Investigation of K-ras Gene Codon 12 and 13 Mutations in The Uyghur and Han Patients with Rectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

1. Xinjiang Clinical School of Anhui Medical University， Department of Anus and Intestine Surgery， People’s Hospital of Xinjiang Urgar Autonomous Region， Urumqi 830000， Xinjiang， China;;
2. Department of Anus and Intestine Surgery， People’s Hospital of Xinjiang Urgar Autonomous Region， Urumqi 830000， Xinjiang， China;;
3. Department of General Surgery， People’s Hospital of Xinjiang Urgar Autonomous Region，Urumqi 830000， Xinjiang， China;

Corresponding author：

Keywords：

Rectal cancer; K-ras gene; Gene mutation

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate K-ras gene exon 2 codon 12 and 13 mutations， and analyze the clinical signiﬁcance in the Uyghur and Han patients with rectal cancer.
Methods 　A total of 144 surgical specimens taken from patients with rectal cancer who were treated in this hospital from January 2010 to December 2011 were used in this study. DNA was picked up from the tumor tissues and amplificated by PCR then direct sequencing.
Results 　The K-ras gene muta-tion rate was 22.22% （32/144）， which was 26.09% （12/46） and 20.41% （20/98） in the Uyghur and Han patients， respec-tively， the difference was not statically significant （P＞0.05）. The K-ras gene mutation was related to the depth of invasion（T1＋T2：25.0%， T3＋T4：75.0%， P=0.01）， which was not related to the nation， gender， location of tumor， differen-tiation degree， or lymph node metastasis （P＞0.05）.
Conclusions 　K-ras gene mutation is a common event in the Uyghur and Han patients with rectal cancer， but the K-ras gene mutation rate is not signiﬁcant difference between the Uyghur nationality and Han nationality， which is only related to depth of invasion.

Citation： FU Dapeng,GONG Xuchen,KONG Chongqing.. Investigation of K-ras Gene Codon 12 and 13 Mutations in The Uyghur and Han Patients with Rectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(2): 165-168. doi: Copy

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12.	Ren J， Li G， Ge J， et al. Is K-ras gene mutation a prognostic factorfor colorectal cancer：a systematic review and meta-analysis[J]. Dis Colon Rectum， 2012， 55(8)：913-923.
13.	Zulhabri O， Rahman J， Ismail S， et al. Predominance of G to a codon 12 mutation k-ras gene in Dukes’ B colorectal cancer[J]. Singapore Med J， 2012， 53(1)：26-31.
14.	Sammoud S， Khiari M， Semeh A， et al. Relationship between expression of ras p21 oncoprotein and mutation status of the K-ras gene in sporadic colorectal cancer patients in Tunisia[J]. Appl Immunohistochem Mol Morphol， 2012， 20(2)：146-152.
15.	Beliaeva AV， Ianus GA， Suspithyn EN， et al. Age-related and clinical-pathogenetic features of colorectal cancer associated with status of K-ras gene[J]. Adv Gerontol， 2012， 25(1)：72-78.
16.	Yunxia Z， Jun C， Guanshan Z， et al. Mutations in epidermalgrowth factor receptor and K-ras in Chinese patients with colorectalcancer[J]. BMC Med Genet， 2010， 11：34. doi：10. 1186/1471-.
17.	Chang YS， Yeh KT， Chang TJ， et al. Fast simultaneous detectionof K-RAS mutations in colorectal cancer[J]. BMC Cancer， 2009，.
18.	Poehlmann A， Kuester D， Meyer F， et al. K-ras mutation detection in colorectal cancer using the pyrosequencing technique[J]. Pathol Res Pract， 2007， 203(7)：489-497.
19.	-11-34.

