证病例的转录组学特征,揭示出肾阳虚证“同证异治”的生物学基础。 方法 分别对9例肾阳虚证患者进行Agilent人444k表达谱芯片实验,对差异表达基因进行基因本休论(GO)、Pathway分析。 结果 找出332条共同差异表达基因,其中有注释的基因为181条。通过GO分析发现肾阳虚证在免疫系统、氨基酸分解和合成、脂类代谢、生殖、能量代谢及肿瘤相关的基因有密切联系,通过Pathway分析发现与肾阳虚证相关的信号通路有39个。 结论 肾阳虚证可能导致免疫系统低下,氨基酸分解和合成、脂类代谢、生殖、能量代谢功能降低,以及与肿瘤形成相关。
Citation:
YAN Shilin,SHEN Hongchun,WANG Haozhong,TAO Yi. Research on the Transcriptome Features of Kidney-Yang Deficiency Syndrome. West China Medical Journal, 2012, 27(4): 534-537. doi:
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- 2. Stone R. From the ignoble to the sublime[J]. Science, 2004, 305(5684): 631.
- 3. Adler EM. Signaling breakthroughs of the year[J]. Sci STKE, 2012, 5(205): 1.
- 4. Zhang EE, Chapeau E, Hagihara K, et al. Neuronal Shp2 tyrosine phosphatase controls energy balance and metabolism[J]. Proc Natl Acad Sci USA, 2004, 101(45): 16064.
- 5. 严石林,高锋,吴斌,等.肾阳虚证半定量化操作标准的研究[J].现代中西医结合杂志, 2004,13(6): 701-702.
- 6. Casella G, Roger L. Berger, statistical inference[J]. California: Brooks/Cole , 2008: 8.
- 7. Tusher V. Tibshirani R, Chu G. Signifecance analysis of macroarrays applied to the ionizing radiation response[J]. PNAS, 2001, 98(223): 5116-5121.
- 8. Fisher RA. On the interpretation of χ2 from contingency tables, and the calculation of P[J]. J Royal Stat Soci, 85(1): 87-94.
- 9. Storey JD. The positive false discoverty rate: a Bayesian interpretation and the q-value[J]. Annals Stat, 31(6): 2013-2035.
- 10. 郑鹏生,冀静. mTOR信号通路与肿瘤的研究进展西安交通大学学报(医学版)[J]. 2010, 31(1): 88-89.
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