The Primary Study on Mechanism of the Effect of Tanshinone ⅡA on NB4 Cell-induced Procoagulant Activity in ECV304 Cells_West China Medical Journal

Authors：

DAI Yixia , YANG Yiming , MENG Wentong

Department of Hematology and Hematologic Research Institute,West China Hospital,Sichuan University,Chengdu 610041,China;

Corresponding author：

Keywords：

丹参酮ⅡA; 急性早幼粒细胞白血病; NB4细胞; ECV304细胞; 促凝活性; TNF-α

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目的:研究丹参酮ⅡA(Tan ⅡA)对急性早幼粒细胞白血病(APL)细胞株NB4细胞诱导的血管内皮细胞株(ECV304)促凝活性(PCA)的影响,并对其机制作初步探讨。方法:(1)分别用1.0μg/mL TanⅡA、0.3μg/mLATRA、0.01%DMSO、PRMI1640处理NB4细胞24、48和72h,取其上清液作为条件培养基(hNB4-CM)。将这些CM分别与ECV304细胞在37oC共同孵育0、4、8和12h,用反复冻融法制备ECV304细胞裂解液,采用一期凝血法测定其PCA;采用ELISA法测定条件培养基中的TNF-α 。(2)ECV304细胞与1.0μg/mL TanⅡA及TanⅡA 72h-NB4-CM 在37oC共同分别孵育6、12、24和48h,并以ATRA和DMSO分别作为阳性和阴性对照,用上述相同方法测定ECV304细胞裂解液的PCA。结果:(1)1.0 μg/mL Tan ⅡA可以诱导NB4细胞分化,其作用NB4细胞的培养基有一定的升高ECV304细胞PCA的作用,该作用在孵育4h时达高峰,之后ECV304细胞PCA逐渐下降。与0.3μg/mL ATRA的作用无统计学差异(P gt;0.05)。(2)1.0 μg/mL的TanⅡA对TanⅡA72h-NB4-CM促ECV304细胞PCA有抑制作用,其强度随作用时间增加而增加,与1.0μmol/L ATRA比较,P gt;0.05。(3)TanⅡA作用NB4细胞的培养基中TNF-α浓度,在作用前7h内随作用时间增加而增加,与0.3μg/mL ATRA比较无差异(P gt;0.05)。结论:Tan ⅡA能诱导NB4细胞分化,后者在分化过程中释放的TNF-α可能与ECV304细胞PCA活性升高有关;Tan-ⅡA又能抑制Tan-ⅡA-NB4-CM增强ECV304细胞PCA的作用。

Citation： DAI Yixia,YANG Yiming,MENG Wentong. The Primary Study on Mechanism of the Effect of Tanshinone ⅡA on NB4 Cell-induced Procoagulant Activity in ECV304 Cells. West China Medical Journal, 2009, 24(3): 654-657. doi: Copy

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West China Medical Journal

The Primary Study on Mechanism of the Effect of Tanshinone ⅡA on NB4 Cell-induced Procoagulant Activity in ECV304 Cells

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