Effect of Listeria DNA on Expression of Bcl-2 in H22 Hepatocarcinoma in Mice_West China Medical Journal

Authors：

SHI Zhongrui ¹ , WANG Wei ² ,  CAO Zhanliang ¹ , et al ³

1.Armed Police Medical College Hospital Laboratory;2.Armed Police Nutrition Branch Medical College Hospital;3.Laboratory Center College of the Armed Police，Tianjin 300162，China;

Corresponding author：

CAO Zhanliang, Email: caozhanliang0728@163.com

Keywords：

李斯特菌; DNA; H22肝癌; Bcl-2; RT-PCR

Video：

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目的：检测李斯特菌DNA对小鼠H22肝癌Bcl2基因表达的影响，探讨细菌DNA对肿瘤的抑制作用。方法：建立H22肝癌小鼠模型，从模型建立后的第一天开始，随机分组，分别给予李斯特菌DNA液（实验组）和生理盐水（对照组）。从癌组织中提取总RNA，设计目的基因引物和内参照GAPD引物。采用RT-PCR方法，分别检测实验组及对照组小鼠瘤体Bcl-2基因的表达，应用Bio-RAD凝胶成像系统及在Quantity One软件辅助下分析测定表达结果。结果：实验组小鼠瘤体Bcl-2表达低于对照组，经统计学处理，两者有显著性差异。结论：李斯特菌DNA对H-22肝癌小鼠肿瘤有抑制作用。

Citation： SHI Zhongrui,WANG Wei,CAO Zhanliang,et al. Effect of Listeria DNA on Expression of Bcl-2 in H22 Hepatocarcinoma in Mice. West China Medical Journal, 2009, 24(2): 380-382. doi: Copy

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1. Lin J，Lin E，Nemunaitis J.Bacteria in the treatment of cancer[J].Curr Opin Mol Ther，2004，6（6）：629-639..
2. Ryan RM，Green J，Lewis CE.Use of bacteria in anticancer therapies[J].Bioessays，2006，28（1）：84-94..
3. Tokunaga T，Yamamoto H，Shimada S，et al.Antitumor activity of deoxyribonucleic acid fraction from Mycobacterium bovis BCG.Ⅰ.Isolation，physicochemical characterization，and antitumor activity[J].J Natl Cancer Inst，1984，72（4）：955-962..
4. Ballas ZK，Rasmussen WL，Krieg AM.Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA[J].J Immunol，1996，157（5）：1840-1845..
5. Bauer M，Heeg K，Wagner H，et al.DNA activates human immune cells through a CpG sequencedependent manner[J].Immunology，1999，97（4）：699-705..
6. Krieg AM.CpG motifs in bacterial DNA and their immune effects[J].Annu Rev Immunol，2002，20：709-760..
7. Krieg AM，Wagner H.Causing a commotion in the blood：immunotherapy progresses from bacteria to bacterial DNA[J].Immunol Today，2000，21（10）：521-526..
8. Krieg AM，Yi AK，Matson S，et al.CpG motifs in bacterial DNA trigger direct Bcell activation[J].Nature，1995，374（6522）：546-549..
9. Paterson Y，Maciag PC.Listeriabased vaccines for cancer treatment[J].Curr Opin Mol Ther,2005,7（5）：454-460.
10. Reed JC.Bcl2 and the regulation of programmed cell death[J].J Cell Biol,1994,124（1-2）：16..
11. Oltvai ZN，Milliman CL，Korsmeyer SJ.Bcl2 heterodimerizes in vivo with a conserved homolog，Bax，that accelerates programmed cell death[J].Cell,1993,74（4）：609-619..
12. Fisher TC，Milner AE，Gregory CD，et al.bcl2 modulation of apoptosis induced by anticancer drugs：resistance to thymidylate stress is independent of classical resistance pathways[J].Cancer Res，1993，53（14）：3321-3326..
13. Sparwasser T，Koch ES，Vabulas RM，et al.Bacterial DNA and immunostimulatory CpG oligonucleotides trigger maturation and activation of murine dendritic cells[J].Eur J Immunol，1998，28（6）：2045-2054..
14. Stacey KJ，Sweet MJ，Hume DA.Macrophages ingest and are activated by bacterial DNA[J].J Immunol，1996，157（5）：2116-2122..
15. Cowdery JS，Chace JH，Yi AK，et al.Bacterial DNA induces NK cells to produce IFNγ in vivo and increases the toxicity of lipopolysaccharides[J].J Immunol，1996，156（12）：4570-4575..
16. Heckelsmiller K，Rall K，Beck S，et al.Peritumoral CpG DNA elicits a coordinated response of CD8 T cells and innate effectors to cure established tumors in a murine colon carcinoma model[J].J Immunol，2002，169（7）：3892-3899..
17. Yamamoto S，Yamamoto T，Shimada S，et al.DNA from bacteria，but not from vertebrates，induces interferons，activates natural killer cells and inhibits tumor growth[J].Microbiol Immunol，1992，36（9）：983-997..
18. Stacey KJ，Young GR，Clark F，et al.The molecular basis for the lack of immunostimulatory activity of vertebrate DNA[J].J Immunol，2003，170（7）：36143620..
19. Pamer EG.Immune responses to Listeria monocytogenes[J].Nat Rev Immunol，2004，4（10）：812823..
20. 戴智勤，朱为.细菌载体技术研究进展（上）[J].国外医学预防诊断治疗用生物制品分册，2005，28（1）：14-17.

West China Medical Journal

Effect of Listeria DNA on Expression of Bcl-2 in H22 Hepatocarcinoma in Mice

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