Expression of CyclinD1 in Esophagus Squamous Carcinoma and Its Correlation with CyclinE_West China Medical Journal

Authors：

HUANG Hanxing ¹ , ZHANG Jintian ²

1. Department of Pathology, The First Hospital of Putian, Putian, Fujian 351100, P.R.China; 2. Department of Pathology, Medical School of Putian College, Putian, Fujian 351100, P.R.China;

Corresponding author：

Keywords：

食管癌; CyclinD1; CyclinE; 免疫组织化学; 定量分析

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【摘要】目的 观察CyclinD1在食管鳞状细胞癌和癌周正常食管组织的表达，从量化角度阐明其在食管鳞状细胞癌组织中表达的病理学意义及其与CyclinE表达的关系。方法用免疫组化MaxVision法检测CyclinD1、CyclinE在食管鳞状细胞癌和癌周正常食管组织的表达，Image-pro plus图像分析软件对其表达强度进行定量分析，并用表达的阳性单位（positive unit，PU）反映其表达强度。结果 CyclinD1蛋白在食管鳞状细胞癌癌细胞核中表达的PU值高于正常食管组织（P lt;0.001）；CyclinD1蛋白表达的PU值与食管鳞状细胞癌分化程度有关（P lt;0.001）；浸润浆膜层组的CyclinD1蛋白表达的PU值高于无浆膜层浸润组（P=0.037）；伴淋巴结转移组的CyclinD1蛋白表达的PU值高于无淋巴结转移组（P=0.012）；CyclinD1与CyclinE蛋白在食管鳞状细胞癌中表达呈正相关（r=0.650，P lt;0.01）。结论 CyclinD1在食管鳞状细胞癌中表达上调，且与生物学特性相关，可作为判断肿瘤患者预后不良的标志之一；CyclinD1和CyclinE可能在食管癌癌变过程中起协同作用。
/【Abstract】　Objective　 To observe the expression of CyclinD1 and its correlation with the expression of CyclinE in esophagus squamous carcinoma.　Methods　 The expressions of CyclinD1 and CyclinE protein were detected by immunohistochemistry (MaxVision method) using the monoclonal antibody and their intensities were assessed quantitatively by Image-pro plus image analysis system.　Results　 The monoclonal antibody was detected in specific cell nucleus. The positive unit (PU) value of CyclinD1 was larger in the esophagus squamous carcinoma than that in the normal esophagus tissue with a statistically significant difference (P lt;0.001). The expression of CyclinD1 was correlated with differentiation of esophagus squamous carcinoma (P lt;0.001) and infiltration degree of esophagus squamous carcinoma (P=0.037). The PU value of CyclinD1 of the nuclei was larger in esophagus squamous carcinoma with lymph node metastasis than those without lymph node metastasis (P=0.012). There was a positive correlation between CyclinD1 protein and CyclinE protein (r=0.650, P lt;0.01).　Conclusion　 The positive expression of CyclinD1 is up-regulated in the esophagus squamous carcinoma. CyclinD1 protein relates to the clinicopathological characteristics and prognostic value in esophagus squamous carcinoma. CyclinD1 expression is bly associated with CyclinE expression in esophagus squamous carcinoma.

Citation： HUANG Hanxing,ZHANG Jintian. Expression of CyclinD1 in Esophagus Squamous Carcinoma and Its Correlation with CyclinE. West China Medical Journal, 2010, 25(11): 1944-1946. doi: Copy

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1. 　申洪, 陆药丹. 免疫组织化学染色的定量方法研究[J]. 生物医学工程学杂志, 1993, 10(3): 281-284.
2. 　申洪. 免疫组织显色反应强度定量方法研究(Ⅱ)[J]. 细胞与分子免疫学杂志, 1994, 10(4): 33-35.
3. 　申洪. 免疫组织化学染色的定量方法研究(Ⅲ)[J]. 中国组织化学与细胞化学杂志, 1995, 4(1): 89-92.
4. 　Donnellan R, Chetty R. CyclinD1 and human neoplasia[J]. Mol Pathol, 1998, 51(1): l-7.
5. 　Liu B, Ren Z, Shi Y, et al. Activation of signal transducers and activators of transcription 3 and overexpression of its target gene CyclinD1 in laryngeal carcinomas[J]. Laryngoscope, 2008, 118(11): 1976-1980.
6. 　Tsai-Turton M, Luong BT, Tan Y, et al. Cyclophosphamide-induced apoptosis in COV434 human granulose cells involves oxidative stress and glutathione depletion[J]. Toxicol Sci, 2007, 98(1): 216-230.
7. 　Bilalovic N, Vranic S, Basic H, et al. Immunohistochemical evaluation of CyclinD1 in breast cancer[J]. Croat Med J, 2005, 46(3): 382-384.
8. 　Zhu SC, Li R, Wang YX, et al. Impact of simultaneous assay, the PCNA, cyclinD1, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer possible incorporation into clinical TNM staging system[J]. World J Gastroenterol, 2005, 11(25): 3823-3829.
9. 　胡玉林, 刘志辉, 赵于天, 等. P53和CyclinD1蛋白的表达对食管癌预后意义的研究[J]. 哈尔滨医科大学学报, 2007, 41(1): 52-54.
10. 　金春亭, 张林西, 李海军, 等. 人食管鳞癌中细胞周期调节基因蛋白的表达[J]. 世界华人消化杂志, 2005, 13(15): 1909-1912.
11. 　Fu ZJ, Ma ZY, Wang QR, et al. Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients[J]. Clin Exp Metastasis, 2008, 25(8): 887-892.
12. 　Bahnassy AA, Zekri AR, Houssinis EL, et al. CyclinA and cyclinD, as significant prognostic markers in colorectal patients[J]. BMC Gastroenteral, 2004, 4(1): 22.
13. 　田冬艳, 王德华, 刘永红, 等. Survivin和CyclinD1在卵巢癌中的表达和相关性[J]. 天津医科大学学报, 2009, 15(1): 81-87.
14. 　Shao J, Teraishi F, Katsuda K, et al. p53 inhibits adriamycin-induced down-regulation of cyclin D1 expression in human cancer cells[J]. Biochem Biophys Res Commun, 2002, 290(3): 1101-1107.

West China Medical Journal

Expression of CyclinD1 in Esophagus Squamous Carcinoma and Its Correlation with CyclinE

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