The Roles of Epigallocatechin3gallate in the Progression and Migration of Breast Cancer Cells_West China Medical Journal

Authors：

LIU Dongbo , WANG Ranran , LUO Ting

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

乳腺癌细胞; GRP78; EGCG; phosphop38MAPK

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目的:探讨表没食子儿茶素没食子酸酯(EGCG)对乳腺癌细胞MCF7生长的影响及对乳腺癌细胞MDAMB231迁移的影响。方法:MCF7细胞培养贴壁之后,加入EGCG处理,2d后收集蛋白,采用Western Blot检测磷酸化p38丝裂原活化蛋白激酶(phosphop38MAPK)的表达;同样处理后收集活细胞,用细胞计数法检测细胞的存活;取对数生长期的MDAMB231细胞,分至6孔板培养,使用EGCG处理后,采用细胞划线法探测乳腺癌细胞的迁移。结果:使用EGCG处理乳腺癌细胞后,phosphop38MAPK的表达降低,EGCG处理乳腺癌细胞4d后其增殖率降低50%,迁移活性降低。结论:EGCG处理乳腺癌细胞能抑制肿瘤细胞的生长以及迁移,这与p38MAPK信号通路相关。

Citation： LIU Dongbo,WANG Ranran,LUO Ting. The Roles of Epigallocatechin3gallate in the Progression and Migration of Breast Cancer Cells. West China Medical Journal, 2009, 24(5): 1185-1188. doi: Copy

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10.	谭晓华,周殿元,张亚历,等.没食子儿茶素没食子酸酯诱导大肠癌LoVo细胞凋亡和凋亡相关基因表达的改变[J].中华医学杂志,1999,79(4):308310.
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1. 朱发伟.茶叶中表没食子儿茶素没食子酸酯的研究进展[J].中草药,2005,36(8):12711171---1272.
2. 李奕.表没食子儿茶素没食子酸酯抗白血病的分子机制[J].国外医学医学地理分册,2004,25(4):178181.
3. SAEKI K, KOBAYASHI N, INAZAWA Y, et al. Oxidationtriggered cJun Nterminal kinase (JNK) and p38 mitogenactivated protein (MAP) kinase pathways for apoptosis in human leukaemic cells stimulated by epigallocatechin3gallate (EGCG): a distinct pathway from those of chemically inducedand receptormediated apoptosis [J]. Biochem J,2002,368(3):705720.
4. HUANG S, NEW L, PAN Z X, et al. Urokinase plasminogen activator/urokinase specific surface receptor expression and matrix invasion by breast cancer cells requires constitutive p38 mitogenactivated protein kinase activity[J]. J Biol Chem,2000,275(16):1226612272.
5. 李鹤成,陆劲松,侯意枫,等.EGCG对人乳腺癌MDAMB435细胞株细胞增殖的抑制及机制[J].中国癌症杂志,2004,14(1):911.
6. LEA M A, XIAO Q, SADHUKHAN A K, et al. Inhibitory action of tea extracts and()epigallocatechin gallate on cell proliferation[J]. Proc Annu Meet Am Assoc Cancer Res,1993,34(4):771.
7. HASTAK K, GUPTA S, AHMAD N, et al. Role of p53 and NFκB in epigallocatechin3gallateinduced apoptosis of LNCaP cells[J]. Oncogene,2003,22(31):48514859.
8. 余艳辉,丁仁奎,张志伟,等.酯型儿茶素单体EGCG和GCG对人肠癌SW480细胞生长抑制作用及其机制[J].中国医师杂志,2005,7(2):161163.
9. 谭晓华,张亚历,周殿元.EGCG诱导胃癌和肝癌细胞凋亡及bcl2表达下调的研究[J].癌症,2000,19(17):638641.
10. 谭晓华,周殿元,张亚历,等.没食子儿茶素没食子酸酯诱导大肠癌LoVo细胞凋亡和凋亡相关基因表达的改变[J].中华医学杂志,1999,79(4):308310.
11. CHUNG J Y, PARK J O, PHYU H P, et al. Mechanisms of inhibition of the rasMAP kinase signaling pathway in 307b ras 12 cells by tea polyphenols()epigallocatechin3gallate and theaflavin3,3′digallate[J]. FASEB J,2001,15(11):20222024..
12. CHOI Y J, LIM S Y, WOO J H, et al. Sodium orthovanadate potentiates EGCGinduced apoptosis that is dependent on the Erk pathway[J]. Biochem Biophys Res Commun,2003,305(1):176185.

West China Medical Journal

The Roles of Epigallocatechin3gallate in the Progression and Migration of Breast Cancer Cells

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