Effects of Capsaicin on Transient Receptor Potential Vanilloid 1 Channels of Visceral Sensory Neurons in Different-Aged Rats_West China Medical Journal

Authors：

LI Hongqi ¹ , LIAO Daqing ² ,  WANG Rurong ¹

1. Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P. R. China; 2. Laboratory of Anesthesiology and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

WANG Rurong, Email: liredflag@163.com

Keywords：

辣椒素; 内脏感觉神经元; 辣椒素受体; 膜片钳; 大鼠

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【摘要】　目的　探讨辣椒素对不同年龄SD大鼠内脏感觉神经元上辣椒素受体（TRPV1）介导的离子通道的影响。　方法　急性分离7~9 d和21~23 d大鼠迷走神经结状神经节神经元，利用全细胞膜片钳技术在分离的神经元上记录辣椒素激活TRPV1受体后通道电流的变化。　结果　 ①7~9 d和21~23 d大鼠内脏感觉神经元的膜电容分别为（18.57±8.60）和（19.85±9.47） pF，（P gt;0.05）；②辣椒素能够激活7~9 d和21~23 d大鼠内脏感觉神经元上TRPV1并产生相似的内向电流，两组产生的峰电流密度分别为（48.59±18.87）、（55.91±20.52） pA/pF（P gt;0.05）；③反复应用辣椒素使TRPV1受体发生失敏现象。　结论　大鼠内脏感觉神经元的TRPV1受体通道在出生后已经发育成熟，且对辣椒素激活的通道电流有相似的变化。
【Abstract】　Objective　 To investigate the effects of capsaicin on transient receptor potential vanilloid 1 (TRPV1) receptor-mediated ion channel currents of visceral sensory neurons in different-aged Sprague-Dawley rats.　Methods　 We isolated the vagal nodose ganglion neurons of rats at an age of 7-9 days or 21-23 days acutely. With the whole cell patch clamp technique, we recorded the current changes of TRPV1 channels activated by capsaicin.　Results　 ① Membrane capacitances of the visceral sensory neurons were (18.57±8.60) and (19.85±9.47) pF in rats of 7-9 and 21-23 days, respectively (P gt;0.05). ② Capsaicin activated the TRPV1 channels and generated inward currents in all the rats; and the peak current densities of the rats of 7-9 days and 21-23 days were respectively (48.59±18.87) and (55.91±20.52) pA/pF (P gt;0.05). ③ Repeated applications of capsaicin produced a phenomenon of desensitization in TRPV1 channels.　Conclusion　 TRPV1 receptor channels of visceral sensory neurons in rats have matured after birth, and the current changes of TRPV1 channels activated by capsaicin are similar.

Citation： LI Hongqi,LIAO Daqing,WANG Rurong. Effects of Capsaicin on Transient Receptor Potential Vanilloid 1 Channels of Visceral Sensory Neurons in Different-Aged Rats. West China Medical Journal, 2011, 26(2): 161-164. doi: Copy

