Effects of High Fat and Salt Diet and Aerobic Exercise on the Expression of PPARs in the Rat Kidney_West China Medical Journal

Authors：

SHEN Chengyi ¹ , YAN Zhencheng ² , ZHU Zhiming. ²

1.Pathophysiological Research Laboratory, North Sichuan Medical College, Nanchong Sichuan 637007; 2.Center for Hypertension and Metabolic Diseases, the Third Military Medical University, Chongqing 400042, China;

Corresponding author：

Keywords：

大鼠; 代谢综合征; 有氧运动; 肾脏; PPARs

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目的:检测高脂高盐饮食和有氧运动对肾脏过氧化物酶体增殖物激活受体(PPARs)蛋白表达的影响。方法:20只8周龄雄性Wistar大鼠,分别给予普通膳食(RN)、高脂高盐膳食(HN)、普通膳食加游泳(RS)和高脂高盐膳食加游泳(HS)处理(每组5只),24周后采用Western blot方法检测各组大鼠肾脏组织的3种PPARs亚型(α、β/δ和γ)蛋白的表达量。结果:3种PPARs亚型在大鼠肾脏组织中均有表达。HN、RS和HS组PPAR-α蛋白表达量分别为RN组的4.0、3.0和2.1倍,PPARβ/δ蛋白表达量分别为RN组(对照)的3.0、2.5和4.0倍,PPAR-γ蛋白表达量为RN组的1.7、3.1和4.1倍,每个亚型的蛋白表达量在各组之间(两两比较)均有显著性差异。结论:高脂高盐膳食和有氧运动均可导致大鼠肾组织内3种PPARs亚型蛋白表达显著升高,有氧运动显著降低了高脂高盐膳食引起的PPAR-α蛋白高表达。

Citation： SHEN Chengyi,YAN Zhencheng,ZHU Zhiming.. Effects of High Fat and Salt Diet and Aerobic Exercise on the Expression of PPARs in the Rat Kidney. West China Medical Journal, 2009, 24(7): 1776-1778. doi: Copy

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1. RUAN X, ZHENG F, GUAN Y. PPARs and the kidney in metabolic syndrome[J]. Am J Physiol Renal Physiol,2008,294(5):F1032-1047..
2. GUAN Y. Peroxisome proliferatoractivated receptor family and its Relationship to renal complications of the metabolic syndrome[J]. J Am Soc Nephrol,2008,15(11):2801-2815..
3. 沈成义,闰振成,祝之明．Wistar大鼠代谢综合征动物模型的构建及其特征分析[J]．川北医学院学报,2007,22(6):526-529..
4. 沈成义,闫振成,祝之明．有氧运动防治代谢综合征的实验研究[J]．中华老年多器官疾病杂志,2008,7(1):43-46..
5. 张刚,刘道燕,祝之明.代谢综合征的危险因素[A]．见:祝之明．代谢综合征:病因探索与临床实践[M].北京:人民军事出版社,2005:32-49..
6. FINCK B N, HAN X, COURTOIS M, et al. A critical role for PPAR αmediated lipotoxicity in the pathogenesis of diabetic cardiomyopathy:modulation by dietary fat content[J]. Proc Natl Acad Sci USA,2003,100(3):1226-1231..
7. CHA D R, HAN J Y, SU D M, et al. Peroxisome proliferatoractivated receptorα deficiency protects aged mice from insulin resistance induced by highfat diet[J]. Am J Nephrol,2007,27(5):479-482..
8. MISHRA R, EMANCIPATOR S N, MILLER C, et al. Adipose differentiationrelated protein and regulators of lipid homeostasis identified by gene expression profiling in the murine db/db diabetic kidney[J]. Am J Physiol Renal Physiol,2004,286(5):F913-F921..
9. LEE C H, CHAWLA A, URBIZTONDO N, et al. Transcriptional repression of at herogenic inflammation modulation by PPARδ[J]． Science,2003,302(2644):453-457..
10. MOILER D E． New drug targets for type 2 diabetes and the metabolic syndrome[J]．Nature,2001,414(6865):821-827..
11. MCCALL G E, BYRNES WC, DICKINSON A, et al. Muscle fiber hypertrophy, hyperplasia, and capillary density in college men after resistance training[J]. J Appl Physiol,1996,81(5):2004-2012..
12. LUQUET S, LOPEZSORIANO J, HOLST D, et al. Peroxisome proliferatoractivated receptor δ controls muscle development and oxidative capability[J]. FASEB J,2003,17(15):2299-2301..
13. KAWAMURA T, YOSHIDA K, SUGAWARA A, et al. Regulation of skeletal muscle peroxisome proliferatoractivated receptor γ expression by exercise and angiotensinconverting enzyme inhibition in fructosefed hypertensive rats[J]. Hypertens Res,2004,27(1):61-70..
14. TUNSTALL R J, MEHAN K A, WADLEY G D, et al. Exercise training increases lipid metabolism gene expression in human skeletal muscle[J]. Am J Physiol Endocrinol Metab,2002,283(1):E66-72..
15. KANNISTO K, CHIBALIN A, GLINGHAMMAR B, et al. Differential expression of peroxisomal proliferator activated receptors α and δ in skeletal muscle in response to changes in diet and exercise[J]. Int J Mol Med,2006,17(1):45-52.

West China Medical Journal

Effects of High Fat and Salt Diet and Aerobic Exercise on the Expression of PPARs in the Rat Kidney

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