Expressions of Snail and VEGF Gene in Invasion Ductal Carcinoma of Breast_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XIONG Zhengwen , CAO Li , HU Haixia , LI Wei , ZHANG Huadong , XUN Fenghua

Department of Pathology， The 251th Hospital of PLA， Zhangjiakou 075000， Hebei Province， China;

Corresponding author：

Keywords：

Invasion ductal carcinoma of breast; Snail; Vascular endothelial growth factor; In situ hybridization

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ObjectiveTo investigate the expressions of Snail and VEGF gene in invasion ductal carcinoma tissues and analyze their clinicopathologic relationship. MethodsThe expressions of Snail and VEGF gene were detected on mammary gland hyperplasia (30 cases), intraductal breast cancer (30 cases), and invasion ductal carcinoma (70 cases) by in situ hybridization， to compare with the expression difference of the two genes in the different pathological changed tissues of mammary gland and among the clinicopathological facters of invasion ductal carcinoma as well as the relationship. ResultsThe expression rate of Snai mRNA in mammary gland hyperplasia， intraductal breast cancer, and invasion ductal carcinoma was 23.3% (7/30)， 46.7% (14/30), and 81.4% (57/70), respectively, there was statistical difference among them (χ 2=32.4, P lt;0.05)； The expression rate of VEGF mRNA in mammary gland hyperplasia， intraductal breast cancer, and invasion ductal carcinoma was 33.3% (10/30)， 50.0% (15/30), and 71.4% (50/70), respectively, there was statistical difference among them (χ 2=13.4, P lt;0.05). The expression rates of Snail mRNA and VEGF mRNA in lymphatic metatasis group were significantly higher than those in no lymphatic metatasis group 〔92.7% (38/41) vs. 65.5% (19/29)， χ 2=8.29, P lt;0.05； 85.4% (35/41) vs. 51.7% (15/29), χ 2=9.42, P lt;0.05， respectively 〕. The expression rates of Snail mRNA and VEGF mRNA in Ⅲ-Ⅳ stage of TNM clinical stage were significantly higher than those in Ⅰ-Ⅱ stage 〔939% (46/49) vs. 52.4% (11/21), χ 2=14.14， P lt;0.05； 81.6% (40/49) vs. 47.6% (10/21), χ 2=8.32， P lt;0.05〕. The expressions of Snail mRNA and VEGF mRNA were related to the expressions of ER, PR, HER-2, and vessel cancer embolus （P lt;0.01）. The expressions of Snail mRNA and VEGF mRNA were not related to age, tumor size, and histological grade (P gt;0.05). There was a positive correlation between the expressions of Snail mRNA and VEGF mRNA （r=0.67， P lt;0.05）. ConclusionsThe overexpressions of Snail mRNA and VEGF mRNA in invasion ductal carcinoma has a synergetic effect on occurrence and development, therefore, combined detecting the expressions of Snail mRNA and VEGF mRNA are some significance to predict infiltration and metastasis of the invasion ductal carcinoma.

Citation： XIONG Zhengwen,CAO Li,HU Haixia,LI Wei,ZHANG Huadong,XUN Fenghua,.. Expressions of Snail and VEGF Gene in Invasion Ductal Carcinoma of Breast. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(11): 1194-1199. doi: Copy

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2.	Kokudo T, Suzuki Y, Yoshimatsu Y, et al. Snail is required for TGFβ induced endothelialmesenchymal transition of embryonic stem cellderived endothelial cells [J]. J Cell Sci, 2008, 121(Pt20): 33173324.
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11.	Lee MY, Chou CY, Tang MJ, et al. Epithelialmesenchymal transition in cervical cancer: correlation with tumor progression, epidermal growth factor receptor overexpression, and snail upregulation [J]. Clin Cancer Res, 2008, 14(15): 47434750.
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16.	苏琼, 舒宽勇, 伍琼芳. PTEN、BCL2 和 CerbB2 蛋白在子宫内膜癌的表达及临床意义 [J]. 江西医药, 2011, 46(2): 114117.
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18.	熊正文, 范水平, 冯骥良，等. CD44与V EGF 在颅内转移瘤与胶质母细胞瘤中的表达研究 [J]. 实用医技杂志， 2005, 12(11): 32123215.
19.	李伟，熊正文，李宏伟. COX2、VEGF、Ecad在乳腺癌中的研究进展 [J]. 医学综述， 2010, 16(19): 29362940.
20.	Shimanuki Y, Takahashi K, Cui R, et al. Role of serum vascular endothelial growth factor in the prediction of angiogenesis and prognosis for nonsmall cell lung cancer [J] . Lung, 2005, 183(1): 2942.
21.	胡帅尔, 张雅洁, 崔玉梅, 等. VEGFA和VEGFC在乳腺癌组织中的表达及其意义 [J] . 癌症， 2005， 24(9): 10761079.
22.	Liang Y, Brekken RA, Hyder SM. Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the antiproliferative activity of antihormones [J]. Endocr Relat Cancer, 2006, 13(3): 905919.
23.	彭鹏，邹文蓉. p53, VEGF, Bcl2在进展期胃癌组织中的表达 [J]. 现代医药卫生, 2011, 27(2): 184185.
24.	付能高，陈小芬. VEGF和CD34在骨肉瘤组织中的表达及临床意义 [J]. 现代医药卫生, 2010, 26 (9): 12811282.
25.	张杰, 许俊龙, 张学东，等. 甲状腺乳头状癌VEGF、MMP9及COX2蛋白表达与淋巴道转移和血管生成的相关性 [J]. 肿瘤防治研究, 2010, 37(4): 441444.

