Expression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance_West China Medical Journal

Authors：

SHI Tao ¹ , GAO Yan ² , GUO Yonglian ¹

1. Department of Urology; 2. Department of VIP; the Affiliated Xiangyang Hospital of Hubei University of Medicine, Xiangyang, Hubei 441000, P. R. China;

Corresponding author：

Keywords：

组织蛋白酶B; 膀胱移行癌细胞; 表达

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目的　探讨组织蛋白酶B（CB）在膀胱移行细胞癌（TCC）中的表达以及其与TCC浸润的关系。方法　取TCC标本40例，TCC分级Ⅰ级23例，Ⅱ～Ⅲ级17例；表浅型TCC（Tis，Ta，T1期） 25例，浸润型TCC（T2～4期）15例。另取10例正常膀胱组织作为对照。用链霉素抗生物素蛋白-过氧化物酶连接法行CB免疫组织化学染色观察并计算CB阳性细胞百分率。结果　正常膀胱组织中基质无明显着色；在TCC癌组织中，CB可为细胞染色，部分基质亦有染色，部分毛细血管内皮细胞及部分成纤维细胞CB表达阳性，在癌周血管内皮细胞的阳性着色CB表达增强。CB在分级和分期高的癌组织中多为弥散阳性染色。TCC分级Ⅰ级组、TCC分级Ⅱ～Ⅲ级组、TCC分期表浅型组、TCC分期浸润型组及正常对照组的CB阳性细胞百分比分别为10.53% ± 3.76%、21.52% ± 3.58%、11.32% ±2.69%、20.57% ± 3.25%、0.11% ± 0.18%，TCC各组均高于正常对照组（P＜0.01）；TCC分级Ⅱ～Ⅲ级组高于TCC分级Ⅰ级组，TCC分期浸润型组高于TCC分期表浅型组，差异均有统计学意义（P＜0.01）。结论　CB可能成为判断TCC进展和预后的重要指标。

Citation： SHI Tao,GAO Yan,GUO Yonglian. Expression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance. West China Medical Journal, 2012, 27(6): 892-894. doi: Copy

1.	Buck MR, Karustis DG, Day NA, et al. Degradation of extracellular-matrix proteins by human cathepsin B from normal and tumour tissues[J]. Biochem J, 1992, 282(Pt 1): 273-278.
2.	Satoh Y, Higashi T, Nouso K, et al. Cathepsin B in the growth of colorectal cancer: suppressive effect of leupeptin on the growth of DMH-induced rat colon neoplasm[J]. Acta Med Okayama, 1996，50(6): 299-303.
3.	Shuja S, Murnane MJ. Marked increases in cathepsin B and L activities distinguish papillary carcinoma of the thyroid from normal thyroid or thyroid with non-neoplastic disease[J]. Int J Cancer, 1996, 66(4): 420-426.
4.	Werle B, Ebert W, Klein W, et al. Cathepsin B in tumors， normal tissue and isolated cells from the human lung[J]. Anticancer Res, 1994, 14(3A): 1169-1176.
5.	Guenette RS, Mooibroek M, Wong K, et al. Cathepsin B, a cysteine protease implicated in metastatic progression is also expressed during regression of the rat prostate and mammary glands[J]. Eur J Biochem, 1994, 226(2): 311-321.
6.	Sloane BF, Rozhin J, Robinson D, et al. Role for cathepsin B and cystatins in tumor growth and progression[J]. Biol Chem Hoppe Seyler, 1990, 371 Suppl: 193-198.
7.	Sivaparvathi M, McCutcheon I, Sawaya R， et al. Expression of cysteine protease inhibitors in human gliomas and meningiomas[J]. Clin Exp Metastasis, 1996, 14(4): 344-350.
8.	Farinati F, Herszenyi L, plebani M, et al. Increased levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis[J]. Carcinogenesis, 1996, 17(12): 2581-2587.
9.	Sinha AA, Gleason DF, Staley NA, et al. Cathepsin B in angiogenesis of hunan prostate: an immunohistochemical and immunoelectron microscopic analysis[J]. Anat Res, 1995, 241(3): 353-362.
10.	Eiján A, Sandes EO, Riveos MD, et al. High expression of cathepsin B in trasitional bladder carcinoma correlates with tumor invasion[J]. Cancer, 2003, 98(2): 262-268.
11.	Visscher DW, Sloane BF，Sameni M，et al.Clincopathologic significance of cathepsin B immunostaining in transitional neoplasia[J]. Mod Pathol，1994，7(1): 76-81.
12.	Staack A， Koenig F, Daniltichenko D, et al. Cathepsin B, H and activities in urine of patients with transitional cell carcinoma of the bladder[J]. Urology, 2002, 59(2): 308-312.
13.	Eiján AM, Sandes E, Puricelli L, et al. Cathepsin B levels in urine from bladder cancer patients[J]. Oncol Res, 2000, 7(6): 1395-1399.
14.	段建敏, 李智, 张亮, 等. Ki-67和组织蛋白酶B在肾癌癌组织中表达的临床意义[J]. 中华国际医学杂志, 2003, 3(2): 99-102.

