Experimental Observation of Alcohol Extractive of Folium Eucommiae on the Rats with Hyperlipidemia Fatty Liver_West China Medical Journal

Authors：

JIANG Yuanming ¹ , LIU Yingju ² , WANG Menghua ² , et al. ²

1Suining Center Hospital, Suining Sichuan 629000; 2Department of Pharmacology, Chongqing University of Medical Sciences, Chongqing 400016, China;

Corresponding author：

Keywords：

杜仲叶; 脂肪肝; 氧化应激; 肿瘤坏死因子α

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摘要:目的:通过建立高脂血症脂肪肝动物模型,探讨杜仲叶醇提取物(alcohol extractive of Folium Eucommiae,AEFE)对脂肪肝的防治作用。方法:高脂饮食8周加维生素D36×105IU/kg分3d腹腔注射，建立大鼠高脂血症脂肪肝动物模型。各给药组从造模第3周开始分别灌胃给予洛伐他汀(4mg·kg-1·d-1)、 AEFE(70、140和420mg·kg-1·d-1),共给药6周。各组每天灌服等量生理盐水，灌胃容积为0.5mL/100g体重。结果:AEFE用药6周后显示,各剂量组血清胆固醇、甘油三酯和游离脂肪酸、谷丙转氨酶和谷草转氨酶均较模型组明显降低(P lt;005),肝组织丙二醛含量降低和超氧化物歧化酶活性升高,肝脂肪变性和炎症反应减轻,尤以中剂量组作用最明显(P lt;001)。AEFE对肝内肿瘤坏死因子α浓度无明显影响。结论:AEFE改善高脂性大鼠肝脏脂肪变性,此作用可能与其降血脂、抗氧化损伤有关。

Citation： JIANG Yuanming,LIU Yingju,WANG Menghua,et al.. Experimental Observation of Alcohol Extractive of Folium Eucommiae on the Rats with Hyperlipidemia Fatty Liver. West China Medical Journal, 2009, 24(5): 1180-1182. doi: Copy

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1. 戚向阳,喻晓辉,张声华,等.杜仲的炮制及降压成分提取工艺的研究[J].中草药,1997,28(3):144147.
2. 杨鹏远,芮耀诚,焦亚斌.动脉粥样硬化大鼠实验模型的建立[J].第二军医大学学报,2003,24(7):802804.
3. 刘菲,钟岚,王军臣.茴三硫防治非酒精性脂肪性肝炎的实验研究[J].中华消化杂志,2002,22(9):532534.
4. SANYAL A J, CAMPBELLSARGENT C, MIRSHAHI F, et al. Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities[J]. Gastroenterology,2001,120(5):11831192.
5. FELDSTEIN A E, WERNEBURG N W, CANBAY A, et al. Free fatty acids promotes hepatic lipotoxicity by stimulating TNFα expression via a lysosomal pathway[J]. Hepatology,2004,40:185194.
6. 王本祥.现代中药药理与临床[M].天津:天津科技翻译出版公司,2004:679683.
7. 张康健,董娟娥,马柏林,等.杜仲次生代谢物部位差异性的研究[J].林业科学,2002,38(6):1216.
8. 王梦华,李恒华，刘颖菊,等.杜仲叶醇提取物对大鼠血管内皮细胞的保护作用[J].中国老年学杂志,2007,27(18):17661768.
9. 王梦华,刘颖菊,周歧新,等.杜仲叶乙醇提取物抗动脉粥样硬化作用的实验研究[J].中成药,2007,29(11):16871690.

West China Medical Journal

Experimental Observation of Alcohol Extractive of Folium Eucommiae on the Rats with Hyperlipidemia Fatty Liver

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