• 1.the Department of Cardiovascular Surgery, Beijing University Shenzhen Hospital, Shenzhen Guangdong 518036, China; 2.the Department of Pediatrics, Research Centre of CHU SainteJustine, University of Montreal, Montreal, QC. Canada, H3T 1C5; 3.the Department of Biophotonics, International Laser Centre, Bratislava, Slovakia 81219;
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目的:应用心肌自发荧光(AF)研究心肌线粒体氧化代谢状态,监测线粒体功能改变的早期信号。方法:烟酰胺腺嘌呤(磷酸)二核苷酸[NAD(P)H]作为荧光探针,用光谱分辨的时间相关单光子计数(TCSPC)记录375nm紫外激光激发的心肌AF光谱和荧光寿命,测试影响线粒体呼吸时AF动态衰减。结果:在420~560nm光谱区域,至少需用3个荧光寿命池0.4~0.7ns,1.2~1.9ns和8.0~13.0ns描述细胞AF。线粒体呼吸阻断剂鱼藤酮可显著增加AF强度,缩短平均荧光寿命。氧化磷酸化解偶联剂二硝基酚可显著降低AF强度,在520nm处增宽荧光光谱,延长平均荧光寿命。这些结果和NADH荧光动力学离体实验(in vitro)有可比性。结论:光谱分辨的荧光寿命技术测定心肌NAD(P)H荧光有很好的重复性,在细胞水平上增加了心肌氧化代谢或线粒体功能障碍的知识,为临床诊断和治疗线粒体功能障碍开拓了新视野。

Citation: CHENG Ying,REN Mingming,LIU Yunqi,et al.. Assessment of Mitochondrial Metabolic Oxidative State in Living Cardiomyocytes with Cardiomyocyte Autofluorescence. West China Medical Journal, 2009, 24(7): 1767-1771. doi: Copy