1. 刘伟，王丽，余英豪，等. k-ras基因在中国结直肠癌患者中的突变状态[J]. 世界华人消化杂志， 2011， 19(13)：1367-1374.
2. 高强，窦倩慧，方超，等，影响结肠癌患者术后并发症的多因素分析[J]. 中国普外基础与临床杂志， 2011， 18(2)：159-163.
3. Halatsch ME， Hirsch-Ernst KI， Weinel RJ， et al. Differentialactivation of the c-Ki-ras-2 proto-oncogene in human colorectal carcinoma[J]. Anticancer Research， 1998， 18(4A)：2323-2325.
4. Doolittle BR， Emanuel J， Tuttle C， et al. Detection of the mutatedK-Ras biomarker in colorectal carcinoma[J]. Exp Mol Pathol， 2001， 70(3)：289-301.
5. Wu CM， Tang R， Wang JY， et al. Frequency and spectrum of K-RAS codons 12 and 13 mutations in colorectal adenocarcinomas from Taiwan[J]. Cancer Genet Cytogenet， 2005， 158(1)：55-60.
6. Kinoshita H， Yanagisawa A， Watanabe T， et al. Increase in the frequency of K-ras codon 12 point mutation in colorectal carcinomain elderly males in Japan：the 1990s compared with the 1960s[J].Cancer Sci， 2005， 96(4)：218-220.
7. Naguib A， Mitrou PN， Gay LJ， et al. Dietary， lifestyle and clini-copathological factors associated with BRAF and K-ras mutationsarising in distinct subsets of colorectal cancers in the EPIC Norfolkstudy[J]. BMC Cancer， 2010， 10：99. doi：10. 1186/1471-2407-10-99.
8. Shen H， Yuan Y， Hu HG， et al. Clinical significance of K-ras and BRAF mutations in Chinese colorectal cancer patients[J]. World J Gastroenterol， 2011， 17(6)：809-816.
9. 袁瑛，胡涵光，叶晓贤，等. K-ras基因突变与结直肠癌临床病理因素的关系[J]. 中华外科杂志， 2010， 48(16)：1247-1251.
10. ：179. doi：10.1186/1471-2407-9-179.
11. Stec R， Bodnar L， Charkiewicz R， et al. K-Ras gene mutation status as a prognostic and predictive factor in patients with colorectal cancer undergoing irinotecan- or oxaliplatin-based chemotherapy[J]. Cancer Biol Ther， 2012， 13(13)：1235-1243.
12. Ren J， Li G， Ge J， et al. Is K-ras gene mutation a prognostic factorfor colorectal cancer：a systematic review and meta-analysis[J]. Dis Colon Rectum， 2012， 55(8)：913-923.
13. Zulhabri O， Rahman J， Ismail S， et al. Predominance of G to a codon 12 mutation k-ras gene in Dukes’ B colorectal cancer[J]. Singapore Med J， 2012， 53(1)：26-31.
14. Sammoud S， Khiari M， Semeh A， et al. Relationship between expression of ras p21 oncoprotein and mutation status of the K-ras gene in sporadic colorectal cancer patients in Tunisia[J]. Appl Immunohistochem Mol Morphol， 2012， 20(2)：146-152.
15. Beliaeva AV， Ianus GA， Suspithyn EN， et al. Age-related and clinical-pathogenetic features of colorectal cancer associated with status of K-ras gene[J]. Adv Gerontol， 2012， 25(1)：72-78.
16. Yunxia Z， Jun C， Guanshan Z， et al. Mutations in epidermalgrowth factor receptor and K-ras in Chinese patients with colorectalcancer[J]. BMC Med Genet， 2010， 11：34. doi：10. 1186/1471-.
17. Chang YS， Yeh KT， Chang TJ， et al. Fast simultaneous detectionof K-RAS mutations in colorectal cancer[J]. BMC Cancer， 2009，.
18. Poehlmann A， Kuester D， Meyer F， et al. K-ras mutation detection in colorectal cancer using the pyrosequencing technique[J]. Pathol Res Pract， 2007， 203(7)：489-497.
19. -11-34.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Investigation of K-ras Gene Codon 12 and 13 Mutations in The Uyghur and Han Patients with Rectal Cancer

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