Objective To observe the expression levels of nuclear factor kappa B (NF-κB), vascular endothelial growth factor (VEGF), and CD31 in portal vein and surrounding tissues of rats during the formation process of cavernous
transformation of portal vein (CTPV), and try to search the relationship between NF-κB, VEGF, and the angiogenesis
of portal areas, as well as the significance and the role of NF-κB and VEGF in the formation process of CTPV.
Methods One hundred and ten Sprague-Dawley (SD) rats were randomly (random number method) divided into sham operation group and model group. The partial constriction operations on portal vein were performed in model rats with a blunt 21G
caliber to establish CTPV animal models (model group), while the exploratory operations on portal vein, not constriction,
were performed in rats of sham operation group. All specimens (portal vein and surrounding tissues) were fixed in formalin
and made into paraffin blocks. Each specimen was tested by immunohistochemistry for the expressions of NF-κB, VEGF, and CD31, then optical density (OD) of NF-κB expression and the mean integral optical density (IOD) of VEGF expression
were measured by using Image Pro Plus 6.0 software, and microvessel density (MVD) was calculated under microscope.
Results Nucleoplasm ratio of OD value of NF-κB, mean IOD value of VEGF, and MVD value in 1, 2, 3, 4, and 6 weeks after operation didn’t significantly differed from that of before operation in sham operation group (P>0.05), but higher at all time points after operation in model group (P<0.01). Compared with sham operation group, nucleoplasm ratio of OD value of NF-κB, mean IOD value of VEGF, and MVD value were significantly higher in 1, 2, 3, 4, and 6 weeks after operation in model group (P<0.01). NF-κB and VEGF, NF-κB and MVD, VEGF and MVD were positively correlated with each other (r=0.654 6,P<0.01;r=0.620 7, P<0.01;r=0.636 9, P<0.01) in model group.
Conclusion NF-κB and VEGF may relate to the formation of CTPV, and may involve in the angiogenesis.
Citation:
MAO Jianxiong,LIU Lei,LI Suyi,WANG Bin,WANG Jianyao. Expressions of NF-κB and VEGF in The Formation of Cavernous Transformation of Portal Vein in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(3): 280-286. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. 王欣博, 童中勋, 刘威, 等. 门静脉海绵样变性外科治疗的研究进展[J]. 中国普外基础与临床杂志, 2008, 15(12):877-879.
- 2. 梁颖, 蒋涛, 王亚杰, 等. 螺旋CT评价门静脉海绵样变性及其侧支循环的特点[J]. 中国医学影像技术, 2008, 24(7):1076-1079.
- 3. 邓伟哲, 李柏. 门静脉高压侧支循环与血管内皮细胞生长因子[J]. 世界华人消化杂志, 2006, 14(16):1621-1626.
- 4. 方驰华, 项楠, 范应方, 等. 64层螺旋CT三维成像在消化系统疾病诊治中的临床应用价值[J]. 中华外科杂志, 2007, 45(13):909-912.
- 5. 王建尧, 刘磊, 王斌, 等. 门静脉海绵样变性大鼠模型制作方法的探讨及体会[J]. 中国普外基础与临床杂志, 2010, 17(1):46-51.
- 6. 张桂林, 刘尚喜, 邓燕, 等. 免疫化学染色图像分析法检测细胞核因子κB[J]. 第一军医大学学报, 2003, 23(5):498-500.
- 7. 旷野, 张忠林. 门静脉海绵样变性的多层螺旋CT诊断[J]. 南华大学学报:医学版, 2006, 34(4):541-544.
- 8. 刘于宝, 胡道予, 夏黎明, 等. 门静脉海绵样变性门脉胆支及胆系改变的MRI研究[J]. 中华肝胆外科杂志, 2005, 11(2):90-93.
- 9. Stumm M, Sieber C. Portal hypertension and angiogenesis[J]. Schweiz Med Wochenschr, 2000, 130(7):233-239.
- 10. Sen R, Baltimore D. Inducibility of κ immunoglobulin enhancer-binding protein NF-κB by a posttranslational mechanism[J]. Cell, 1986, 47(6):921-928.
- 11. Mörk H, Weber P, Schmidt H, et al. Cavernous transformation of the portal vein associated with common bile duct strictures:report of two cases[J]. Gastrointest Endosc, 1998, 47(1):79-83.
- 12. 毛建雄, 刘磊, 王斌, 等. 儿童门静脉海绵样变性脾静脉中NF-κB,VEGF的表达及意义[J]. 中国实用医药, 2012, 7(19):.
- 13. 张彤, 杨镇. 内皮型一氧化氮合酶、内皮素-1、蛋白激酶C、核因子-κB在门静脉高压血管内皮细胞的表达及意义[J]. 中华实验外科杂志, 2006, 23(11):1296-1298.
- 14. Moreira AJ, Fraga C, Alonso M, et al. Quercetin prevents oxida-tive stress and NF-κB activation in gastric mucosa of portal hypert-ensive rats[J]. Biochem Pharmacol, 2004, 68(10):1939-1946.
- 15. 李涛, 李海洋, 张彤, 等. 门脉高压症脾血管病变的研究[J]. 世界华人消化杂志, 2004, 12(7):1616-1622.
- 16. 王超, 韩娟, 刘中砚, 等. 核转录因子κB在门静脉高压大鼠肺血管中的表达及意义[J]. 微循环学杂志, 2005, 15(3):22-23.
- 17. Liu HL, Lo CR, Czaja MJ. NF-κB inhibition sensitizes hepatocytes to TNF-induced apoptosis through a sustained activation of JNK and c-Jun[J]. Hepatology, 2002, 35(4):772-778.
- 18. 钟霞, 于皆平, 冉宗学. 大肠癌组织中细胞核因子表达与肿瘤血管生成的关系[J]. 中国现代医学杂志, 2002, 12(16):6-9.
- 19. Kim H, Koh G. Lipopolysaccharide activates matrix metalloproteinase-2 in endothelial cells through an NF-kappaB-dependent pathway[J]. Biochem Biophys Res Commun, 2000, 269(2):401-405.
- 20. 刘宇, 张浩, 杨野, 等. 表皮生长因子介导的NF-κB促进胰腺癌细胞MMP-9表达及侵袭的实验研究[J]. 中国普外基础与临床杂志, 2009, 16(8):603-608.
- 21. Joyce D, Albanese C, Steer J, et al. NF-kappaB and cell-cycle regulation:the cyclin connection[J]. Cytokine Growth Factor Rev, 2001, 12(1):73-90.
- 22. 石力, 田伏洲, 李旭, 等. 急性胰腺炎大鼠肝脏NF-κB对TNF-α表达的调控及其在肝损伤中的作用[J]. 中国普外基础与临床杂志, 2004, 11(3):228-230.
- 23. Patel S, Leal AD, Gorski DH. The homeobox gene Gax inhibits angiogenesis through inhibition of nuclear factor-kappaB-dependent endothelial cell gene expression[J]. Cancer Res, 2005, 65(4):1414-1424.
- 24. -25.
- 25. Yoshida S, Ono M, Shono T, et al. Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis[J]. Mol Cell Biol, 1997, 17(7):4015-4023.
- 26. 史朝晖, 常新忠, 姜希宏, 等. 胃癌组织中核因子-κB p65的表达及其与血管内皮生长因子的关系[J]. 中国普外基础与临床杂志, 2004, 11(2):113-115.