Effects of X-Ray Dose on Expressions of MicroRNA-221 and PTEN in Human Colorectal Carcinoma Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

HONG Yubo ¹ , SUN Kai ² , ZHANG Xiaobin ² , LEI Shangtong ² ,  DENG Haijun ²

1.The Third Department of General Surgery， Affiliated Foshan Hospital， Southern Medical University， Foshan 528000， Guangdong Province， China;;
2.Department of General Surgery， Nanfang Hospital of Southern Medical University， Guangzhou 510515， Guangdong Province， China;

Corresponding author：

DENG Haijun, Email: navyd999@tom.com

Keywords：

Colorectal carcinoma; MicroRNA-221; PTEN; X-ray; Radiation dose; Radiosensitivity

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Objective 　To investigate the effects of X-ray dose on the expressions of microRNA-221 （miR-221） and phosphatase and a tensin homolog deleted from chromosome10 （PTEN） in human colorectal carcinoma （CRC） cells.
Methods 　Human CRC-derived cell line， Caco2， was cultured conventionally. The cells were divided into five groups and exposed to different doses of X-ray （0， 2， 4， 6， and 8 Gy） respectively. The total RNA and protein of the Caco2 cells were extracted after irradiation， and the miR-221 and PTEN mRNA expressions were detected by real-time RT-PCR.
Moreover， the protein alteration of PTEN in Caco2 cells was detected by Western-blot analysis.
Results 　The radiation dose of X-ray significantly affected the expressions of miR-221 and PTEN protein in human Caco2 cells in a dose-depen-dent manner. Moreover， the miR-221 expression level was up-regulated gradually with the increase of irradiation dose， on the contrary， the PTEN protein expression level was down-regulated gradually （P＜0.01）.
Conclusion 　The radiation dose can affect the miR-221 and PTEN protein expression pattern in CRC cells.

Citation： HONG Yubo,SUN Kai,ZHANG Xiaobin,LEI Shangtong,DENG Haijun. Effects of X-Ray Dose on Expressions of MicroRNA-221 and PTEN in Human Colorectal Carcinoma Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(10): 1113-1116. doi: Copy

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15.	陈荣，刘光辉，周总光，等. 外周血microRNA作为肿瘤标志物的研究进展[J]. 中国普外基础与临床杂志， 2012， 19(9)：1020-1023.
16.	孙凯，曾俊杰，王伟，等. 微小RNA-221 对结直肠癌中CDK-N1C/p57表达的调控研究[J]. 中华胃肠外科杂志， 2011， 14(4)：41-55.
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1. Jang MJ， Kim JW， Min KT， et al. Prognostic significance ofmicroRNA gene polymorphisms in patients with surgically resectedcolorectal cancer[J]. Exp Ther Med， 2011， 2(6)：1127-1132.
2. 李昂，曹锋，方育，等. 术前放化疗结合全直肠系膜切除在低位进展期直肠癌治疗中的应用[J]. 中国普外基础与临床杂志， 2012， 19(9)：971-974.
3. Sun K， Wang W， Zeng JJ， et al. MicroRNA-221 inhibits CDK-N1C/p57 expression in human colorectal carcinoma[J]. Acta Pharmacol Sin， 2011， 32(3)：375-384.
4. Leslie NR， Foti M. Non-genomic loss of PTEN function in cancer：not in my genes[J]. Trends Pharmacal Sci， 2011， 32(3)：131-140.
5. 王鹏，郭琳，王强，等. PTEN、Fas/FasL和MMP-2在胃癌组织的表达及其临床意义[J]. 中国普外基础与临床杂志， 2009， 16(12)：991-995.
6. Bunney TD， Katan M. Phosphoinositide signalling in cancer：beyond PI3K and PTEN[J]. Nat Rev Cancer， 2010， 10(5)：342- 352.
7. 巴一. 2012年NCCN指南结直肠癌新辅助及解救化疗方案变更与解读[J]. 中国实用外科杂志， 2012， 32(9)：716-719.
8. Ortholan C， Romestaing P， Chapet O， et al. Correlation in rectal cancer between clinical tumor response after neoadjuvant radiotherapy and sphincter or organ preservation：10-year results of the Lyon R 96-02 randomized trial[J]. Int J Radiat Oncol Biol Phys， 2012， 83(2)：165-171.
9. Bisceglia G， Mastrodonato N， Rucci B， et al. Effectiveness of neoadjuvant radiotherapy in the treatment of locally advanced rectal cancer：a single-center experience in 263 patients[J]. Dig Surg， 2010， 27(3)：217-223.
10. Lin C， Yu Y， Zhao HG， et al. Combination of quercetin with radiotherapy enhances tumor radiosensitivity in vitro and in vivo[J]. Radiother Oncol， 2012， 104(3)：395-400.
11. Schetter AJ， Okayama H， Harris CC. The role of microRNAs in colorectal cancer[J]. Cancer J， 2012， 18(3)：244-252.
12. Sun K， Guo C， Deng HJ， et al. Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure[J]. Acta Pharmacol Sin， 2013， 34(1)：167-175.
13. Sun K， Deng HJ， Lei ST， et al. miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened[J]. World J Gastroenterol， 2013， 19(14)：2197-2207.
14. Zhao L， Bode AM， Cao Y， et al. Regulatory mechanisms and clinical perspectives of miRNA in tumor radiosensitivity[J]. Carcinogenesis， 2012， 33(11)：2220-2227.
15. 陈荣，刘光辉，周总光，等. 外周血microRNA作为肿瘤标志物的研究进展[J]. 中国普外基础与临床杂志， 2012， 19(9)：1020-1023.
16. 孙凯，曾俊杰，王伟，等. 微小RNA-221 对结直肠癌中CDK-N1C/p57表达的调控研究[J]. 中华胃肠外科杂志， 2011， 14(4)：41-55.
17. Carracedo A， Alimonti A， Pandolfi PP. PTEN level in tumor suppression：how much is too little ?[J]. Cancer Res， 2011， 71(3)：629-633.
18. 肖华，张峰，王学浩，等. PTEN基因在过氧化物酶体增殖物激活受体γ调节肝癌细胞生长中的作用[J]. 中国普外基础与临床杂志， 2007， 14(4)：413-416.
19. Zhang C， Kang C， Wang P， et al. MicroRNA-221 and-222 regulate radiation sensitivity by targeting the PTEN pathway[J]. Int J Radiat Oncol Biol Phys， 2011， 80(1)：240-248.
20. 张晓槟，孙凯，雷尚通，等. Anti-microRNA-221通过上调PTEN蛋白表达增加结直肠癌细胞的放射增敏性[J]. 南方医科大学学报， 2013， 33(5)：728-732.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of X-Ray Dose on Expressions of MicroRNA-221 and PTEN in Human Colorectal Carcinoma Cells

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