The Effect of Shenfu Injection on the Wound Healing of Deep Partial-thickness Burn in Rats_West China Medical Journal

Authors：

ZHANG Yange ,  CEN Ying

Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

CEN Ying, Email: zhangyange7801@163.com

Keywords：

参附注射液; 烧伤; 创面愈合; 抗氧化; 大鼠

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【摘要】　目的　探讨中成药参附注射液对大鼠深Ⅱ度烧伤创面愈合的影响。　方法　选用16只Sprague-Dawley（SD）大鼠，随机分为实验组、对照组，每组各8只，建立20%体表面积深Ⅱ度烫伤模型。烫伤后即刻及此后每天，实验组大鼠以参附注射液20 mL/kg腹腔注射给药，1次/d，连续给药5 d；对照组给予注射等剂量的生理盐水。分别于致伤后的7、14 d取创面组织块，光学显微镜观察成纤维细胞及胶原纤维生长情况，电子显微镜观察致伤后14 d成纤维细胞细胞器情况，分别计算烧伤后第7、14天两组的创面愈合率，并观察比较两组创面愈合的时间。　结果　所有选入实验的SD大鼠均存活至实验结束。实验组、对照组术后第7天创面愈合率分别为（36.34±2.55）%及（33.13±2.62）%，两组差异有统计学意义（t=2.486，P=0.027）；实验组、对照组术后第14天创面愈合率分别为（75.71±2.29）%及（72.36±2.85）%，两组差异有统计学意义（t=2.590，P=0.022）；实验组、对照组创面愈合时间分别为（20.88±1.36）、（22.94±2.16） d，两组差异有统计学意义（t=-2.286，P=0.0395）。伤后7、14 d创面组织块切片光学显微镜观察发现，实验组肉芽组织及胶原纤维较多，排列更有序，表皮基底细胞增殖活跃，炎症反应较轻；伤后14 d时电子显微镜观察发现，实验组成纤维细胞的细胞器更丰富，分泌胶原更多，实验组创面愈合情况优于对照组。　结论　腹腔注射参附注射液可以促进烧伤创面的愈合，其可能的机制为清除氧自由基，抗脂质过氧化。
【Abstract】　Objective　 To explore the effect of Shenfu injection on promoting healing of deep partial-thickness burn wound in rats. 　Methods　 Sixteen Sprague-Dawley (SD) rats were randomly selected, and deep partial-thickness burn with 20% of the body surface was inflicted. The rats were randomly divided into experimental group and control group with eight in each group. Rats in the experimental group were treated with abdominal injection of Shenfu injection at a dose of 20 mL/(kg•d) for five days continually, and rats in the control group were treated with the same dose of 0.9% saline solution. The growth of granulation tissue and collagen fibers were evaluated under light microscope at the seventh and the fourteenth day. The growth of fibroblast was observed under transmission electron microscope at the fourteenth day. The cure rate of both groups of rats at the seventh and fourteenth day was calculated. Then we compared their healing time and the cure rate of the traumatic wound respectively.　Results　 All rats had survived until wound healing. The cure rate at the seventh day for the experimental group and the control group was (36.34±2.55)% and (33.13±2.62)%, and their difference was statistical (t=2.486, P=0.027); At the fourteenth day, the cure rate was respectively (75.71±2.29)% and (72.36±2.85)% with a significant difference between each other (t=2.590, P=0.022). The healing time of the experimental group (20.88±1.36) was significantly shorter than that of the control group (22.9±2.16) (t=-2.286, P=0.040). At the seventh and fourteenth day, light microscope observation showed that the growth of granulation tissue and collagen fibers for rats in the experimental group were much more than that in the control group, the basale cell proliferation was more active, and inflammation was slighter. Through transmission electron microscope, we observed more fibroblast and collagen in the experimental group, which showed a better cure than the control group.　Conclusion　 Shenfu injection can significantly promote wound healing of deep partial-thickness burn. It may possibly get this effect through anti-oxidation.

Citation： ZHANG Yange,CEN Ying. The Effect of Shenfu Injection on the Wound Healing of Deep Partial-thickness Burn in Rats. West China Medical Journal, 2011, 26(4): 547-550. doi: Copy

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7.	孟庆涛, 夏中元, 贾一帆, 等. 参附注射液对大鼠肠缺血再灌注时肠上皮细胞casapses-3、bcl-2基因表达的影响[J]. 武汉大学学报(医学版), 2004, 25(3): 275-278.
8.	张璟瑜, 唐作磊, 纳雪晴, 等. 参附注射液对行颅内动脉瘤夹闭术患者血清SOD, MDA, S-100β蛋白的影响[J]. 华西医学, 2009, 24(4): 975-977.
9.	Zheng CD, Min S. Cardioprotection of Shenfu Injection against myocardial ischemia/reperfusion injury in open heart surgery[J]. Chin J Integr Med, 2008, 14(1): 10-16.
10.	李扬, 杨博, 陈绍洋, 等. 参附注射液预处理对大鼠脑缺血-再灌注损伤后HSP70和HSP90 表达的影响[J]. 临床麻醉学杂志, 2010, 26(1): 51-53.
11.	苏泓洁, 向小勇, 赵兴吉, 等. 参附注射液对大鼠心肌缺血再灌注损伤保护作用的实验研究[J]. 重庆医科大学学报, 2010, 35(1): 60-64.
12.	冯世杰, 花兰女, 金曙雯, 等. 大鼠烫伤模型的制作[J]. 上海第二医科大学学报, 1995, 15(2): 195-197.
13.	孙同柱, 付小兵, 顾小曼, 等. 重组人表皮生长因子促进兔烫伤创面愈合的实验研究[J]. 实验动物科学与管理, 2000, 17(4): 12-14.
14.	Arturson GP. Pathophysiology of the burn wound[J]. Ann Chir Gynaecol, 1980, 69(5): 178-190.
15.	吴静, 胡森, 汪剑威, 等. 维生素C对烫伤休克大鼠肠内补液时肠组织氧自由基损伤的影响[J]. 解放军医学杂志, 2008, 33(6): 646-648.
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19.	万敬枝, 程梦琳, 吴基良. 参附注射液对局灶性脑缺血再灌注大鼠脑组织NO、MDA含量和SOD活性的影响[J]. 中国中医急症, 2004, 13(6): 381-382.
20.	吴洋, 夏中元. 参附注射液对肠缺血再灌注大鼠肾组织HO-1与iNOS表达的影响[J]. 中华中医药杂志, 2010, 25(7): 1024-1027.
21.	Zhang XJ, Song L, Zhou ZG, et al. Effect of shenfu injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury[J]. World J Gastroenterol, 2006, 12(27): 4389-4391.

