Effects of Chronic Hypoxia on Expression of Acid-sensing Ion Channels 3 and 2a of Neurons in Petrosal Ganglions of Rats_West China Medical Journal

Authors：

WANG Qiancheng , CHEN Qi , YANG Yin , ZHAO Shu , CHEN Li ,  ZHENG Yu

Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

ZHENG Yu, Email: wangyuchuyi2@163.com

Keywords：

岩神经节; 慢性缺氧; 酸敏感离子通道; 大鼠

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【摘要】　目的　探讨慢性缺氧对大鼠岩神经节神经元酸敏感离子通道（acid-sensing ion channels，ASICs）亚型3（ASIC3）和亚型2a（ASIC2a）表达的影响。　方法　将12只健康成年SD大鼠随机分为正常组和缺氧组。用免疫组织化学法（PV）观察正常和慢性缺氧大鼠岩神经节神经元ASIC3和ASIC2a的表达。　结果　给予慢性缺氧刺激后，岩神经节ASIC3阳性表达神经元数目增多（P lt;0.05），灰度值降低（P lt;0.05）；而ASIC2a阳性表达神经元数目和灰度值无明显变化（P gt;0.05）。　结论　慢性缺氧可上调大鼠岩神经节神经元ASIC3的表达，而对ASIC2a的表达无明显影响，提示ASIC3和ASIC2a可能在岩神经节对缺氧的反应中起着不同的作用。
【Abstract】　Objective　 To investigate the effects of chronic hypoxia on expression of acid-sensing ion channels (ASIC) 3 and ASIC2a in neurons of petrosal ganglions of rats.　Methods　 A total of 12 SD rats were randomly assigned to control group and hypoxia group. The expressions of ASIC3 and ASIC2a of the neurons in the petrosal ganglions in the two groups were investigated with the immunohistochemical technique.　Results　 The level of positive ASIC3 expression in the petrosal ganglions was higher in the hypoxia group than that in the control group (P lt;0.05); the difference of positive ASIC2a expression levels between the control group and the hypoxia group was not statistically significant (P gt;0.05).　Conclusion　 Chronic hypoxia can significantly increase the expression of ASIC3, but not that of ASIC2a, of the neurons in the petrosal ganglions, suggesting their different roles in mediating a cellular response to chronic hypoxia.

Citation： WANG Qiancheng,CHEN Qi,YANG Yin,ZHAO Shu,CHEN Li,ZHENG Yu. Effects of Chronic Hypoxia on Expression of Acid-sensing Ion Channels 3 and 2a of Neurons in Petrosal Ganglions of Rats. West China Medical Journal, 2011, 26(2): 170-173. doi: Copy

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12.	Johnson MB, Jin K, Minami M, et al. Global ischemia induces expression of acid-sensing ion channel 2a in rat brain[J]. J Cereb Blood Flow Metab, 2001, 21(6): 734-740.
13.	Xu TL, Xiong ZG. Dynamic regulation of acid-sensing ion channels by extracellular and intracellular modulators[J]. Curr Med Chem, 2007, 14(16): 1753-1763.
14.	Mamet J, Lazdunski M, Voilley N. How nerve growth factor drives physiological and inflammatory expressions of acid-sensing ion channel 3 in sensory neurons[J]. J Biol Chem, 2003, 278(49): 48907-48913.
15.	Voilley N, de Weille J, Mamet J, et al. Nonsteroid anti-inflammatory drugs inhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors[J]. J Neurosci, 2001, 21(20): 8026-8033.

1. 　Reeh PW, Kress M. Molecular physiology of proton transduction in nociceptors[J]. Curr Opin Pharmacol, 2001, 1(1): 45-51.
2. 　伍龙军, 徐乐天. 酸敏感离子通道的功能及其相关调控[J]. 生物化学与生物物理学进展, 2003, 3(3): 339-343.
3. 　Duan B, Wu LJ, Yu YQ, et al. Upregulation of acid-sensing ion channel ASIC1a in spinal dorsal horn neurons contributes to inflammatory pain hypersensitivity[J]. J Neurosci, 2007, 27(41): 11139-11148.
4. 　Shimada S, Ueda T, Ishida Y, et al. Acid-sensing ion channels in taste buds[J]. Arch Histol Cytol, 2006, 69(4): 227-231.
5. 　Fukuda T, Ichikawa H, Terayama R, et al. ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia[J]. Brain Research, 2006, 1081(1): 150-155.
6. 　陈祁, 潘际刚, 曹性玲, 等. 酸敏感离子通道在大鼠岩神经节神经元的表达[J]. 华西医学, 2009, 24(6): 1470-1472.
7. 　Babinski K, Catarsi S, Biagini G, et al. Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+[J]. J Biol Chem, 2000, 275 (37): 28519-28525.
8. 　Tan ZY, Lu Y, Whiteis CA, et al. Acid-sensing ion channels contribute to transduction of extracellular acidosis in rat carotid body glomus cells[J]. Circ Res, 2007, 101(10): 1009-1019.
9. 　李丹, 陈海锋, 唐玉红, 等. 酸刺激对大鼠颈动脉体Ⅰ型细胞酸敏感离子通道亚型表达的影响[J]. 生理学报, 2009, 61(1): 43-48.
10. 　Sutherland SP, Benson CJ, Adelman JP, et al. Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons[J]. Proc Natl Acad Sci USA, 2001, 98(2): 711-716.
11. 　Immke DC, McCleskey EW. ASIC3: a lactic acid sensor for cardiac pain[J]. Sci World J, 2001, 1(1): 510-512.
12. 　Johnson MB, Jin K, Minami M, et al. Global ischemia induces expression of acid-sensing ion channel 2a in rat brain[J]. J Cereb Blood Flow Metab, 2001, 21(6): 734-740.
13. 　Xu TL, Xiong ZG. Dynamic regulation of acid-sensing ion channels by extracellular and intracellular modulators[J]. Curr Med Chem, 2007, 14(16): 1753-1763.
14. 　Mamet J, Lazdunski M, Voilley N. How nerve growth factor drives physiological and inflammatory expressions of acid-sensing ion channel 3 in sensory neurons[J]. J Biol Chem, 2003, 278(49): 48907-48913.
15. 　Voilley N, de Weille J, Mamet J, et al. Nonsteroid anti-inflammatory drugs inhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors[J]. J Neurosci, 2001, 21(20): 8026-8033.

West China Medical Journal

Effects of Chronic Hypoxia on Expression of Acid-sensing Ion Channels 3 and 2a of Neurons in Petrosal Ganglions of Rats

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