目的:探讨丙型肝炎病毒非结构蛋白NS4B在原发性肝癌发生中的作用,及其发生机制。方法:设置对照组、空白载体PCXN2组、转染NS4B组。使用脂质体介导法,转染丙型肝炎病毒非结构蛋白重组质粒NS4B进入Chang肝细胞内,并用G418筛选。绘制生长曲线,分别用流式细胞仪检测瞬时表达及稳定表达时肝细胞凋亡率,用均数±标准差表示,统计学分析采用Dunnett t检验及q检验。结果:瞬时表达各组(PCXN2,NS4B)凋亡率分别为:(918±060)%,(445±053)%。稳定表达各组(PCXN2,NS4B)凋亡率分别为:(1575±209)%,(366±034)%。与PCXN2组比较P lt;001。结论:NS4B抑制肝细胞凋亡率,可能导致肝细胞异常增殖,诱导肝癌发生。
Citation：
XU Jianyu,LI Changping,CHEN Feng,et al. Effect of NonStructural Protein 4B in Hepatitis C Virus on Apoptosis in Hepatic Cells. West China Medical Journal, 2009, 24(5): 1165-1167. doi:
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| 1. |
FUKUZAWA K, TAKAHASHI K, FURUTA K, et al. Expression of fas/fas ligaand (fasL) and its involvement in infiltrating lymphocytes in hepatocellular carcinoma(HCC)[J]. J Gastroenterol,2001,36(10):727728.
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| 2. |
刘重阳,刘为纹,陈东风,等.丙型肝炎病毒核心蛋白对细胞凋亡的影响[J].世界华人消化杂志,2001,9(10):11251128.
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| 3. |
PARK JS, YANG JM, MIN MK. Hepatitis C virus nonstructural protein NS4B transforms NIH3T3 cells in cooperation with the Haras oncogene[J]. Biochem Biophys Res Commun,2000,267(2):581587.
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- 1. FUKUZAWA K, TAKAHASHI K, FURUTA K, et al. Expression of fas/fas ligaand (fasL) and its involvement in infiltrating lymphocytes in hepatocellular carcinoma(HCC)[J]. J Gastroenterol,2001,36(10):727728.
- 2. 刘重阳,刘为纹,陈东风,等.丙型肝炎病毒核心蛋白对细胞凋亡的影响[J].世界华人消化杂志,2001,9(10):11251128.
- 3. PARK JS, YANG JM, MIN MK. Hepatitis C virus nonstructural protein NS4B transforms NIH3T3 cells in cooperation with the Haras oncogene[J]. Biochem Biophys Res Commun,2000,267(2):581587.
