Radiosensitivity of Lung Neoplasms by Liposome-mediated hPNAS-4 Gene, A Novel Apoptosis-related Human Gene_West China Medical Journal

Authors：

ZENG Hui ¹ , SHI Huashan ² ,  LI Zhiping ¹

1.Department of Abdominal Oncology, West China Hospital; 2.National Key Laboratory of Biotherapy and Cancer Center, West China Medical School, Sichuan University, Chengdu 610041, China;

Corresponding author：

LI Zhiping, Email: lizhiping620312@yahoo.com.cn

Keywords：

基因; hPNAS-4; Lewis肺癌; 放射治疗; 放疗敏感性

Video：

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目的:探讨人凋亡相关新基因PNAS-4(hPNAS-4)基因通过脂质体转染至Lewis肺癌LL2细胞后对放射治疗的增敏作用。〖HTH〗方法〖HTSS〗:用脂质体介导的转染技术,将hPNAS-4基因导入Lewis肺癌LL2细胞中,Western blot鉴定其过表达后,观察X射线照射对其集落形成的影响;流式细胞仪检测hPNAS-4基因或／和放疗(0,1,2,4,6 Gy)对LL2细胞生长抑制及凋亡的影响。〖HTH〗结果〖HTSS〗:通过Western blot证实了hPNAS-4基因在LL2细胞中的过表达。Lip-hPNAS-4加放射治疗处理组细胞的生存能力明显降低,肿瘤细胞的克隆形成明显减少,流式细胞术检测体外培养的肿瘤细胞凋亡明显增加。〖HTH〗结论〖HTSS〗:hPNAS-4基因联合放射治疗能产生协同抗肿瘤效应。

Citation： ZENG Hui,SHI Huashan,LI Zhiping,SHI Huashan,LI Zhiping. Radiosensitivity of Lung Neoplasms by Liposome-mediated hPNAS-4 Gene, A Novel Apoptosis-related Human Gene. West China Medical Journal, 2009, 24(4): 926-929. doi: Copy

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1. HUH J J, WOLF J K, FIGHTMASTER D L, et al. Transduction of adenovirus-mediated wild-type p53 after radiotherapy in human cervical cancer cells[J]. Gynecol Oncol,2003,89:243-250..
2. SAH N K, MUNSHI A, NISHIKAWA T, et al. Adenovirus-mediated wild-type p53 radiasensitizes human tumor cells by suppressing DNA repair capacity[J]. Mol Cancer Ther,2003,2:1223-1231.
3. LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
4. STRAUBERG R L, FEINGOLD E A, GROUSE L H, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA,2002,99(26):16899-16903..
5. STRAUBERG R L, FEINGOLD E A, GROUSE L H, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA,2002,99(26):16899-16903..
6. FILIPPOV V, FILIPPOVA M, SINHA D, et al. PNAS-4: a novel pro-apoptotic gene activated during the early response to DNA damage[J]. Proc Am Assoc Cancer Res,2005,46:717(Abstr.3050)..
7. FILIPPOV V, FILIPPOVA M, SINHA D, et al. PNAS-4: a novel pro-apoptotic gene activated during the early response to DNA damage[J]. Proc Am Assoc Cancer Res,2005,46:717(Abstr.3050)..
8. YANG F(杨帆), LI Z Y, DENG H X, et al. Efficient inhibition of ovarian cancer growth and prolonged survival by transfection with a novel pro-apoptotic gene, hPNAS-4, in a mouse model. In vivo and in vitro results[J]. Oncology,2008,75(3-4):137-144..
9. YANG F(杨帆), LI Z Y, DENG H X, et al. Efficient inhibition of ovarian cancer growth and prolonged survival by transfection with a novel pro-apoptotic gene, hPNAS-4, in a mouse model. In vivo and in vitro results[J]. Oncology,2008,75(3-4):137-144..
10. WEI Y Q, ZHAO X, KARIYA Y, et al. Induction of apoptosis by quercetin: involvement of heat shock protein[J]. Cancer Res,1994,54:4952-4957..
11. WEI Y Q, ZHAO X, KARIYA Y, et al. Induction of apoptosis by quercetin: involvement of heat shock protein[J]. Cancer Res,1994,54:4952-4957..
12. MAUCERI H J, SEUNG L P, GRDINA W L, et al. Increased injection number enhances adenoviral genetic radiotherapy[J]. Radiat Oncol Invest,1997,5(5):220-226..
13. MAUCERI H J, SEUNG L P, GRDINA W L, et al. Increased injection number enhances adenoviral genetic radiotherapy[J]. Radiat Oncol Invest,1997,5(5):220-226..
14. NAKANO K, VOUSDEN K H. PUMA, a novel proapoptotic gene, is induced by P53[J]. Mol Cell,2001,7:683-694.
15. NAKANO K, VOUSDEN K H. PUMA, a novel proapoptotic gene, is induced by P53[J]. Mol Cell,2001,7:683-694..
16. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
17. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
18. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
19. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
20. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
21. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
22. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.[3]LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
23. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.

West China Medical Journal

Radiosensitivity of Lung Neoplasms by Liposome-mediated hPNAS-4 Gene, A Novel Apoptosis-related Human Gene

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