Experimental Research of Renal Aquaporin-3 Expression in Obstructive Jaundice_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Dewei ¹ , GUAN Weiyu ¹ , LIU Jingang ² , WANG Yong. ²

1.Emergency Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110000, Liaoning Province, China;;
2.Shengjing Hospital of China Medical University, Shenyang 110000, Liaoning Province, China;

Corresponding author：

Keywords：

Obstructive jaundice; Acute renal failure; Aquaporin-3; Rat

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ObjectiveTo explore the value of Aquaporin-3 (AQP-3) on the detection of early renal function damage by investigating the expressions of renal AQP-3 mRNA and protein of rats with obstructive jaundice (OJ). MethodsForty mature male Wistar rats were divided into two groups randomly: experimental group (n=20) in which the model of OJ rats was established, and control group (n=20, sham operation group). The levels of serum total bilirubin (TBIL), direct bilirubin (DBIL), creatinine (Cr)， and blood urea nitrogen (BUN) were detected by fullautomatic biochemical analyzer on 7 d and 14 d after operation. The expressions of renal AQP-3 mRNA and protein of rats were detected by RT-PCR and Western blotting, respectively. ResultsThe levels of serum TBIL and DBIL were significantly higher on 14 d than those on 7 d after operation in experimental group (P=0.000), which were significantly higher than those at corresponding time point in control group (P=0.000), while the difference within control group was not significant (P=0.154). Thus, the OJ models of rats were established successfully. The difference of serum Cr levels of rats between inter-and intragroup were not significant (P gt;0.05). Serum BUN level on 14 d after operation in experimental group was significantly higher than those on 7 d after operation in experimental group and on 14 d after operation in control group (P=0.001), although serum Cr levels were not different between 7 d and 14 d after operation in control group (P=0.288). The expressions of AQP-3 protein of rats on 7 d and 14 d after operation in experimental group were significantly lower than those at corresponding time point in control group (P=0.033, P=0.000), meanwhile on 14 d after operation in experimental group was significantly lower than those on 7 d after operation in experimental group (P=0.000). The expressions of AQP-3 mRNA of rats on 7 d and 14 d after operation in experimental group were significantly higher than those at corresponding time point in control group (P=0.000), but the difference at different time point in two groups was not significant (P=0.139, P=0.059). ConclusionsThe changes of renal AQP-3 protein and mRNA expressions are prior to the changes of serum Cr and BUN levels of rats suffered from OJ complicated renal function damage, which are promised to improve the early diagnosis rate of renal function damage in rats with OJ.

Citation： ZHANG Dewei,GUAN Weiyu,LIU Jingang,WANG Yong.. Experimental Research of Renal Aquaporin-3 Expression in Obstructive Jaundice. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(11): 1175-1179. doi: Copy

