Expression and Significance of Motilin in Gastric Cancer Tissues_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Hongbo ^1,2 , XU Luo ¹

1.Staff Room of Pathophysiology, Medical College of Qingdao University, Qingdao 266021, Shangdong Province, China;;
2.Department of Gastrointestinal Surgery, The People’s Hospital of Jimo City, Jimo 266200, Shangdong Province, China;

Corresponding author：

Keywords：

Gastric cancer; Paracancerous tissues; Motilin; Expression; Immunohistochemistry

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ObjectiveTo detect the expression of motilin in gastric cancer tissues and to explore the relationship between motilin protein expression and clinicopathologic characteristics of gastric cancer. MethodsThe immunohistochemical staining was used to detect the expression of motilin protein in gastric cancer, paracancerous tissues, and normal gastric mucosa tissues. The relationship between motilin protein expression and clinicopathologic characteristics of gastric cancer was analyzed. ResultsThe expression of motilin protein in gastric cancer tissues (1 206.43±631.67) was significantly higher than that in normal gastric mucosa tissues and paracancerous tissues, respectively (P lt;0.01). The difference of motilin protein expression between normal gastric mucosa tissues and paracancerous tissues was not significant (P gt;0.05). The expression of motilin protein in gastric cancer was correlated with the site of tumor, differentiation degree, and lymph node metastasis (P lt;0.05). ConclusionMotilin may participate in the carcinogenesis of gastric cancer, and correlated with the invasion and metastasis of gastric cancer.

Citation： WANG Hongbo ,XU Luo. Expression and Significance of Motilin in Gastric Cancer Tissues. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(2): 183-186. doi: Copy