1. 　Clark RA. Cutaneous tissue repair: basic biologic considerations[J]. J Am Acad Dermatol, 1985, 13(5 Pt l): 701-725.
2. 　Haycock JW, Ralston DR, Morris B, et al. Oxidative damage to protein and alterations to antioxidant levels in human cutaneous thermal injury[J]. Burns, 1997, 23(7-8): 533-540.
3. 　Sayan H, Ozacmak VH, Ozen OA, et al. Beneficial effects of melatonin on reperfusion injury in rat sciatic nerve[J]. J Pineal Res, 2004, 37(3): 143-148.
4. 　Thang PT, Patrick S, Teik LS, et al. Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage[J]. Burns, 2001, 27(4): 319-327.
5. 　郑世营, 张晓膺, 王志刚, 等. 参附注射液对离体大鼠缺氧/复氧心肌的保护作用[J]. 中国急救医学, 2004, 24(6): 425-427.
6. 　Cao J, Zheng CD, Zhang GX, et al. Protective effect of Shenfu injection on mitochondria injured by ischemia-reperufsion in Rabbits[J]. Chin Medl J (Engl), 2005, 118(6): 505-507.
7. 　孟庆涛, 夏中元, 贾一帆, 等. 参附注射液对大鼠肠缺血再灌注时肠上皮细胞casapses-3、bcl-2基因表达的影响[J]. 武汉大学学报(医学版), 2004, 25(3): 275-278.
8. 　张璟瑜, 唐作磊, 纳雪晴, 等. 参附注射液对行颅内动脉瘤夹闭术患者血清SOD, MDA, S-100β蛋白的影响[J]. 华西医学, 2009, 24(4): 975-977.
9. 　Zheng CD, Min S. Cardioprotection of Shenfu Injection against myocardial ischemia/reperfusion injury in open heart surgery[J]. Chin J Integr Med, 2008, 14(1): 10-16.
10. 　李扬, 杨博, 陈绍洋, 等. 参附注射液预处理对大鼠脑缺血-再灌注损伤后HSP70和HSP90 表达的影响[J]. 临床麻醉学杂志, 2010, 26(1): 51-53.
11. 　苏泓洁, 向小勇, 赵兴吉, 等. 参附注射液对大鼠心肌缺血再灌注损伤保护作用的实验研究[J]. 重庆医科大学学报, 2010, 35(1): 60-64.
12. 　冯世杰, 花兰女, 金曙雯, 等. 大鼠烫伤模型的制作[J]. 上海第二医科大学学报, 1995, 15(2): 195-197.
13. 　孙同柱, 付小兵, 顾小曼, 等. 重组人表皮生长因子促进兔烫伤创面愈合的实验研究[J]. 实验动物科学与管理, 2000, 17(4): 12-14.
14. 　Arturson GP. Pathophysiology of the burn wound[J]. Ann Chir Gynaecol, 1980, 69(5): 178-190.
15. 　吴静, 胡森, 汪剑威, 等. 维生素C对烫伤休克大鼠肠内补液时肠组织氧自由基损伤的影响[J]. 解放军医学杂志, 2008, 33(6): 646-648.
16. 　Tanaka H, Matsuda T, Miyagantani Y, et al. Reduction of resuscitation fluid volumes in severely burned patients using ascorbic acid administration: a randomized, prospective study[J]. Arch Surg, 2000, 135(3): 326-331.
17. 　Horton JW. Free radicals and lipid peroxidation mediated injury in burn trauma: the role of antioxidant therapy[J]. Toxicology, 2003, 189(1-2): 75-88.
18. 　Vorauer-Uhl K, Fürnschlief E, Wagner A, et al. Topically applied liposome encapsulated superoxide dismutase reduces postburn wound size and edema formation[J]. Eur J Pharm Sci, 2001, 14(1): 63-67.
19. 　万敬枝, 程梦琳, 吴基良. 参附注射液对局灶性脑缺血再灌注大鼠脑组织NO、MDA含量和SOD活性的影响[J]. 中国中医急症, 2004, 13(6): 381-382.
20. 　吴洋, 夏中元. 参附注射液对肠缺血再灌注大鼠肾组织HO-1与iNOS表达的影响[J]. 中华中医药杂志, 2010, 25(7): 1024-1027.
21. 　Zhang XJ, Song L, Zhou ZG, et al. Effect of shenfu injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury[J]. World J Gastroenterol, 2006, 12(27): 4389-4391.

West China Medical Journal

The Effect of Shenfu Injection on the Wound Healing of Deep Partial-thickness Burn in Rats

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