1.	Fogarty BJ, Parks RW, Rowlands BJ, et al. Renal dysfunction in obstructive jaundice [J]. Br J Surg, 1995, 82(7): 877884.
2.	Ingoldby CJ, Mcpherson DA, Blumgart LH. Endotoxemia in human obstructive jaundice: effect of polymyxin B [J]. Am J Surg, 1984, 147(6): 766771.
3.	张丰深，何振平，段恒春．梗阻性黄疸并发肾功能损害的实验研究 [J]．第三军医大学学报， 2002， 24（9）： 10461047．.
4.	Agre P, Preston GM, Smith BL, et al. Aquaporin chip: the archetypal molecular water channel [J]. Am J Physiol, 1993, 265(4 Pt 2): 463476.
5.	Agre P, Nielsen S. The aquaporin family of water channels in kidney [J]. Néphrologie, 1996, 17(7): 409415.
6.	Nielsen S, Frkiaer J, Marples D, et al. Aquaporins in the kidney: from molecules to medicine [J]. Physiol Rev, 2002, 82(1): 205244.
7.	Ohshiro K, Yaoita E, Yoshida Y, et al. Expression and immunolocalization of AQP6 in intercalated cells of the rat kidney collecting duct [J]. Arch Histol Cytol, 2001, 64(3): 329338.
8.	刘金钢，王勇，宋军，等．生长激素对实验性梗阻性黄疸大鼠内毒素血症的防治 [J]．中华普通外科杂志， 2001， 16（8）： 493494．.
9.	Yüceyar S, Gümü瘙塂ta瘙塂 K, Ertürk S, et al. The role of oxygen free radicals in acute renal failure complicating obstructive jaundice: an experimental study [J]. HPB Surg, 1998, 10(6): 387393.
10.	Pereira JA, Torregrosa MA, MartínezRódenas F, et al. Increaced cardiac endocrine activity after common bile duct ligation in the rabbit. Atrial endocrine cells in obstructive jaundice [J]. Ann Surg, 1994, 219(1): 7378.
11.	Leung JC, Chan LY, Tsang AW, et al. Differential expression of aquaporins in the kidneys of streptozotocininduced diabetic mice [J]. Nephrology (Carlton), 2005, 10(1): 6372.
12.	Gong H, Wang W, Kwon TH, et al. Reduced renal expression of AQP2, pAQP2 and AQP3 in haemorrhagic shockinduced acute renal failure [J]. Nephrol Dial Transplant, 2003, 18(12): 25512559.
13.	Fernándezllama P, Andrews P, Turner R, et al. Decreased abundance of collecting duct aquaporins in postischemic renal failure in rats [J]. J Am Soc Nephrol, 1999, 10(8): 16581668.
14.	Combet S, Geffroy N, Berthonaud V, et al. Correction of agerelated polyuria by ddavp: molecular analysis of aquaporins and urea transporters [J]. Am J Physiol Renal Physiol, 2003, 284(1): F199F208.
15.	Kim SW, Gresz V, Rojek A, et al. Decreased expression of AQP2 and AQP4 water channels and Na, KATPase in kidney collecting duct in AQP3 null mice [J]. Biol Cell, 2005, 97(10): 765778.
16.	Dibas AI, Mia AJ, Yorio T. Aquaporins (water channels): role in vasopressinactivated water transport [J]. Proc Soc Exp Biol Med, 1998, 219(3): 183199.
17.	Fasshauer M, Klein J, Lossner U, et al. Suppression of aquaporin adipose gene expression by isoproterenol, TNF alpha, and dexamethasone [J]. Horm Metab Res, 2003, 35(4): 222227.
18.	Ge Y, Ahn D, Stricklett PK, et al. Collecting ductspecific knockout of endothelin1 alters vasopressin regulation of urine osmolality [J]. Am J Physiol Renal Physiol, 2005, 288(5): F912F920.
19.	Nejsum LN, Zelenina M, Aperia A, et al. Bidirectional regulation of AQP2 trafficking and recycling:involvement of AQP2S256 phosphorylation [J]. Am J Physiol Renal Physiol, 2005, 288(5): F930F938.
20.	Murer L, Addabbo F, Carmosino M, et al. Selective decrease in urinary aquaporin 2 and increase in prostaglandin E2 excretion is associated with postobstructive polyuria in human congenital hydronephrosis [J]. J Am Soc Nephrol, 2004, 15(10): 27052712.
21.	Devuyst O, Combet S, Cnops Y, et al. Regulation of no synthase isoforms in the peritoneum: implications for ultrafiltration failure in peritoneal dialysis [J]. Nephrol Dial Transplant, 2001, 16(3): 675678.
22.	Papakostas C, Bezirtzoglou E, Pitiakoudis M, et al. Endotoxinemia in the portal and the systemic circulation in obstructive jaundice [J]. Clin Exp Med, 2003, 3(2): 124128.
23.	谭毓铨，李有柱．梗阻性黄疸实验研究的进展与方向 [J]．中国实用外科杂志， 1996， 16（1）： 4951．.
24.	何宇，李昆，何振平．梗阻性黄疸肾缺血再灌注损害及其机制的研究 [J]．重庆医学， 2002， 31（12）： 11971200．.
25.	Deen PT, Verdijk MA, Knoers NV, et al. Requirement of human renal water channel aquaporin2 for vasopressindependent concentration of urine [J]. Science, 1994, 264(5155): 9295.

