Establishment of Duodenal-Jejunal Bypass Animal Model in Goto-Kakizaki Rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LIU Bin , YU Min , ZHANG Zaizhong , CHEN Xin , WANG Junjie , HUANG Sheng , WANG Lie , WANG Yu ,  WANG Bing

Department of General Surgery， Fuzhou General Hospital of Nanjing Military Command， Fuzhou 350025， Fujian Province， China;

Corresponding author：

WANG Bing, Email: wang213bing@163.com

Keywords：

Duodenal-jejunal bypass surgery; Goto-Kakizaki rat; Type 2 diabetes mellitus; Animal model

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Objective 　To explore the feasibility and operation points of establishing duodenal-jejunal bypass （DJB）
surgery animal model in Goto-Kakizaki （GK） rats.
Methods 　Sixteen GK rats were randomly divided into experimental group （n=8） and control group （n=8）. In a standardized preoperative， intraoperative， and postoperative operation， the rats of experimental group and control group received DJB and sham surgery respectively. The fasting plasma glucose and body mass were observed before operation， and 1， 2， 3， and 4 weeks after operation in order to evaluate whether the models were established successfully. Survival situation of rats were observed too.
Results 　All experimental rats survived at 4 weeks after the operation. Compared with the levels before operation， the fasting plasma glucose levels of experimental group decreased significantly （P＜0.05） at 1 week after operation， and remained stable at 2， 3， and 4 weeks after operation.
The fasting plasma glucose levels of control group did not change statistically at all time points after operation （P＞0.05）. Compared with control group at the same time point， the fasting plasma glucose level of experimental group was lower （P＜0.05）， indicating that DJB models were established successfully. After 4 weeks， the value of body mass added in experimental group was significantly lower than those of control group （P＜0.05）.
Conclusions 　DJB is a feasible， safe， and effective hypoglycemic surgery. The application of this set of experimental operating procedures can reduce the risk of intraoperative and postoperative mortality， and can develop a stable DJB model in Goto-Kakizaki rats.

Citation： LIU Bin,YU Min,ZHANG Zaizhong,CHEN Xin,WANG Junjie,HUANG Sheng,WANG Lie,WANG Yu,WANG Bing. Establishment of Duodenal-Jejunal Bypass Animal Model in Goto-Kakizaki Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(9): 1013-1017. doi: Copy

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8.	王瑜，王燕婷，王烈. 胃转流术对非肥胖型2型糖尿病的治疗作用[J]. 中国普通外科杂志， 2008， 17(10)：1003-1006.
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13.	Portha B， Giroix MH， Tourrel-Cuzin C， et al. The GK rat：a prototype for the study of non-overweight type 2 diabetes[J]. Methods Mol Biol， 2012， 933：125-159.
14.	Kimura K， Toyota T， Kakizaki M， et al. Impaired insulin secretion in the spontaneous diabetes rats[J]. Tohoku J Exp Med， 1982， 137(4)：453-539.
15.	de Luis D， Domingo M， Romero A， et al. Effects of duodenal-jejunal exclusion on beta cell function and hormonal regulation in Goto-Kakizaki rats[J]. Am J Surg， 2012， 204(2)：242-247.
16.	Bradley D， Conte C， Mittendorfer B， et al. Gastric bypass and banding equally improve insulin sensitivity and β cell function[J].J Clin Invest， 2012， 122(12)：4667-4674.
17.	Liu Y， Zhou Y， Wang Y， et al. Roux-en-Y gastric bypass-inducedimprovement of glucose tolerance and insulin resistance in type 2 diabetic rats are mediated by glucagon-like peptide-1[J]. Obes Surg， 2011， 21(9)：1424-1431.
18.	Zhang X， Huang W， Zhang Y， et al. Effects of Roux-en-Y operations on glucose homeostasis in obese GK rats[J]. Surg Endosc， 2011， 25(11)：3493-3498.
19.	Mueller RA， Lundberg DB， Breese GR， et al. The neuropharmacology of respiratory control[J]. Neuropharmacol Rev， 1982， 34(3)：255-285.
20.	Vila J. Protection from pentobarbital lethality mediated by pavlovianconditioning[J]. Pharmacol Biochem Behav， 1989， 32(1)：365-366.
21.	Han LO， Zhou LH， Cheng SJ， et al. Key details of the duodenal-jejunal bypass in type 2 diabetes mellitus rats[J]. World J Gastr-oenterol， 2011， 17(45)：5021-5027.
22.	Flegal MC， Kuhlman SM. Anesthesia monitoring equipment for laboratory animals[J]. Lab Anim (NY)， 2004， 33(7)：31-36.
23.	Lopez-Vidriero MT， Costello J， Clark TJ， et al. Effect of atropineon sputum production[J]. Thorax， 1975， 30(5)：543-547.
24.	Burch JM， Denton JR， Noble RD. Physiologic rationale for abbre-viated laparotomy[J]. Surg Clin North Am， 1997， 77(4)：779-782.
25.	Sessler DI. Mild perioperative hypothermia[J]. N Engl J Med， 1997， 336(24)：1730-1737.

