Determination of Glibenclamide Concentration in Placental Perfusion Medium by High Performance Liquid Chromatography_West China Medical Journal

Authors：

AN Chen , YAO Qin , HUANG Hua ,  ZHANG Jun

Clinical Pharmacy Center, the First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, P. R. China;

Corresponding author：

ZHANG Jun, Email: yanchen126@qq.com

Keywords：

格列苯脲; 高效液相色谱法; 胎盘灌流液; 检测

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目的　建立测定胎盘灌流液中格列苯脲浓度的高效液相色谱（HPLC）检测方法。方法　采用的色谱柱为Symmetry Shield RP C18（150 mm×4.6 mm，5 μm），柱温40℃，流动相为NaH2PO4缓冲盐（25 mmol/L，pH值5.2）︰乙腈=1︰1；内标为格列齐特，流速1.0 mL/min，检测波长228 nm，采用液-液萃取预处理方法测定胎盘灌流液中格列苯脲的浓度。结果　格列苯脲浓度线性范围为2.0～25.0 μg/mL，线性方程为：y=0.226x+0.002，r=0.999 9 （n=6），日内相对标准偏差（RSD）＜3.1%，日间RSD＜9.5%，方法学回收率为95.32%～103.35%。结论　HPLC检测方法灵敏、简便，可用于胎盘灌流液中格列苯脲浓度的检测。

Citation： AN Chen,YAO Qin,HUANG Hua,ZHANG Jun. Determination of Glibenclamide Concentration in Placental Perfusion Medium by High Performance Liquid Chromatography. West China Medical Journal, 2012, 27(6): 806-808. doi: Copy

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1. 　Nanovskaya TN, Patrikeeva S, Hemauer S, et al. Effect of albumin on transplacental transfer and distribution of rosiglitazone and glyburide[J]. J Matern Fetal Neonatal Med, 2008, 21(3): 197-207.
2. 　Kraemer J, Klein J, Lubetsky A, et al. Perfusion studies of glyburide transfer across the human placenta: implications for fetal safety[J]. Am J Obstet Gynecol, 2006, 195(1): 270-274.
3. 　陈丹霞, 陈小华, 林少达, 等. 52例妊娠糖尿病临床指标对围生期母婴影响的研究分析[J]. 中国病案, 2006, 7(3): 43-44.
4. 　王爱枝, 弋海棠, 吴云霞. 妊娠糖尿病对胎儿的危害[J]. 中国保健(医学导刊), 2006(2): 35.
5. 　Dannis N, Athanasios C. A validated high-performance liquid chromatographic method for the determination of glibenclamide in human plasma and its application to pharmacokinetic studies[J]. Pharm Biomed Anal, 2002, 28(3-4): 653-657.
6. 　黄作君, 黎月玲, 郑企琨, 等. 高效液相色谱法测定人血浆中格列苯脲浓度[J]. 中国医院药学杂志, 2004, 24(1): 22-23.
7. 　石英, 刘松青, 代青. LC-MS和HPLC法测定人血浆中格列苯脲和二甲双胍方法的建立[J]. 第三军医大学学报, 2005, 27(8): 731-733.
8. 　Gedeon C, Kapur B, Aleksa K, et al. A simple and rapid HPLC method for the detection of glyburide in plasma original research communication (analytical)[J]. Clin Biochem, 2008, 41(3): 167-173.
9. 　Valdes Santurio JR, González Porto E. Determination of glibenclamide in human plasma by solid-phase extraction and high-performance liquid chromatography[J]. J Chromatogr B Biomed Appl, 1996, 682(2): 364-370.

West China Medical Journal

Determination of Glibenclamide Concentration in Placental Perfusion Medium by High Performance Liquid Chromatography

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