Determination of the Concentration of Metformin in Human Plasma with Liquid Chromatography - Electrospray Tandem Mass_West China Medical Journal

Authors：

FENG Shiyin , YONG Xiaolan , DU Xiaolin , LI Nan , WANG Lantian , HUANG Jua

Department of Clinical Pharmacy, General Hospital of Chengdu Military Region of PLA, Chengdu, Sichuan 610083, P. R. China;

Corresponding author：

Keywords：

液相色谱-串联质谱法; 二甲双胍; 血浆药物浓度

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目的　采用液相色谱-串联质谱法测定人血浆中二甲双胍的浓度。方法　血浆样品用乙腈（含0.1%甲酸）沉淀蛋白后用二氯甲烷反洗后进行分析。使用Agilent C8（75 mm×4.6 mm，3.5 μm）色谱柱。流动相：A泵：5 mmol/L醋酸铵（三乙胺调pH值至7.5），B泵：乙腈。线性梯度洗脱，流速0.4 mL/min。采用电喷雾离子源，多反应离子监测。用于定量分析的离子对二甲双胍为130.2/71.1，内标吗啉胍为172.2/60.2。结果　线性范围为50～2 000 ng/mL，最低定量限为50 ng/mL，预处理回收率为81.7%～98.0%，二甲双胍的基质效应＜9.97%，日内和日间相对标准偏差均＜5.2%。结论　液相色谱-串联质谱法快速、简便、灵敏度高，是一种适用于人血浆中药物浓度的测定及药物动力学和生物利用度研究的方法。

Citation： FENG Shiyin,YONG Xiaolan,DU Xiaolin,LI Nan,WANG Lantian,HUANG Jua. Determination of the Concentration of Metformin in Human Plasma with Liquid Chromatography - Electrospray Tandem Mass. West China Medical Journal, 2012, 27(7): 967-970. doi: Copy

1.	邹建军, 蒋春莲, 简龙海, 等. 盐酸二甲双胍缓释片药物动力学及生物等效性研究[J]. 中南药学, 2003, 1(3): 138-141.
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9.	张丹, 王国才, 黄建权, 等. 液相色谱-串联质谱法测定人血浆中二甲双胍的浓度[J]. 药物分析杂志, 2011, 31(2): 317-321.
10.	冯怡, 梁前, 陈燕芬. 高效液相-质谱联用法测定盐酸二甲双胍血药质量浓度[J]. 中国医院药学杂志, 2007, 27(1): 68-70.
11.	Wang YW, Tang YB, Gu JK, et al. Rapid and sensitive liquid chromatography-tandem mass spectrometric method for the quantitation of metformin in human plasma[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2004, 808(2): 215-219.
12.	Discenza L, D’arienzo C, Olah T, et al. LC-MS/MS method using unbonded silica column and aqueous/methanol mobile phase for the simultaneous quantification of a drug candidate and co-administered metformin in rat plasma[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2010, 878(19): 1583-1589.

1. 　邹建军, 蒋春莲, 简龙海, 等. 盐酸二甲双胍缓释片药物动力学及生物等效性研究[J]. 中南药学, 2003, 1(3): 138-141.
2. 　吴琳华, 刘红梅, 赵红光, 等. 盐酸二甲双胍缓释片的生物等效性评价[J]. 中国药学杂志, 2006, 41(3): 214-216.
3. 　彭英, 丁劲松, 阳利龙, 等. 盐酸二甲双胍缓释片人体相对生物利用度研究[J]. 儿科药学杂志, 2006, 12(4): 7-9, 28.
4. 　厉伟兰, 刘晓东, 谢林, 等. 二甲双胍缓释片人体药代动力学及其相对生物利用度[J]. 江苏药学与临床研究, 2006, 14(2): 72-75.
5. 　Swales JG, Gallagher RT, Denn M, et al. Simultaneous quantitation of metformin and sitagliptin from mouse and human dried blood spots using laser diode thermal desorption tandem mass spectrometry[J]. J Pharm Biomed Anal, 2011, 55(3): 544-551.
6. 　Ding CG, Zhou Z, Ge QH, et al. Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry[J]. Biomed Chromatogr, 2007, 21(2): 132-138.
7. 　王大为, 武洁. 液相色谱-电喷雾质谱联用法测定人血浆中二甲双胍[J]. 药物分析杂志, 2007, 27(9): 1367-1369.
8. 　赵晓华, 宋波, 钟大放, 等. 液相色谱-串联质谱法同时测定人血浆中二甲双胍和格列吡嗪[J]. 药学学报, 2007, 42(10): 1087-1091.
9. 　张丹, 王国才, 黄建权, 等. 液相色谱-串联质谱法测定人血浆中二甲双胍的浓度[J]. 药物分析杂志, 2011, 31(2): 317-321.
10. 　冯怡, 梁前, 陈燕芬. 高效液相-质谱联用法测定盐酸二甲双胍血药质量浓度[J]. 中国医院药学杂志, 2007, 27(1): 68-70.
11. 　Wang YW, Tang YB, Gu JK, et al. Rapid and sensitive liquid chromatography-tandem mass spectrometric method for the quantitation of metformin in human plasma[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2004, 808(2): 215-219.
12. 　Discenza L, D’arienzo C, Olah T, et al. LC-MS/MS method using unbonded silica column and aqueous/methanol mobile phase for the simultaneous quantification of a drug candidate and co-administered metformin in rat plasma[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2010, 878(19): 1583-1589.

West China Medical Journal

Determination of the Concentration of Metformin in Human Plasma with Liquid Chromatography - Electrospray Tandem Mass

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