The Study of S100β Expression in Rat Astrocytes in vitro after Mechanical Injury_West China Medical Journal

Authors：

1.West China Preclinical Medicine and Forensic Medicine College, Sichuan University, Chengdu 610041; 2.Guangdong Nantian Expert Testimony Institute, Shenzhen Guangdong 518045; 3.Emeishan Public Security Bureau, Emeishan Sichuan 614200; 4.Guangdong Medical College, Dongguan Guangdong 523808, China;

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Keywords：

星形胶质细胞; 机械性损伤模型; S100β

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目的:观察体外培养星形胶质细胞损伤后S100β的表达变化,进一步了解脑损伤修复的分子机制,以期为法医病理学鉴定提供更多的依据。方法:取出生后24h内SD大鼠大脑皮质进行星形胶质细胞体外分离培养和纯化。随机分为对照组和损伤后30min、1h、3h、6h、12h、24h、3d、7d组,应用ABC法检测不同时间段S100β表达的差异。结果:体外培养星形胶质细胞的纯度达95％以上。对照组可见少量S100β蛋白表达;损伤后30min,S100β开始增加,3~6h达高峰,随后下降,24h时低于对照组水平,此后略有上升,7d时恢复至对照组水平。结论: 机械性划痕损伤后S100β总体表达呈单峰,而在体动物实验结果表明大鼠大脑损伤后S100β总体表达成双峰趋势,但均具有时序性变化规律,说明损伤后S100β表达可为脑损伤时间的推断依据之一;体外培养细胞损伤模型对组织细胞损伤的分子机制研究具有优越性。

Citation： HU Ling,YU Binhua,CHEN Xiaogang,et al.. The Study of S100β Expression in Rat Astrocytes in vitro after Mechanical Injury. West China Medical Journal, 2009, 24(9): 2367-2369. doi: Copy

1.	薛庆善,肖渝平.神经胶质细胞的体外培养方法[J].解剖科学进展, 1999,5(2):113-117..
2.	MCCARTHY K D, VELLIS J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue [J]. Cell Biol,1980,85(3):890-902..
3.	HILDEBRAND B, OLENIK C, MEYER D K. Neurons are generated in confluent astroglial cultures of rat neonatal neocortex [J]. Neuroscience,1997,78(4):957-966..
4.	TOMITA A, YOSHIDAS S, YANO M, et al. Removal after addition of NOgenerating agents and 8bromocyclic GMP causes morphological change of cultured cerebellar astrocytes:a new mode of NO action [J]. Brain Res,1997,774(2):344-346..
5.	BACCI A, VERDERIO C, PRAVETTONI E, et al. The role of glial cells in synaptic function.Philos Trans R Soc Lond [B]. Biol Sci,1999,354 (1381):403-409..
6.	MAUCH D H, NAGLER K, PRIEGER F W, et al. CNS synaptogenesis promoted by gliaderived cholesterol [J].Science,2001,294(5545):1354-1357..
7.	PELINKA L E, TOEGEL E, MAURITZ W, et al. Serum S100β:a marker of brain damage in traumatic brain injury with and without multiple trauma [J].Shock,2003,19(3):195-200..
8.	黄平,刘岩峰,托娅等.大鼠弥漫性脑损伤后S100β表达与损伤时间关系 [J]. 法医学杂志.2006,1(22):4-6..

1. 薛庆善,肖渝平.神经胶质细胞的体外培养方法[J].解剖科学进展, 1999,5(2):113-117..
2. MCCARTHY K D, VELLIS J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue [J]. Cell Biol,1980,85(3):890-902..
3. HILDEBRAND B, OLENIK C, MEYER D K. Neurons are generated in confluent astroglial cultures of rat neonatal neocortex [J]. Neuroscience,1997,78(4):957-966..
4. TOMITA A, YOSHIDAS S, YANO M, et al. Removal after addition of NOgenerating agents and 8bromocyclic GMP causes morphological change of cultured cerebellar astrocytes:a new mode of NO action [J]. Brain Res,1997,774(2):344-346..
5. BACCI A, VERDERIO C, PRAVETTONI E, et al. The role of glial cells in synaptic function.Philos Trans R Soc Lond [B]. Biol Sci,1999,354 (1381):403-409..
6. MAUCH D H, NAGLER K, PRIEGER F W, et al. CNS synaptogenesis promoted by gliaderived cholesterol [J].Science,2001,294(5545):1354-1357..
7. PELINKA L E, TOEGEL E, MAURITZ W, et al. Serum S100β:a marker of brain damage in traumatic brain injury with and without multiple trauma [J].Shock,2003,19(3):195-200..
8. 黄平,刘岩峰,托娅等.大鼠弥漫性脑损伤后S100β表达与损伤时间关系 [J]. 法医学杂志.2006,1(22):4-6..

West China Medical Journal

The Study of S100β Expression in Rat Astrocytes in vitro after Mechanical Injury

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