1. Becker KF, Rosivatz E, Blechschmidt K, et al. Analysis of the Ecadherin repressor Snail in primary human cancers [J]. Cells Tissues Organs, 2007, 185(1): 204212.
2. Kokudo T, Suzuki Y, Yoshimatsu Y, et al. Snail is required for TGFβ induced endothelialmesenchymal transition of embryonic stem cellderived endothelial cells [J]. J Cell Sci, 2008, 121(Pt20): 33173324.
3. Mathias RA, Simpson RJ. Towards understanding epithelialmesenchymal transition: a proteomics perspective [J]. Biochim Biophys Acta, 2009, 1794(9): 13251331.
4. Voulgari A, Pintzas A. Epithelialmesenchymal transition in cancer metastasis: Mechanisms, markers and strategies to overcome drug resistance in the clinic [J]. Biochim Biophys Acta, 2009, 1796(2): 7590.
5. 寻风华，熊正文. 转录因子Snail与女性常见肿瘤关系研究进展 [J]. 中国妇幼保健杂志， 2011， 25（1）： 6972.
6. Dai J， Rabie AB. VEGF: an essential mediator of both angiogenesis and endochondral ossifycation [J]. J Dent Res， 2007， 86(10): 937950.
7. 程红, 戴林, 郭双评, 等译. 乳腺及女性生殖器官肿瘤病理学和遗传学 [M]. 北京: 人民卫生出版社, 2006: 13.
8. Sugimachi K， Tanaka S， Kameyama T， et al. Transcriptional repressor snail and progression of human hepatocellular carcinoma [J]. Clin Cancer Res, 2003, 9(7): 26572664.
9. 李伟，熊正文，李宏伟，等. COX2、VEGF、Ecad在乳腺癌组织中的表达及临床病理意义 [J].中国现代普通外科进展, 2010, 13(10): 766771.
10. Olmeda D, Montes A, MorenoBueno G, et al. Snai1 and snai2 collaborate on tumor growth and metastasis propertiesof mouse skin carcinoma cell lines [J]. Oncogene, 2008, 27(34): 46904701.
11. Lee MY, Chou CY, Tang MJ, et al. Epithelialmesenchymal transition in cervical cancer: correlation with tumor progression, epidermal growth factor receptor overexpression, and snail upregulation [J]. Clin Cancer Res, 2008, 14(15): 47434750.
12. Gholam D, Chebib A, Hauteville D, et al. Combined paclitaxel and cetuximab achieved a major response on the skin metastases of a patient with epidermal growth factor receptor positive, estrogen receptor negative, progesterone receptor negative and human epidermal growth factor receptor breast cancer [J]. Anticancer Drugs, 2007, 18(7): 835837.
13. Dunnwald LK， Rossing MA， Li Ｃi. Hormone receptor status, tumor characteristics, and prognosis: a prospective cohort of breast cancer patients [J]. Breast Cancer Res, 2007, 9(1): R6.
14. Rastelli F, Crispino S. Factors predictive of response to hormone therapy in breast cancer [J]. Tumori, 2008， 94(3): 370383.
15. 王宏波, 纪常生, 张军. p53和cerbB2与胃癌关系的研究进展 [J]. 中国普外基础与临床杂志， 2010， 17（8）： 880884.
16. 苏琼, 舒宽勇, 伍琼芳. PTEN、BCL2 和 CerbB2 蛋白在子宫内膜癌的表达及临床意义 [J]. 江西医药, 2011, 46(2): 114117.
17. 刘萍，田薇薇，梁冰. 非小细胞肺癌组织中CerbB2蛋白的表达 [J]. 郑州大学学报(医学版), 2006, 41(5): 917918.
18. 熊正文, 范水平, 冯骥良，等. CD44与V EGF 在颅内转移瘤与胶质母细胞瘤中的表达研究 [J]. 实用医技杂志， 2005, 12(11): 32123215.
19. 李伟，熊正文，李宏伟. COX2、VEGF、Ecad在乳腺癌中的研究进展 [J]. 医学综述， 2010, 16(19): 29362940.
20. Shimanuki Y, Takahashi K, Cui R, et al. Role of serum vascular endothelial growth factor in the prediction of angiogenesis and prognosis for nonsmall cell lung cancer [J] . Lung, 2005, 183(1): 2942.
21. 胡帅尔, 张雅洁, 崔玉梅, 等. VEGFA和VEGFC在乳腺癌组织中的表达及其意义 [J] . 癌症， 2005， 24(9): 10761079.
22. Liang Y, Brekken RA, Hyder SM. Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the antiproliferative activity of antihormones [J]. Endocr Relat Cancer, 2006, 13(3): 905919.
23. 彭鹏，邹文蓉. p53, VEGF, Bcl2在进展期胃癌组织中的表达 [J]. 现代医药卫生, 2011, 27(2): 184185.
24. 付能高，陈小芬. VEGF和CD34在骨肉瘤组织中的表达及临床意义 [J]. 现代医药卫生, 2010, 26 (9): 12811282.
25. 张杰, 许俊龙, 张学东，等. 甲状腺乳头状癌VEGF、MMP9及COX2蛋白表达与淋巴道转移和血管生成的相关性 [J]. 肿瘤防治研究, 2010, 37(4): 441444.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expressions of Snail and VEGF Gene in Invasion Ductal Carcinoma of Breast

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