Expression of Gastrokine 1 in Gastric Cancer Tissues and Its Relationship with Clinicopathologic Characteristics_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Jiahui ^1,2 , XIAO Jiangwei ^2,3 , REN Mingyang ¹ , WEI Shoujiang ^2,3 , TIAN Xiaobing ⁴ , ZHOU Tong ^2,3 , XIE Xianyong ⁵ , WANG Chongshu ^2,3

1. The Second Department of General Surgery， Nanchong Central Hospital， The Second Clinical Institute of North Sichuan Medical College， Nanchong 637000， Sichuan Province， China;;
2. The First Department of General Surgery， Affiliated Hospital of North Sichuan Medical College， Nanchong 637000， Sichuan Proviote， China;;
3. Institute of Hepato-Biliary-Pancreas and Intestinal Disease， North Sichuan Medical College， Nanchong 637000， Sichuan Province， China;;

Corresponding author：

Keywords：

Gastrokine 1 gene; Gastric cancer; Helicobacter pylori

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Objective 　To clarify the role of gastrokine 1 in the process of formation and development of gastric cancer.
Methods 　The expressions of gastrokine 1 in gastric cancer and paracancerous tissues of 52 patients with gastric
cancer were detected by real-time fluorescence quantitative polymerase chain reaction （RT-PCR） and immunohistochemistry. Meanwhile the relationship of the expression level of gastrokine 1 with clinicopathologic characteristics were analyzed.
Results 　The expression levels of gastrokine 1 gene and protein in the gastric cancer tissues were significantly lower than those in the paracancerous tissues （P＜0.01）. No significant relationship was found between expression of gastrokine 1 gene and clinicopathologic features including tumor location， depth of invasion， differentiation， lymph node metastasis， tumor stage， gender， age， and preoperative peripheral blood CEA and CA19-9 levels （P＞0.05，respectively）. What’s more， the expression level of gastrokine 1 gene in gastric cancer tissues of Helicobacter pylori （HP）-positive patients was lower than that in the negative ones （P＜0.05）.
Conclusions 　Gastrokine 1 may play a significant role as an anti-oncogene in the process of the formation and development of gastric cancer. Its effect may become weak due to HP infection in gastric cancer patients.

Citation： CHEN Jiahui,XIAO Jiangwei,REN Mingyang,WEI Shoujiang,TIAN Xiaobing,ZHOU Tong,XIE Xianyong,WANG Chongshu. Expression of Gastrokine 1 in Gastric Cancer Tissues and Its Relationship with Clinicopathologic Characteristics. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(10): 1089-1093. doi: Copy

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20.	张永乐，薛英威，蓝秀文，等. 肿瘤标记物CA19-9、CEA对胃癌转移和预后预测价值的分析[J]. 哈尔滨医科大学学报， 2010， 44(2)：181-184， 188.
21.	Ucar E， Semerci E， Ustun H， et al. Prognostic value of preoperative CEA， CA19-9， CA72-4， and AFP levels in gastric cancer[J]. Adv Ther， 2008， 25(10)：1075-1084.
22.	Moss SF， Lee JW， Sabo E， et al. Decreased expression ofgastrokine 1 and the trefoil factor interacting protein TFIZ1/GKN2 in gastric cancer：influence of tumor histology and relationship to prognosis[J]. Clin Cancer Res， 2008， 14(13)：4161-4167.
23.	Yan GR， Xu SH， Tan ZL， et al. Proteomics characterization of gastrokine 1-induced growth inhibition of gastric cancer cells[J]. Proteomics， 2011， 11(18)：3657-3664.
24.	Yoon JH， Kang YH， Choi YJ， et al. Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial-mesenchymal transition in gastric cancers[J]. J Cancer Res Clin Oncol， 2011， 137(11)：1697-1704.
25.	Xing R， Li W， Cui J， et al. Gastrokine 1 induces senescence through p16/Rb pathway activation in gastric cancer cells[J]. Gut， 2012， 61(1)：43-52.

