Study on the Development of Acute Myocardial Infarction Model in Old Rats_West China Medical Journal

Authors：

LIANG Yanhong ¹ , MIAO Guobin ¹ ,  ZHANG Jian ²

1. General Department, Beijing Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020; 2.Beijing Youyi Hospital, Capital University of Medical Sciences, Beijing 100050, P. R. China;

Corresponding author：

ZHANG Jian, Email: yi125@sina.com

Keywords：

老年大鼠; 急性心肌梗死; 血流动力学; 动物模型

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【摘要】　目的　用老年大鼠建立一种与人类发病年龄相符的稳定可重复的急性心肌梗死动物模型。　方法　 Wistar大鼠经戊巴比妥钠麻醉后，气管切开插管，连通呼吸机，开胸后结扎左冠状动脉前降支。分不同时间点测血流动力学后取出心脏行病理组织学观察。　结果　成功建立老年大鼠心肌梗死模型并进行病理组织学验证。　结论　建立老年大鼠心梗模型是可靠的，有助于指导临床实践。
【Abstract】　Objective　 To develop a steady and repeatable acute myocardial infarction model in old rats in accordance to human age.　Methods　 Wistar rats were anaesthetized with sodium phenobarbital. After tracheotomy intubation, respiration machine was linked. Left anterior thoracotomy was performed to ligate the left anterior descending coronary artery. After hemodynamic parameters were obtained at various time points, hearts of the rats were taken for pathologic and histological examinations.　Results　 The model of acute myocardial infarction in old rats was established successfully and proved by pathologic and histological staining.　Conclusion　 It is reliable to establish the myocardial infarction model in old rats, which is helpful in guiding clinical practices.

Citation： LIANG Yanhong,MIAO Guobin,ZHANG Jian. Study on the Development of Acute Myocardial Infarction Model in Old Rats. West China Medical Journal, 2011, 26(7): 1025-1027. doi: Copy

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10.	王飞燕, 李学文, 仝凌. 影响大鼠心肌梗死模氆存活率的因素分析[J]. 中西医结合心脑血管病杂志, 2008, 6(7): 862-863.
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1. 　刘元生, 陈运贞. 慢性心肌梗塞大鼠实验模型[J]. 重庆医科大学学报, 2002, 27(2): 153-155.
2. 　刘尊齐, 崔连群, 盖玉生. 大鼠急性心肌梗死模型的制备[J]. 基础医学与临床, 2007, 7(9): 34-35.
3. 　赵静, 邵素霞, 张雷, 等. 改良法制备大鼠心肌梗死模型[J]. 河北医科大学学报, 2010, 31(1): 1-4.
4. 　Liu L, Azhar G, Gao W, et al. Bcl-2 and Bax expression in adult rat hearts after coronary occlusion: age-associated differences[J]. Am J Physiol, 1998, 275(1): 315-322.
5. 　Azhar G, Gao W, Liu LX, et al. Ischemia-reperfusion in the adult mouse heart:influence of age[J]. Exp Gerontol, 1999, 286(34): 699-714.
6. 　Kajstura J, Cheng W, Sarangarajan R, et al. Necrotic and apoptotic myocyte cell death in the aging heart of Fischer 344 rats[J]. Am J Physiol Heart Circ Physiol, 1996, 271(5): 1215-1228.
7. 　Bhind R, Witting PK, Mcahon AC, et al. Rat models of myocardial infarction. Pathogenetic insights and clinical relevance[J]. Thromb Haemost, 2006, 96(5): 602-610.
8. 　Tarnavski O, McMullen JR, Schinke M, et al. Mouse cardiac surgery: comprehensive techniques for the generation of mouse models of human diseases and their application for genomic studies[J]. Physiol Genomics, 2004, (16): 349-360.
9. 　李保, 马业新, 解军, 等. 大鼠心肌梗死模型制备的改进[J]. 山西医科大学学报, 2005, 36(1): 75-78.
10. 　王飞燕, 李学文, 仝凌. 影响大鼠心肌梗死模氆存活率的因素分析[J]. 中西医结合心脑血管病杂志, 2008, 6(7): 862-863.
11. 　Ramunddal T, Lorentzon M, Omerovie E. Decreased mortality in a rat model of acute postinfarction heart failure[J]. Biochem Biophys Res Commun, 2006, 341(2): 459-463.
12. 　Kajstura J, Cheng W, Reiss K, et al. Apoptotic and necrotic myocyte cell death are independent contributing variables of infarct size in rats[J]. Laboratory Investigation, 1996, 74(1): 86-107.

West China Medical Journal

Study on the Development of Acute Myocardial Infarction Model in Old Rats

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