1.	Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia[J]. Neurosci Lett, 1998, 250(3): 177-180.
2.	Wang RR, Xu FD. Postnatal development of right atrial injection of capsaicin-induced apneic response in rats[J]. J Appl Physiol, 2006, 101(1): 60-67.
3.	孙明洁, 基鹏, 王儒蓉. 右心房注射前列腺素E2对新生大鼠呼吸的影响[J]. 华西医学, 2009, 24(2): 368-370.
4.	Lee LY, Lundberg JM. Capsazepine abolishes pulmonary chemore-flexes induced by capsaicin in anesthetized rats[J]. J Appl Physiol, 1994, 76(5): 1848-1855.
5.	Xu F, Gu QH, Lee LY, et al. Acute hypoxia prolongs the apnea induced by right atrial injection of capsaicin[J]. J Appl Physiol, 2003, 94(4): 1446-1454.
6.	Barbara M, Grzegorz K, Manfred G. Anandamide and methanandamide induce both vanilloid VR1 and cannabinoid CB1 receptor-mediated changes in heart rate and blood pressure in anaesthetized rats[J]. Arch Pharmacol, 2001, 364(6): 562-569.
7.	Wang RR, Xu FD, Zhang JG, et al. Carotid sinus nerve is involved in cardiorespiratory responses to intracarotid injection of capsaicin in the rats[J]. J Appl Physiol, 2006, 100(1): 60-66.
8.	Vellani V, Mapplebeck S, Moriondo A, et al. Protein kinase C activaton potentiates gating of the vanilloid receptor VR1 by capsaicin, proton, heat and anandamide[J]. J Physiol, 2001, 534(3): 8132-8251.
9.	Pan XQ, Gonzalez JA, Chang S, et al. Experimental colitis triggers the release of substance P and calcitonin gene-related peptide in the urinary bladder via TRPV1 signaling pathways[J]. Exp Neurol, 2010, 225(2): 262-273.
10.	Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia. Neurosci Lett, 1998, 250(3): 177-180.
11.	Wu ZZ, Chen SR, Pan HL. Transient receptor potential vanilloid type 1 activation down-regulates voltage-gated calcium channels through calcium-dependent calcineurin in sensory neurons[J]. J Biol Chem. 2005, 280(18): 18142-18151.
12.	Rosenbaum T, Gordon-Shaag A, Munari M, et al. Ca2+/calmodulin modulates TRPV1 activation by capsaicin[J]. J Gen Physiol, 2004, 123(1): 53-62.

1. 　Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia[J]. Neurosci Lett, 1998, 250(3): 177-180.
2. 　Wang RR, Xu FD. Postnatal development of right atrial injection of capsaicin-induced apneic response in rats[J]. J Appl Physiol, 2006, 101(1): 60-67.
3. 　孙明洁, 基鹏, 王儒蓉. 右心房注射前列腺素E2对新生大鼠呼吸的影响[J]. 华西医学, 2009, 24(2): 368-370.
4. 　Lee LY, Lundberg JM. Capsazepine abolishes pulmonary chemore-flexes induced by capsaicin in anesthetized rats[J]. J Appl Physiol, 1994, 76(5): 1848-1855.
5. 　Xu F, Gu QH, Lee LY, et al. Acute hypoxia prolongs the apnea induced by right atrial injection of capsaicin[J]. J Appl Physiol, 2003, 94(4): 1446-1454.
6. 　Barbara M, Grzegorz K, Manfred G. Anandamide and methanandamide induce both vanilloid VR1 and cannabinoid CB1 receptor-mediated changes in heart rate and blood pressure in anaesthetized rats[J]. Arch Pharmacol, 2001, 364(6): 562-569.
7. 　Wang RR, Xu FD, Zhang JG, et al. Carotid sinus nerve is involved in cardiorespiratory responses to intracarotid injection of capsaicin in the rats[J]. J Appl Physiol, 2006, 100(1): 60-66.
8. 　Vellani V, Mapplebeck S, Moriondo A, et al. Protein kinase C activaton potentiates gating of the vanilloid receptor VR1 by capsaicin, proton, heat and anandamide[J]. J Physiol, 2001, 534(3): 8132-8251.
9. 　Pan XQ, Gonzalez JA, Chang S, et al. Experimental colitis triggers the release of substance P and calcitonin gene-related peptide in the urinary bladder via TRPV1 signaling pathways[J]. Exp Neurol, 2010, 225(2): 262-273.
10. 　Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia. Neurosci Lett, 1998, 250(3): 177-180.
11. 　Wu ZZ, Chen SR, Pan HL. Transient receptor potential vanilloid type 1 activation down-regulates voltage-gated calcium channels through calcium-dependent calcineurin in sensory neurons[J]. J Biol Chem. 2005, 280(18): 18142-18151.
12. 　Rosenbaum T, Gordon-Shaag A, Munari M, et al. Ca2+/calmodulin modulates TRPV1 activation by capsaicin[J]. J Gen Physiol, 2004, 123(1): 53-62.

West China Medical Journal

Effects of Capsaicin on Transient Receptor Potential Vanilloid 1 Channels of Visceral Sensory Neurons in Different-Aged Rats

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