Effect of Serum of Pregnant patients with Intrahepatic Cholestasis on the Excretion of Corticotropin-Releasing Hormone in the Placental Tissue in Vitro_West China Medical Journal

Authors：

1. Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China; 2. Department of Obstetrics and Gynecology, Beijing Aerospace General Hospital, Beijing 100076, P.R.China;

Corresponding author：

Keywords：

胎盘; 促肾上腺皮质激素释放激素; 妊娠肝内胆汁淤积症

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

【摘要】目的 观察妊娠肝内胆汁淤积症（intrahepatic cholestasis of pregnancy，ICP）患者血清对体外培养的胎盘组织促肾上腺皮质激素释放激素（corticotropin-releasing hormone，CRH）的分泌水平的影响。方法收集2005年3月-7月在四川大学华西第二医院产科住院分娩的正常妊娠妇女胎盘组织及其血清（对照组）与ICP患者血清（ICP组）各10例。通过胎盘组织培养及放射免疫法测定其培养液中CRH水平。结果 ICP组胎盘组织CRH分泌水平低于对照组，ICP组24、48、72、96 h分别为（84.95±34.98）、（74.57±29.93）、（71.16±27.26）、（81.07±37.18） pg/mL；对照组分别为（103.74±30.85）、（108.27±23.77）、（109.20±23.81）、（118.15±26.84） pg/mL。两组比较，48h后差异有统计学意义（P lt;0.05）。结论 ICP患者血清对体外培养的胎盘组织CRH分泌有抑制作用。
【Abstract】 Objective To observe the effect of serum of pregnant patients with intrahepatic cholestasis (ICP) on the excretion level of corticotrophin-releasing hormone in the placental tissue in vitro. Methods Serum from 10 patients with ICP (ICP group) and from the healthy placental tissue of 10 normal people (control group) were collected from March to July, 2005. Cell culture and radioimmunoassay methods were used to investigate the corticotropin-releasing hormone (CRH) levels in placental tissue. Results The level of CRH in human placental tissue treated with sera of ICP was lower than that in the control group. 24, 48, 72, and 96 hours after treated with the serum, the levels of CRH in the ICP group were (84.95±34.98), (74.57±29.93), (71.16±27.26), and (81.07±37.18) pg/mL, respetively;while in the control group were (103.74±30.85), (108.27±23.77), (109.20±23.81), and (118.15±26.84) pg/mL, respectively. There was significant difference in the levels of CRH between ICP group and control group 48 hours after the culture (P lt;0.05). Conclusion The serum from the patients with ICP may inhibit the excretion of CRH in the placental tissue.

Citation： CHEN Jiangli,WANG Xiaodong,XING Aiyun,LIU Shuyun. Effect of Serum of Pregnant patients with Intrahepatic Cholestasis on the Excretion of Corticotropin-Releasing Hormone in the Placental Tissue in Vitro. West China Medical Journal, 2010, 25(9): 1657-1658. doi: Copy

1.	Challis JR, Matthews SG, Van Meir C, et al. Current topic: the placental corticotropin-releasing hormone-adrenocorticotrop in axis[J]. Placenta, 1995, 16 (6): 481-502.
2.	王晓东, 余海燕, 姚强, 等. 胎盘小叶胎儿血管系统CRH及其受体CRH-R1的表达[J]. 四川大学学报(医学版), 2005, 36(5): 634-636.
3.	Petraglia F, Coukos G, Volpe A, et al. Involvement of placental neurohorm ones in human parturition[J]. Ann N Y Acad Sci, 1991, 622: 331-340.
4.	Wetzka B, Sehringer B, Schfer WR, et al. Expression patterns of CRH, CRH receptors, and CRH binding protein in human gestational tissue at term[J]. Exp Clin Endocrinol Diabetes, 2003, 111(3): 154-161.
5.	Robidoux J, Simoneau L, Masse A, et al. Activation of L-Type calcium channels induces corticotropin-releasing factor secretion from human placental trophoblasts[J]. J Clin Endocrinol Metab, 2000, 85(9): 3356-3364.
6.	Jain V, Shi SQ, Vedernikov YP, et al. In vivo effects of corticotropin releasing factor in pregnant rats[J]. Am J Obstet Gynecol, 1998, 178 (1 pt 1): 186-191.
7.	Clifton VL, Read MA, L eitch IM, et al. Corticotropin-releasing hormone induced vasodilatation in the human fetal placental circulation[J]. J Clin Endocrinol Metab, 1994, 79 (2): 666-669.

1. Challis JR, Matthews SG, Van Meir C, et al. Current topic: the placental corticotropin-releasing hormone-adrenocorticotrop in axis[J]. Placenta, 1995, 16 (6): 481-502.
2. 王晓东, 余海燕, 姚强, 等. 胎盘小叶胎儿血管系统CRH及其受体CRH-R1的表达[J]. 四川大学学报(医学版), 2005, 36(5): 634-636.
3. Petraglia F, Coukos G, Volpe A, et al. Involvement of placental neurohorm ones in human parturition[J]. Ann N Y Acad Sci, 1991, 622: 331-340.
4. Wetzka B, Sehringer B, Schfer WR, et al. Expression patterns of CRH, CRH receptors, and CRH binding protein in human gestational tissue at term[J]. Exp Clin Endocrinol Diabetes, 2003, 111(3): 154-161.
5. Robidoux J, Simoneau L, Masse A, et al. Activation of L-Type calcium channels induces corticotropin-releasing factor secretion from human placental trophoblasts[J]. J Clin Endocrinol Metab, 2000, 85(9): 3356-3364.
6. Jain V, Shi SQ, Vedernikov YP, et al. In vivo effects of corticotropin releasing factor in pregnant rats[J]. Am J Obstet Gynecol, 1998, 178 (1 pt 1): 186-191.
7. Clifton VL, Read MA, L eitch IM, et al. Corticotropin-releasing hormone induced vasodilatation in the human fetal placental circulation[J]. J Clin Endocrinol Metab, 1994, 79 (2): 666-669.

West China Medical Journal

Effect of Serum of Pregnant patients with Intrahepatic Cholestasis on the Excretion of Corticotropin-Releasing Hormone in the Placental Tissue in Vitro

Abstract Full text Figures/Tables Video References Cited by

Format

Content