1. Fogarty BJ, Parks RW, Rowlands BJ, et al. Renal dysfunction in obstructive jaundice [J]. Br J Surg, 1995, 82(7): 877884.
2. Ingoldby CJ, Mcpherson DA, Blumgart LH. Endotoxemia in human obstructive jaundice: effect of polymyxin B [J]. Am J Surg, 1984, 147(6): 766771.
3. 张丰深，何振平，段恒春．梗阻性黄疸并发肾功能损害的实验研究 [J]．第三军医大学学报， 2002， 24（9）： 10461047．.
4. Agre P, Preston GM, Smith BL, et al. Aquaporin chip: the archetypal molecular water channel [J]. Am J Physiol, 1993, 265(4 Pt 2): 463476.
5. Agre P, Nielsen S. The aquaporin family of water channels in kidney [J]. Néphrologie, 1996, 17(7): 409415.
6. Nielsen S, Frkiaer J, Marples D, et al. Aquaporins in the kidney: from molecules to medicine [J]. Physiol Rev, 2002, 82(1): 205244.
7. Ohshiro K, Yaoita E, Yoshida Y, et al. Expression and immunolocalization of AQP6 in intercalated cells of the rat kidney collecting duct [J]. Arch Histol Cytol, 2001, 64(3): 329338.
8. 刘金钢，王勇，宋军，等．生长激素对实验性梗阻性黄疸大鼠内毒素血症的防治 [J]．中华普通外科杂志， 2001， 16（8）： 493494．.
9. Yüceyar S, Gümü瘙塂ta瘙塂 K, Ertürk S, et al. The role of oxygen free radicals in acute renal failure complicating obstructive jaundice: an experimental study [J]. HPB Surg, 1998, 10(6): 387393.
10. Pereira JA, Torregrosa MA, MartínezRódenas F, et al. Increaced cardiac endocrine activity after common bile duct ligation in the rabbit. Atrial endocrine cells in obstructive jaundice [J]. Ann Surg, 1994, 219(1): 7378.
11. Leung JC, Chan LY, Tsang AW, et al. Differential expression of aquaporins in the kidneys of streptozotocininduced diabetic mice [J]. Nephrology (Carlton), 2005, 10(1): 6372.
12. Gong H, Wang W, Kwon TH, et al. Reduced renal expression of AQP2, pAQP2 and AQP3 in haemorrhagic shockinduced acute renal failure [J]. Nephrol Dial Transplant, 2003, 18(12): 25512559.
13. Fernándezllama P, Andrews P, Turner R, et al. Decreased abundance of collecting duct aquaporins in postischemic renal failure in rats [J]. J Am Soc Nephrol, 1999, 10(8): 16581668.
14. Combet S, Geffroy N, Berthonaud V, et al. Correction of agerelated polyuria by ddavp: molecular analysis of aquaporins and urea transporters [J]. Am J Physiol Renal Physiol, 2003, 284(1): F199F208.
15. Kim SW, Gresz V, Rojek A, et al. Decreased expression of AQP2 and AQP4 water channels and Na, KATPase in kidney collecting duct in AQP3 null mice [J]. Biol Cell, 2005, 97(10): 765778.
16. Dibas AI, Mia AJ, Yorio T. Aquaporins (water channels): role in vasopressinactivated water transport [J]. Proc Soc Exp Biol Med, 1998, 219(3): 183199.
17. Fasshauer M, Klein J, Lossner U, et al. Suppression of aquaporin adipose gene expression by isoproterenol, TNF alpha, and dexamethasone [J]. Horm Metab Res, 2003, 35(4): 222227.
18. Ge Y, Ahn D, Stricklett PK, et al. Collecting ductspecific knockout of endothelin1 alters vasopressin regulation of urine osmolality [J]. Am J Physiol Renal Physiol, 2005, 288(5): F912F920.
19. Nejsum LN, Zelenina M, Aperia A, et al. Bidirectional regulation of AQP2 trafficking and recycling:involvement of AQP2S256 phosphorylation [J]. Am J Physiol Renal Physiol, 2005, 288(5): F930F938.
20. Murer L, Addabbo F, Carmosino M, et al. Selective decrease in urinary aquaporin 2 and increase in prostaglandin E2 excretion is associated with postobstructive polyuria in human congenital hydronephrosis [J]. J Am Soc Nephrol, 2004, 15(10): 27052712.
21. Devuyst O, Combet S, Cnops Y, et al. Regulation of no synthase isoforms in the peritoneum: implications for ultrafiltration failure in peritoneal dialysis [J]. Nephrol Dial Transplant, 2001, 16(3): 675678.
22. Papakostas C, Bezirtzoglou E, Pitiakoudis M, et al. Endotoxinemia in the portal and the systemic circulation in obstructive jaundice [J]. Clin Exp Med, 2003, 3(2): 124128.
23. 谭毓铨，李有柱．梗阻性黄疸实验研究的进展与方向 [J]．中国实用外科杂志， 1996， 16（1）： 4951．.
24. 何宇，李昆，何振平．梗阻性黄疸肾缺血再灌注损害及其机制的研究 [J]．重庆医学， 2002， 31（12）： 11971200．.
25. Deen PT, Verdijk MA, Knoers NV, et al. Requirement of human renal water channel aquaporin2 for vasopressindependent concentration of urine [J]. Science, 1994, 264(5155): 9295.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Experimental Research of Renal Aquaporin-3 Expression in Obstructive Jaundice

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