Research on the Transcriptome Features of Kidney-Yang Deficiency Syndrome_West China Medical Journal

Authors：

YAN Shilin ^1,2 ,  SHEN Hongchun ¹ , WANG Haozhong ¹ , TAO Yi ¹

1. Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, P. R. China; 2. Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

SHEN Hongchun, Email: ysl5356@163.com

Keywords：

肾阳虚证; 转录组学; 同证异治

Video：

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目的　研究糖尿病、阳痿、慢性肾炎患者中筛选出的典型肾阳虚证病例的转录组学特征，揭示出肾阳虚证“同证异治”的生物学基础。方法　分别对9例肾阳虚证患者进行Agilent人444k表达谱芯片实验，对差异表达基因进行基因本休论（GO）、Pathway分析。结果　找出332条共同差异表达基因，其中有注释的基因为181条。通过GO分析发现肾阳虚证在免疫系统、氨基酸分解和合成、脂类代谢、生殖、能量代谢及肿瘤相关的基因有密切联系，通过Pathway分析发现与肾阳虚证相关的信号通路有39个。结论　肾阳虚证可能导致免疫系统低下，氨基酸分解和合成、脂类代谢、生殖、能量代谢功能降低，以及与肿瘤形成相关。

Citation： YAN Shilin,SHEN Hongchun,WANG Haozhong,TAO Yi. Research on the Transcriptome Features of Kidney-Yang Deficiency Syndrome. West China Medical Journal, 2012, 27(4): 534-537. doi: Copy

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10. 　郑鹏生,冀静. mTOR信号通路与肿瘤的研究进展西安交通大学学报（医学版）[J]. 2010, 31(1): 88-89.

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1. 　Ray LB, adler EM, Gough NR. Common signaling themes[J]. Science, 2004, 306(5701): 1505.

2. 　Stone R. From the ignoble to the sublime[J]. Science, 2004, 305(5684): 631.

3. 　Adler EM. Signaling breakthroughs of the year[J]. Sci STKE, 2012, 5(205): 1.

4. 　Zhang EE, Chapeau E, Hagihara K, et al. Neuronal Shp2 tyrosine phosphatase controls energy balance and metabolism[J]. Proc Natl Acad Sci USA, 2004, 101(45): 16064.

5. 　严石林，高锋，吴斌，等.肾阳虚证半定量化操作标准的研究[J].现代中西医结合杂志， 2004,13(6): 701-702.

6. 　Casella G, Roger L. Berger, statistical inference[J]. California: Brooks/Cole , 2008: 8.

7. 　Tusher V. Tibshirani R, Chu G. Signifecance analysis of macroarrays applied to the ionizing radiation response[J]. PNAS, 2001, 98(223): 5116-5121.

8. 　Fisher RA. On the interpretation of χ2 from contingency tables, and the calculation of P[J]. J Royal Stat Soci, 85(1): 87-94.

9. 　Storey JD. The positive false discoverty rate: a Bayesian interpretation and the q-value[J]. Annals Stat, 31(6): 2013-2035.

10. 　郑鹏生,冀静. mTOR信号通路与肿瘤的研究进展西安交通大学学报（医学版）[J]. 2010, 31(1): 88-89.

11. 　沈自尹, 黄建华, 陈伟．以药测证对肾虚和肾阳虚大鼠基因表达谱的比较研究[J]．中国中西医结合杂志, 2007, 27(2)：135-137.

12. 　Yang LP, Wang MQ, Wu W. Transcriptome analysis of cold syndrome using microarray[J]. Am J Chi Med, 2007, 35(4): 615-626.

West China Medical Journal

Research on the Transcriptome Features of Kidney-Yang Deficiency Syndrome

Abstract Full text Figures/Tables Video References Cited by

Format

Content

1.	Ray LB, adler EM, Gough NR. Common signaling themes[J]. Science, 2004, 306(5701): 1505.
2.	Stone R. From the ignoble to the sublime[J]. Science, 2004, 305(5684): 631.
3.	Adler EM. Signaling breakthroughs of the year[J]. Sci STKE, 2012, 5(205): 1.
4.	Zhang EE, Chapeau E, Hagihara K, et al. Neuronal Shp2 tyrosine phosphatase controls energy balance and metabolism[J]. Proc Natl Acad Sci USA, 2004, 101(45): 16064.
5.	严石林，高锋，吴斌，等.肾阳虚证半定量化操作标准的研究[J].现代中西医结合杂志， 2004,13(6): 701-702.
6.	Casella G, Roger L. Berger, statistical inference[J]. California: Brooks/Cole , 2008: 8.
7.	Tusher V. Tibshirani R, Chu G. Signifecance analysis of macroarrays applied to the ionizing radiation response[J]. PNAS, 2001, 98(223): 5116-5121.
8.	Fisher RA. On the interpretation of χ2 from contingency tables, and the calculation of P[J]. J Royal Stat Soci, 85(1): 87-94.
9.	Storey JD. The positive false discoverty rate: a Bayesian interpretation and the q-value[J]. Annals Stat, 31(6): 2013-2035.
10.	郑鹏生,冀静. mTOR信号通路与肿瘤的研究进展西安交通大学学报（医学版）[J]. 2010, 31(1): 88-89.
11.	沈自尹, 黄建华, 陈伟．以药测证对肾虚和肾阳虚大鼠基因表达谱的比较研究[J]．中国中西医结合杂志, 2007, 27(2)：135-137.
12.	Yang LP, Wang MQ, Wu W. Transcriptome analysis of cold syndrome using microarray[J]. Am J Chi Med, 2007, 35(4): 615-626.