Effect of Resvratrol on the Expression of Caspase-3 in U251 Cell_West China Medical Journal

Authors：

CHEN Zhongyong ¹ , PENG Qiang. ²

1.The People’s Hospital of Dayi Country, Dayi Sichuan 611330; 2The Hospital Affiliated of Luzhou Medical College, Luzhou Sichuan 646000, China;

Corresponding author：

Keywords：

白藜芦醇; 脑胶质瘤; U251细胞; 细胞凋亡; caspase-3

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目的:探讨白藜芦醇对人脑胶质瘤U251细胞的凋亡相关因子caspase-3的作用。方法:应用RT-PCR方法和Western-blot分别检测凋亡因子caspase-3的RNA和蛋白水平的表达。结果:白藜芦醇能明显促进U251细胞的凋亡相关因子caspase-3的表达,存在剂量依赖与时间依赖性。结论:白藜芦醇能通过caspase－3途径诱导U251细胞的凋亡。

Citation： CHEN Zhongyong,PENG Qiang.. Effect of Resvratrol on the Expression of Caspase-3 in U251 Cell. West China Medical Journal, 2009, 24(8): 2139-2141. doi: Copy

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1. JANG M, CAI L, UDEANI G O, et al. Cancer chemopreventive activity of resveratro, a natural product derived from grapes[J]. Science,2003,275(5297):218-220.
2. OURIA M, GUKOVSKAYA A, JUNG Y, et al. Foodderived polyphenols inhibit pancrentic cancer growth through mitochondrial cytochrome C release and apoptosis[J]. Int J Cancer,2002,8(5):767-769.
3. ATHAR M, BACK JH, TANG X, et al. Resveratrol: a review of p re2clinical studies for human cancer prevention[J]. Toxicol Appl Pharmacol, 2007, 224 (3):274-283.
4. AZIOSNG, KRISHNAMOORTHY L, HARRISM, et al. Estrogen and resveratrol regulate Rac and Cdc42 signaling to the sctin cytoskeleton of metastatic breast cancer cells[J]. Neoplasia, 2007, 9 (2): 147-158.
5. FULDA S, DEBATIN KM. Resveratrolmediated sensitisation to TRAILinduced apoptosis depends on death receptor and mitochondrial signaling[J]. Fur J Cancer, 2005, 41 (5): 786-798.
6. RIGOLIO R, MILOSOM, NICOLINI G,et al. Resveratrol interference with the cell cycle protects human neuroblastoma SHSY5Y cell from paclitaxel induced apoptosis[J]. Neurochem Int, 2005, 46 (3): 205-211.
7. HORN TL, CWIK MJ, MORRISSEY RL, et al. Oncogenicity evaluation of resveratrol in p53(+ /) (p53 knock-out) mice[J]. Food Chem Toxicol, 2007, 45 (1):55-63.
8. 王玉, 孙黎光, 夏春辉. Caspase介导的Fas凋亡途径[J].世界华人消化杂志, 2006,14(36): 3439-3442.
9. 金琳芳,陈同钰.Bcl2基因蛋白家族与细胞凋亡[J]. 医学综述, 2005,11(5):446-447.

West China Medical Journal

Effect of Resvratrol on the Expression of Caspase-3 in U251 Cell

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