目的：分析体外受精(IVF)患者卵泡液中sFas和sFasL的浓度与妊娠的关系，以及与卵泡液中的促黄体素(LH)水平之间的相关性。方法：收集行IVF/ICSI患者的卵泡液，测定sFas、sFasL和LH浓度。结果：妊娠组排卵前卵泡液中的sFasL和LH水平显著高于未妊娠组(P分别为0.002和＜0.001)，两组中sFas水平没有明显差异。通过logistic回归分析，卵泡液中与妊娠有统计学相关的因素仅为LH，OR=4.117(P为0.001)。卵泡液中的LH和sFasL的水平呈明显正相关关系(P lt;0.001)，LH与sFas之间无显著相关关系。结论：在IVF/ICSI治疗周期中，卵泡液中的sFasL蛋白和LH水平与妊娠结局有关，卵泡液中的LH水平可能通过调节卵泡局部的某些系统来调节卵泡中FasFasL系统介导的凋亡作用。
Citation：
MA Qianhong,LI ShangWei,PENG Zhilan,et al.. Effect of Different Concenitaliors of Soluble Fas，Soluble Fas Ligand and LH in Preovulatory Follicular Fluid on Pregnancy Results in outcome. West China Medical Journal, 2009, 24(2): 319-322. doi:
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Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
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- 2. Srivastava M，Lippes J，Fichorova R，et al.Soluble Fas(sFas)and Soluble Fas ligand(sFasL)in human reproductive tract fluid[M].IN：Processings of the 45th Annual Meeting of the Society for Gynecologic Investigation；Chicago，IL.Abstract T203..
- 3. de los Santos MJ，Anderson DJ，Racowsky C et al.Presence of FasFas ligand system and Bcl2 gene products in cells and fluids from gonadotropinstimulated human ovaries[J].Biol Reprod，2000，63：1811-1816..
- 4. 马黔红，李尚为，黄仲英，等.长效促性腺激素释放激素激动剂用于体外受精胚胎移植中降调节后延迟卵巢启动时间的探讨[J].实用妇产科杂志，2006，22：603-606..
- 5. Kayagaki N，Kawasaki A，Ebata T，et al.Metalloproteinasemediated release of human Fas ligand[J].J Exp Med，1995，192：1777-1783..
- 6. Cataldo NA，Fujimoto VY，Jaffe RB.Interferongamma and activin A promote insulinlike growth factorbinding protein2 and 4 accumulation by human luteinizing granulosa cells，and interferongamma promotes their apoptosis[J].J Clin Endocrinol Metab，1998，83：179-186..
- 7. Quirk SM，Cowan RG，Joshi SG，Henrikson KP.Fas antigenmediated apoptosis in human granulosa/luteal cells[J].Biol Reprod，1995，52：279-287..
- 8. Porter DA，Harman RM，Cowan RG，et al.Susceptibility of ovarian granulosa cells to apoptosis differs in cells isolated before or after the preovulatory LH surge[J].Mol Cell Endocrinol，2001，15：13-20..
- 9. Westergaard LG，Erb K，Laursen SB，et al.Concentrations of gonadotropins and steroids in preovulatory follicular fluid and serum in relation to stimulation protocol and outcome of assisted reproduction treatment[J].RBMonline，2004，8：516-523..
- 10. Slot KA，de BoerBrouwer M，Houweling M et al.Luteinizing hormone inhibits Fasinduced apoptosis in ovarian surface epithelial cell lines[J].J Endocrinol，2006，188：227-239.
- 11. Chen Q，Yano T，Matsumi H，et al.Crosstalk between Fas/Fas ligand system and nitric oxide in the pathway subserving granulosa cell apoptosis：a possible regulatory mechanism for ovarian atresia[J].Endocrinology，2005，146：808-815.
