The Effect of Green Tea Extractive Epigallocatechin Gallate on the Angiogenesis of HepG2 Transplanted Tumor in Nude Mice_West China Medical Journal

Authors：

QIN Jiao ¹ ,  XIAO Xiuli ² , PU Xia ²

1. Department of Pathology, North Sichuan Medical College, Nanchong, Sichuan 637000, P. R. China; 2. Department of Pathology, Luzhou Medical College, Luzhou, Sichuan, P. R. China;

Corresponding author：

XIAO Xiuli, Email: 846623077@qq.com

Keywords：

表没食子儿茶素没食子酸酯; 原发性肝癌; 移植瘤; 缺氧诱导因子1α; 血管内皮生长因子; 微血管密度; 新生血管生成

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目的　通过复制人肝癌细胞株HepG2裸鼠皮下移植瘤模型，观察绿茶提取物表没食子儿茶素没食子酸酯（EGCG）干预对HepG2移植瘤新生血管生成的影响。方法　瘤体接种复制HepG2移植瘤模型，荷瘤裸鼠20只随机分组，实验组给予EGCG溶液每日20 mg/（kg·只），腹腔注射3周，对照组给予等量灭菌注射用水3周，末次用药24 h，后处死裸鼠，剥离移植瘤。常规病理切片观察移植瘤组织结构；逆转录-聚合酶链式反应和免疫组织化学法检测移植瘤缺氧诱导因子-1α（HIF-1α）、血管内皮生长因子（VEGF）mRNA及蛋白表达，并通过检测CD34表达计数瘤组织微血管密度（MVD）。结果　组织病理学观察实验组移植瘤见大量坏死区，瘤体内血管数量明显少于对照组；实验组HIF-1α、VEGF mRNA及蛋白表达水平比对照组均明显下调（P＜0.05），实验组MVD比对照组明显下降（P＜0.05）。结论　EGCG可抑制荷瘤裸鼠HepG2移植瘤新生血管生成。

Citation： QIN Jiao,XIAO Xiuli,PU Xia. The Effect of Green Tea Extractive Epigallocatechin Gallate on the Angiogenesis of HepG2 Transplanted Tumor in Nude Mice. West China Medical Journal, 2012, 27(6): 877-881. doi: Copy

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13.	Poon E, Harris AL, Ashcroft M. Targeting the hypoxia-inducible factor (HIF) pathway in cancer[J]. Expert Rev Mol Med, 2009, 27(2): 11- 26.
14.	Jubb A, Hillan K. Expression of HIF-1 alpha in human tumours[J]. J Clin Pathol, 2005, 58(12): 1344.
15.	Przybylski M. A review of the current research on the role of bFGF and VEGF in angiogenesis[J]. J Wound Care, 2009, 18(12): 516-519.
16.	Appelmann I, Liersch R, Kessler T, et a1. Angiogenesis inhibition in cancer therapy: platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) and their receptors: biological functions and role in malignancy[J]. Recent Results Cancer Res, 2010, 180(3): 978-998.

1. 　Larsen CA, Dashwood RH. Suppression of Met activation in human colon cancer cells treated with (-)-epigallocatechin-3-gallate: minor role of hydrogen peroxide[J]. Biochem Biophys Res Commun, 2009, 389(3): 527-530.
2. 　Zhu BH, Zhan WH, Li ZR, et al. (-)-Epigallocatechin-3-gallate inhibits growth of gastric cancer by reducing VEGF production and angiogenesis[J]. World J Gastroenterol, 2007, 13(8): 1162-1169.
3. 　Farabegoli F, Papi A, Bartolini G, et al. (-)-Epigallocatechin-3-gallate downregulates Pg-P and BCRP in a tamoxifen resistant MCF-7 cell line[J]. Phytomedicine, 2010, 17(5): 356-362.
4. 　Shankar S, Ganapathy S, Hingorani SR, et al. EGCG inhibits growth, invasion, angiogenesis and metastasis of pancreatic cancer[J]. Front Biosci, 2008, 13(1): 440-452.
5. 　赵丽萍, 邵宛芳. 茶叶中EGCG功效研究进展[J]. 中国农学通报, 2007, 23(7): 143-147.
6. 　Suzuki Y, Miyoshi N, Isemura M. Health-promoting effect of green tea[J]. Proc Jpn Acad Ser B Phys Biol Sci, 2012,88(3): 88-101.
7. 　 M Chacko S, Thambi PT, Kuttan R, et a1. Beneficial effects of green tea: a literature review[J]. Chin Med, 2010, 5(1): 13.
8. 　Weidner N. Tumor angiogenesis: review of current applications in tumor prognostication[J]. Semin Diagn Pathol, 1993, 10(4): 302-313.
9. 　Zhu BH, Zhan WH, He YL, et al. Epigallocatechin-3-gallate inhibits growth and angiogenesis of gastric cancer and its molecular mechanism[J]. World J gastroenterol, 2009, 12(1): 82-85.
10. 　Wu H, Xin Y, Xiao Y, et al. Low-dose docetaxel combined with (-)-epigallocatechin-3-gallate inhibits angiogenesis and tumor growth in nude mice with gastric cancer xenografts[J]. Cancer Biother Radiopharm, 2012, 27(3): 204-209.
11. 　Fraga A, Ribeiro R, Medeiros R. Tumor hypoxia: the role of HIF[J]. Actas Urol Esp, 2009, 33(9): 941- 951.
12. 　Nakayama K. Cellular signal transduction of the hypoxia response[J]. J Bio Chem, 2009, 146(6): 757-765.
13. 　Poon E, Harris AL, Ashcroft M. Targeting the hypoxia-inducible factor (HIF) pathway in cancer[J]. Expert Rev Mol Med, 2009, 27(2): 11- 26.
14. 　Jubb A, Hillan K. Expression of HIF-1 alpha in human tumours[J]. J Clin Pathol, 2005, 58(12): 1344.
15. 　Przybylski M. A review of the current research on the role of bFGF and VEGF in angiogenesis[J]. J Wound Care, 2009, 18(12): 516-519.
16. 　Appelmann I, Liersch R, Kessler T, et a1. Angiogenesis inhibition in cancer therapy: platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) and their receptors: biological functions and role in malignancy[J]. Recent Results Cancer Res, 2010, 180(3): 978-998.

West China Medical Journal

The Effect of Green Tea Extractive Epigallocatechin Gallate on the Angiogenesis of HepG2 Transplanted Tumor in Nude Mice

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