Effect of Ecdysterone on Protein Expression of IRS1/2 in Insulinresistant HepG2 Cells_West China Medical Journal

Authors：

1.the Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou Sichuan 646000, China; 2. the people’s Hospital of Ziyang city, Ziyang Sichuan 641300;3. the Department of Pharmacology, Chonyqing Medical University, Chongqing 400016, China;

Corresponding author：

Keywords：

蜕皮甾酮; 胰岛素抵抗; 细胞模型; 胰岛素受体底物

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目的:探讨蜕皮甾酮对胰岛素抵抗(IR)HepG2细胞胰岛素受体底物(IRS)蛋白表达的影响。方法:胰岛素抵抗HepG2细胞模型建立后,培养液中加入蜕皮甾酮共同孵育,观察蜕皮甾酮及吡格列酮对模型细胞葡萄糖掺入率的影响;应用免疫细胞化学染色法等方法观察蜕皮甾酮对胰岛素抵抗HepG2细胞IRS-1、IRS-2表达的影响。结果:与模型细胞组比较,1×10-5mol/L蜕皮甾酮可使IR HepG2细胞IRS-1、IRS-2蛋白的表达显著增加。结论:蜕皮甾酮的胰岛素增敏作用可能与胰岛素信号转导分子IRS-1、IRS-2蛋白的表达增强有关。

Citation： CHEN Qiu,ZHU Yuxia,Liu Xuepeng,et al.. Effect of Ecdysterone on Protein Expression of IRS1/2 in Insulinresistant HepG2 Cells. West China Medical Journal, 2009, 24(7): 1772-1775. doi: Copy

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11.	DONG X, PARK S, LIN X, et al. Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth[J]. J Clin Invest,2006,116(1):101-114..
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1. DINAN L, LAFONT R. Effects and applications of arthropod steroid hormones (ecdysteroids) in mammals[J]. J Endocrinol,2006,191(1):1-8..
2. 陈秋,夏永鹏,邱宗荫.蜕皮甾酮对胰岛素抵抗细胞模型胰岛素敏感性和糖代谢的影响[J].中国药理学通报,2006,22(4):460-464..
3. 陈秋,邹德平,张蓉,等.蜕皮甾酮对胰岛素抵抗HepG2细胞胰岛素受体表达的影响[J].山东医药,2008,48(4):13-15..
4. 陈秋,夏永鹏,邱宗荫.吡格列酮对HepG2胰岛素抵抗细胞模型的药理学评价[J].中国药理学通报,2006,22(2):248-251..
5. THIRONE A C, HUANG C, KLIP A. Tissuespecific roles of IRS proteins in insulin signaling and glucose transport Ⅰ[J]. Trends Endocrinol Metab,2006,17(2):77-78..
6. EGAWA K, NAKASHIMA N, SHARMA P M, et al. Persistent activation of phosphatidylinositol 3kinase causes insulin resistance due to accelerated insulin induced insulin receptor substrate1 degradation in 3T3L1 adipocytes[J]. Endocrinology,2000,141:1930-1935..
7. ZHANDE R, MITCHELL J J, WU J, et al. Molecular mechanism of insulininduced degradation of insulin receptor substrate 1[J]. Mol Cell Biol,2002,2:1016-1026..
8. PEDERSON T M, KRAMER D L, RONDINONE C M. Serine/threonine phosphorylation of IRS1 triggers its degradation:possible regulation by tyrosine phosphorylation[J]. Diabetes,2001,50:24-31..
9. AGUIRRE V, WERNER E D, GIRAUD J, et al. Phosphorylation of Ser307 in insulin receptor substrate1 blocks interactions with the insulin receptor and inhibits insulin action[J]. J Biol Chem,2002,277:1531-1537.
10. YUAN L, ZIEGLER R, HAMANM A. Inhibition of the phosphoenolpyruvate carboxykinase gene expression by metformin in cultured hepatocytes[J]. Chin Med J,2002,115:1843-1848..
11. DONG X, PARK S, LIN X, et al. Irs1 and Irs2 signaling is essential for hepatic glucose homeostasis and systemic growth[J]. J Clin Invest,2006,116(1):101-114..
12. CHEN Q, XIA Y P, QIU Z Y. Effect of ecdysterone on glucose metabolism in vitro[J]. Life Sciences,2006,78(10):1108-1113..

West China Medical Journal

Effect of Ecdysterone on Protein Expression of IRS1/2 in Insulinresistant HepG2 Cells

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