1. Jemal A， Bray F， Center MM， et al. Global cancer statistics[J]. CA Cancer J Clin， 2011， 61(2)：69-90.
2. Yang L. Incidence and mortality of gastric cancer in China[J]. World J Gastroenterol， 2006， 12(1)：17-20.
3. 梁寒. 胃癌转移规律及防治策略[J]. 中国普外基础与临床杂志， 2012， 19(1)：8-11.
4. Toback FG， Walsh-Reitz MM， Musch MW， et al. Peptide fragments of AMP-18， a novel secreted gastric antrum mucosal protein， are mitogenic and motogenic[J]. Am J Physiol Gastrointest Liver Physiol， 2003， 285(2)：G344-G353.
5. 杜建军，窦科峰，彭淑牖，等. 胃癌相关分泌蛋白CA11与胃癌关系的研究[J]. 中华胃肠外科杂志， 2003， 6(3)：187-190.
6. Shiozaki K， Nakamori S， Tsujie M， et al. Human stomach-specific gene， CA11， is down-regulated in gastric cancer[J]. Int J Oncol， 2001， 19(4)：701-707.
7. Rippa E， La Monica G， Allocca R， et al. Overexpression of gastrokine 1 in gastric cancer cells induces Fas-mediated apoptosis[J]. J Cell Physiol， 2011， 226(10)：2571-2578.
8. NCCN clinical practice guidelines in oncology gastric cancer (including cancer in the proximal 5 cm of the stomach)[EB/OL]. NCCN guidelines，http：//www. nccn. org/professionals/physician_gls/pdf/gastric. pdf.
9. 车晓波，刘文忠. 幽门螺杆菌常用诊断方法的评价[J]. 中华消化杂志， 2002， 22(11)：691-693.
10. Yoon JH， Song JH， Zhang C， et al. Inactivation of the gastrokine 1 gene in gastric adenomas and carcinomas[J]. J Pathol， 2011， 223(5)：618-625.
11. Oien KA， McGregor F， Butler S， et al. Gastrokine 1 is abundantly and specifically expressed in superficial gastric epithelium， down-regulated in gastric carcinoma， and shows high evolutionary conservation[J]. J Pathol， 2004， 203(3)：789-797.
12. 陈美娅，任建林，潘金水. AMP-18，一种新发现的胃黏膜保护因子[J]. 世界华人消化杂志， 2006， 14(8)：805-809.
13. Martin TE， Powell CT， Wang Z， et al. A novel mitogenicprotein that is highly expressed in cells of the gastric antrum mucosa[J]. Am J Physiol Gastrointest Liver Physiol， 2003， 285(2)：G332-G343.
14. Lacy ER， Morris GP， Cohen MM. Rapid repair of the surface epithelium in human gastric mucosa after acute superficial injury[J]. J Clin Gastroenterol， 1993， 17(Suppl 1)：S125-S135.
15. Nardone G， Martin G， Rocco A， et al. Molecular expression of Gastrokine 1 in normal mucosa and in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions[J]. Cancer Biol Ther， 2008， 7(12)：1890-1895.
16. Gisbert JP. Helicobacter pylori-related diseases：dyspepsia， ulcers and gastric cancer[J]. Gastroenterol Hepatol， 2011， 34(Suppl 2)：15-26.
17. 张启瑜. 钱礼腹部外科学[M]. 北京：人民卫生出版社， 2006：157-161.
18. Weck MN， Gao L， Brenner H. Helicobacter pylori infection and chronic atrophic gastritis：associations according to severity of disease[J]. Epidemiology， 2009， 20(4)：569-574.
19. Ohata H， Kitauchi S， Yoshimura N， et al. Progression of chronic atrophic gastritis associated with Helicobacter pylori infection increases risk of gastric cancer[J]. Int J Cancer， 2004， 109(1)：138-143.
20. 张永乐，薛英威，蓝秀文，等. 肿瘤标记物CA19-9、CEA对胃癌转移和预后预测价值的分析[J]. 哈尔滨医科大学学报， 2010， 44(2)：181-184， 188.
21. Ucar E， Semerci E， Ustun H， et al. Prognostic value of preoperative CEA， CA19-9， CA72-4， and AFP levels in gastric cancer[J]. Adv Ther， 2008， 25(10)：1075-1084.
22. Moss SF， Lee JW， Sabo E， et al. Decreased expression ofgastrokine 1 and the trefoil factor interacting protein TFIZ1/GKN2 in gastric cancer：influence of tumor histology and relationship to prognosis[J]. Clin Cancer Res， 2008， 14(13)：4161-4167.
23. Yan GR， Xu SH， Tan ZL， et al. Proteomics characterization of gastrokine 1-induced growth inhibition of gastric cancer cells[J]. Proteomics， 2011， 11(18)：3657-3664.
24. Yoon JH， Kang YH， Choi YJ， et al. Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial-mesenchymal transition in gastric cancers[J]. J Cancer Res Clin Oncol， 2011， 137(11)：1697-1704.
25. Xing R， Li W， Cui J， et al. Gastrokine 1 induces senescence through p16/Rb pathway activation in gastric cancer cells[J]. Gut， 2012， 61(1)：43-52.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Gastrokine 1 in Gastric Cancer Tissues and Its Relationship with Clinicopathologic Characteristics

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