1. 　Buck MR, Karustis DG, Day NA, et al. Degradation of extracellular-matrix proteins by human cathepsin B from normal and tumour tissues[J]. Biochem J, 1992, 282(Pt 1): 273-278.
2. 　Satoh Y, Higashi T, Nouso K, et al. Cathepsin B in the growth of colorectal cancer: suppressive effect of leupeptin on the growth of DMH-induced rat colon neoplasm[J]. Acta Med Okayama, 1996，50(6): 299-303.
3. 　Shuja S, Murnane MJ. Marked increases in cathepsin B and L activities distinguish papillary carcinoma of the thyroid from normal thyroid or thyroid with non-neoplastic disease[J]. Int J Cancer, 1996, 66(4): 420-426.
4. 　Werle B, Ebert W, Klein W, et al. Cathepsin B in tumors， normal tissue and isolated cells from the human lung[J]. Anticancer Res, 1994, 14(3A): 1169-1176.
5. 　Guenette RS, Mooibroek M, Wong K, et al. Cathepsin B, a cysteine protease implicated in metastatic progression is also expressed during regression of the rat prostate and mammary glands[J]. Eur J Biochem, 1994, 226(2): 311-321.
6. 　Sloane BF, Rozhin J, Robinson D, et al. Role for cathepsin B and cystatins in tumor growth and progression[J]. Biol Chem Hoppe Seyler, 1990, 371 Suppl: 193-198.
7. 　Sivaparvathi M, McCutcheon I, Sawaya R， et al. Expression of cysteine protease inhibitors in human gliomas and meningiomas[J]. Clin Exp Metastasis, 1996, 14(4): 344-350.
8. 　Farinati F, Herszenyi L, plebani M, et al. Increased levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis[J]. Carcinogenesis, 1996, 17(12): 2581-2587.
9. 　Sinha AA, Gleason DF, Staley NA, et al. Cathepsin B in angiogenesis of hunan prostate: an immunohistochemical and immunoelectron microscopic analysis[J]. Anat Res, 1995, 241(3): 353-362.
10. 　Eiján A, Sandes EO, Riveos MD, et al. High expression of cathepsin B in trasitional bladder carcinoma correlates with tumor invasion[J]. Cancer, 2003, 98(2): 262-268.
11. 　Visscher DW, Sloane BF，Sameni M，et al.Clincopathologic significance of cathepsin B immunostaining in transitional neoplasia[J]. Mod Pathol，1994，7(1): 76-81.
12. 　Staack A， Koenig F, Daniltichenko D, et al. Cathepsin B, H and activities in urine of patients with transitional cell carcinoma of the bladder[J]. Urology, 2002, 59(2): 308-312.
13. 　Eiján AM, Sandes E, Puricelli L, et al. Cathepsin B levels in urine from bladder cancer patients[J]. Oncol Res, 2000, 7(6): 1395-1399.
14. 　段建敏, 李智, 张亮, 等. Ki-67和组织蛋白酶B在肾癌癌组织中表达的临床意义[J]. 中华国际医学杂志, 2003, 3(2): 99-102.

West China Medical Journal

Expression of Cathepsin B in Bladder Transitional Cell Carcinoma Tissues and Its Significance

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