1. Cummings DE， Flum DR. Gastrointestinal surgery as a treatment for diabetes[J]. JAMA， 2008， 299(3)：341-343.
2. Schauer PR， Ikramuddin S， Gourash W， et al. Outcomes after laparoscopic Roux-en-Y gastric bypass for morbid obesity[J]. Ann Surg， 2000， 232(4)：515-529.
3. Cummings DE， Overduin H， Foster-Schubert KE. Gastric bypass for obesity：mechanisms of weight loss and diabetes resolution[J].J Clin Endocrinol Metab， 2004， 89(6)：2608-2615.
4. Schauer PR， Burguera B， Ikramuddin S， et al. Effect of laparoscopic Roux-en-Y gastric bypass on type 2 diabetes mellitus[J]. Ann Surg， 2003， 238(4)：467-484.
5. Pories WJ. Diabetes：the evolution of a new paradigm[J]. Ann Surg， 2004， 239(1)：12-13.
6. Hickey MS， Pories WJ， MacDonald KG， et al. A new paradigm for type 2 diabetes mellitus：could it be a disease of the foregut?[J]. Ann Surg， 1998， 227(5)：637-643.
7. Rubino F， Marescaux J. Effect of duodenal-jejunal exclusion in a non-obese animal model of type 2 diabetes：a new perspective for an old disease[J]. Ann Surg， 2004， 239(1)：1-11.
8. 王瑜，王燕婷，王烈. 胃转流术对非肥胖型2型糖尿病的治疗作用[J]. 中国普通外科杂志， 2008， 17(10)：1003-1006.
9. Rubino F， Forgione A， Cummings DE， et al. The mechanism of diabetes control after gastrointestinal bypass surgery reveals a role of the proximal small intestine in the pathophysiology of type 2 diabetes[J]. Ann Surg， 2006， 244(5)：741-749.
10. Araujo AC， Bonfleur ML， Balbo SL， et al. Duodenal-jejunal bypass surgery enhances glucose tolerance and beta-cell function in western diet obese rats[J]. Obes Surg， 2012， 22(5)：819-826.
11. Klein S， Fabbrini E， Patterson BW， et al. Moderate effect of duod-enal-jejunal bypass surgery on glucose homeostasis in patients with type 2 diabetes[J]. Obesity (Silver Spring)， 2012， 20(6)：1266-1272.
12. Geloneze B， Geloneze SR， Chaim E， et al. Metabolic surgery fornon-obese type 2 diabetes：incretins， adipocytokines， and insulin secretion/resistance changes in a 1-year interventional clinical cont-rolled study[J]. Ann Surg， 2012， 256(1)：72-78.
13. Portha B， Giroix MH， Tourrel-Cuzin C， et al. The GK rat：a prototype for the study of non-overweight type 2 diabetes[J]. Methods Mol Biol， 2012， 933：125-159.
14. Kimura K， Toyota T， Kakizaki M， et al. Impaired insulin secretion in the spontaneous diabetes rats[J]. Tohoku J Exp Med， 1982， 137(4)：453-539.
15. de Luis D， Domingo M， Romero A， et al. Effects of duodenal-jejunal exclusion on beta cell function and hormonal regulation in Goto-Kakizaki rats[J]. Am J Surg， 2012， 204(2)：242-247.
16. Bradley D， Conte C， Mittendorfer B， et al. Gastric bypass and banding equally improve insulin sensitivity and β cell function[J].J Clin Invest， 2012， 122(12)：4667-4674.
17. Liu Y， Zhou Y， Wang Y， et al. Roux-en-Y gastric bypass-inducedimprovement of glucose tolerance and insulin resistance in type 2 diabetic rats are mediated by glucagon-like peptide-1[J]. Obes Surg， 2011， 21(9)：1424-1431.
18. Zhang X， Huang W， Zhang Y， et al. Effects of Roux-en-Y operations on glucose homeostasis in obese GK rats[J]. Surg Endosc， 2011， 25(11)：3493-3498.
19. Mueller RA， Lundberg DB， Breese GR， et al. The neuropharmacology of respiratory control[J]. Neuropharmacol Rev， 1982， 34(3)：255-285.
20. Vila J. Protection from pentobarbital lethality mediated by pavlovianconditioning[J]. Pharmacol Biochem Behav， 1989， 32(1)：365-366.
21. Han LO， Zhou LH， Cheng SJ， et al. Key details of the duodenal-jejunal bypass in type 2 diabetes mellitus rats[J]. World J Gastr-oenterol， 2011， 17(45)：5021-5027.
22. Flegal MC， Kuhlman SM. Anesthesia monitoring equipment for laboratory animals[J]. Lab Anim (NY)， 2004， 33(7)：31-36.
23. Lopez-Vidriero MT， Costello J， Clark TJ， et al. Effect of atropineon sputum production[J]. Thorax， 1975， 30(5)：543-547.
24. Burch JM， Denton JR， Noble RD. Physiologic rationale for abbre-viated laparotomy[J]. Surg Clin North Am， 1997， 77(4)：779-782.
25. Sessler DI. Mild perioperative hypothermia[J]. N Engl J Med， 1997， 336(24)：1730-1737.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Duodenal-Jejunal Bypass Animal Model in Goto-Kakizaki Rat

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