目的:探讨液相芯片检测胰腺炎患者外周血中细胞因子浓度的可行性,及其在胰腺炎中的临床应用价值。方法: 收集90例胰腺炎患者(包括重症和轻症)和30例正常对照者的外周血,离心提取血清,利用液相芯片检测血清中细胞因子IL10,IL6,TNFα的浓度,比较不同程度炎症患者以及与对照之间细胞因子的浓度差异,分析血清中细胞因子浓度差异与临床表现之间的关系。结果:液相芯片可以方便地在仅25 μl的血清样本中同时准确检测这三种细胞因子的含量,检测的有效范围为0.10～2000 pg/mL。IL10 的浓度在对照、轻型和重型胰腺炎组中分别为51.97±31.72 pg/mL, 32.88±9.70 pg/mL和3.57±0.99 pg/mL, 轻型组与重型组间以及重型组和对照间差异有显著性(P lt; 005)。IL10在发病三天内和三天后的浓度分别为 42.47±10.15 pg/mL和12.28±5.04 pg/mL, 两者间差异有显著性(P lt; 0.01). Il6 的浓度在三天后从60.90±24.37 pg/mL下降到34.52±13.57 pg/mL,但差异没有显著性(P gt;0.05);TNF-α的浓度在各组间没有显著差异(P gt;0.05)。结论:液相芯片检测技术可以快速准确的检测少量血样标本中IL10,IL-6,TNFα等细胞因子的浓度,在胰腺炎患者中细胞因子IL-10的浓度与胰腺炎轻重程度以及患病时间密切相关,IL-10的浓度可以作为该病临床处理的依据和药物疗效的监测指标。
Citation：
MA Jian,LIU Rong,ZHANG Rui.. Clinical Practice of Detecting Cytokines in Pancreatitis Patients’ Peripheral Blood by Using Liquichip. West China Medical Journal, 2009, 24(8): 1943-1945. doi:
Copy
Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
| 1. |
POORAN N, INDARAM A, SINGH P, et al. Cytokines (IL-6, IL-8, TNF): Early and Reliable Predictors of Severe Acute Pancreatitis[J]. Journal of Clinical Gastroenterology, 2003, 37(3): 263-266.
|
| 2. |
JAFFE H A, BUHL R, MASTRANGELI A, et al. Crystal Organ specific cytokine therapy. Local activation of mononuclear phagocytes by delivery of an aerosol of recombinant interferongamma to the human lung[J]. J Clin Invest, 1991, 88(1): 297–302.
|
| 3. |
TARANTINO G, COPPOLA A, CONCA P, et al. Serum TGFbeta 1 could be used to assess therapeutic outcome and shortterm prognosis of HCVrelated chronic hepatitis[J]. Int J Immunopathol Pharmacol, 2008, 21(4):1007-1012.
|
| 4. |
OSMAN MO, GESSER B, MORTENSEN JT, et al. Profiles of proinflammatory cytokines in the serum of rabbits after experimentally induced acute pancreatitis[J]. Cytokine, 2002, 17(1):53-59.
|
| 5. |
SALAMON M, SYSAJEDRZEJOWSKA A, LUKAMOWICZ J, et al. Concentration of selected cytokines in serum of patients with acne rosacea[J]. Przegl Lek, 2008, 65(9):371-374.
|
| 6. |
SHARMA A, SATYAM A, SHARMA JB. Leptin, IL10 and inflammatory markers (TNFalpha, IL-6 and IL-8) in preeclamptic, normotensive pregnant and healthy nonpregnant women[J]. Am J Reprod Immunol, 2007, 58(1):21-30.
|
- 1. POORAN N, INDARAM A, SINGH P, et al. Cytokines (IL-6, IL-8, TNF): Early and Reliable Predictors of Severe Acute Pancreatitis[J]. Journal of Clinical Gastroenterology, 2003, 37(3): 263-266.
- 2. JAFFE H A, BUHL R, MASTRANGELI A, et al. Crystal Organ specific cytokine therapy. Local activation of mononuclear phagocytes by delivery of an aerosol of recombinant interferongamma to the human lung[J]. J Clin Invest, 1991, 88(1): 297–302.
- 3. TARANTINO G, COPPOLA A, CONCA P, et al. Serum TGFbeta 1 could be used to assess therapeutic outcome and shortterm prognosis of HCVrelated chronic hepatitis[J]. Int J Immunopathol Pharmacol, 2008, 21(4):1007-1012.
- 4. OSMAN MO, GESSER B, MORTENSEN JT, et al. Profiles of proinflammatory cytokines in the serum of rabbits after experimentally induced acute pancreatitis[J]. Cytokine, 2002, 17(1):53-59.
- 5. SALAMON M, SYSAJEDRZEJOWSKA A, LUKAMOWICZ J, et al. Concentration of selected cytokines in serum of patients with acne rosacea[J]. Przegl Lek, 2008, 65(9):371-374.
- 6. SHARMA A, SATYAM A, SHARMA JB. Leptin, IL10 and inflammatory markers (TNFalpha, IL-6 and IL-8) in preeclamptic, normotensive pregnant and healthy nonpregnant women[J]. Am J Reprod Immunol, 2007, 58(1):21-30.