1.	Cunningham SC, Kamangar F, Kim MP, et al. Claudin4, mitogenactivated protein kinase kinase 4, and stratifin are markers of gastric adenocarcinoma precursor lesions [J]. Cancer Epidemiol Biomarkers Prev, 2006, 15(2): 281287.
2.	Konturek PC, Kania J, Konturek JW, et al. H. pylori infection, atrophic gastritis, cytokines, gastrin, COX2, PPAR γ and impaired apoptosis in gastric carcinogenesis [J]. Med Sci Monit, 2003, 9(7): SR53SR66.
3.	Hu C, Yi C, Hao Z, et al. The effect of somatostatin and SSTR3 on proliferation and apoptosis of gastric cancer cells [J]. Cancer Biol Ther, 2004, 3(8): 726730.
4.	Matsubara J, Yamada Y, Nakajima TE, et al. Clinical significance of insulinlike growth factor type 1 receptor and epidermal growth factor receptor in patients with advanced gastric cancer [J]. Oncology, 2008, 74(12): 7683.
5.	Ito M, Tanaka S, Maeda M, et al. Role of the gastringastrin receptor system in the expansive growth of human gastric neoplasms [J]. Digestion, 2008, 78(23): 163170.
6.	叶序卷, 王立. 胃癌与相关胃肠激素的关系研究进展 [J]. 国际消化病杂志, 2006, 26(4): 253256.
7.	Maev IV, Mel’nikova EV, Kashin SV, et al. Serologic screening for preneoplastic pathology and early stomach cancer [J]. Klin Med (Mosk), 2008, 86(11): 4348.
8.	Kamerling IM, Van Haarst AD, Burggraaf J, et al. Motilin effects on the proximal stomach in patients with functional dyspepsia and healthy volunteers [J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(5): G776G781.
9.	Sanger GJ. Motilin, ghrelin and related neuropeptides as targets for the treatment of GI diseases [J]. Drug Discov Today, 2008, 13(56): 234239.
10.	Pendleton H, Ekman R, Olsson R, et al. Motilin concentrations in relation to gastro intestinal dysmotility in diabetes mellitus [J]. Eur J Intern Med, 2009, 20(6): 654659.
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12.	Itoh Z. Motilin and clinical application [J]. Peptides, 1997, 18(4): 593608.
13.	Xu L, Depoortere I, Tang M, et al. Identification and expression of the motilin precursor in the guinea pig [J]. FEBS Lett, 2001, 490(12): 710.
14.	Xu L, Depoortere I, Tomasetto C, et al. Evidence for the presence of motilin, ghrelin, and the motilin and ghrelin receptor in neurons of the myenteric plexus [J]. Regul Pept, 2005, 124(13): 119125.
15.	Takeshita E, Matsuura B, Dong M, et al. Molecular characterization and distribution of motilin family receptors in the human gastrointestinal tract [J]. J Gastroenterol, 2006, 41(3): 223230.
16.	Ter Beek WP, Muller ES, van den Berg M, et al. Motilin receptor expression in smooth muscle, myenteric plexus, and mucosa of human inflamed and noninflamed intestine [J]. Inflamm Bowel Dis, 2008, 14(5): 612619.
17.	Ozaki K, Yogo K, Sudo H, et al. Effects of mitemcinal (GM611), an acidresistant nonpeptide motilin receptor agonist on the gastrointestinal contractile activity in conscious dogs [J]. Pharmacology, 2007, 79(4): 223235.
18.	McCallum RW, Cynshi O. Clinical trial: effect of mitemcinal (a motilin agonist) on gastric emptying in patients with gastroparesisa randomized, multicentre, placebocontrolled study [J]. Aliment Pharmacol Ther, 2007, 26(8): 11211130.
19.	De Smet B, Mitselos A, Depoortere I. Motilin and ghrelin as prokinetic drug targets [J]. Pharmacol Ther, 2009, 123(2): 207223.
20.	Trudel L, Tomasetto C, Rio MC, et al. Ghrelin/motilinrelated peptide is a potent prokinetic to reverse gastric postoperative ileus in rat [J]. Am J Physiol Gastrointest Liver Physiol, 2002, 282(6): G948G952.
21.	邓钢明, 袁庆丰. 胃癌患者血浆胃动素与胃收缩运动的关系探讨 [J]. 蚌埠医学院学报, 2001, 26(3): 245.
22.	龙宪和. 胃肠肝等癌症患者血浆胃动素观察的临床意义 [J]. 标记免疫分析与临床, 2003, 10(3): 181182.
23.	吕宾, 叶再元, 朱林喜, 等．胃癌组织及癌区动、静脉血胃肠激素的研究 [J]. 世界华人消化杂志, 2000, 8(12): 14291430.
24.	杨小慧, 刘勇刚, 陈海鹰, 等. 消化道肿瘤患者化疗前后血浆胃动素和降钙素基因相关肽的变化 [J]. 中国基层医药, 2008, 15(1): 2021.
25.	刘建平. 化浊解毒汤对慢性萎缩性胃炎大鼠MTL、SS、ET的影响 [D]. 河北: 河北医科大学, 2009: 2639.

1. Cunningham SC, Kamangar F, Kim MP, et al. Claudin4, mitogenactivated protein kinase kinase 4, and stratifin are markers of gastric adenocarcinoma precursor lesions [J]. Cancer Epidemiol Biomarkers Prev, 2006, 15(2): 281287.
2. Konturek PC, Kania J, Konturek JW, et al. H. pylori infection, atrophic gastritis, cytokines, gastrin, COX2, PPAR γ and impaired apoptosis in gastric carcinogenesis [J]. Med Sci Monit, 2003, 9(7): SR53SR66.
3. Hu C, Yi C, Hao Z, et al. The effect of somatostatin and SSTR3 on proliferation and apoptosis of gastric cancer cells [J]. Cancer Biol Ther, 2004, 3(8): 726730.
4. Matsubara J, Yamada Y, Nakajima TE, et al. Clinical significance of insulinlike growth factor type 1 receptor and epidermal growth factor receptor in patients with advanced gastric cancer [J]. Oncology, 2008, 74(12): 7683.
5. Ito M, Tanaka S, Maeda M, et al. Role of the gastringastrin receptor system in the expansive growth of human gastric neoplasms [J]. Digestion, 2008, 78(23): 163170.
6. 叶序卷, 王立. 胃癌与相关胃肠激素的关系研究进展 [J]. 国际消化病杂志, 2006, 26(4): 253256.
7. Maev IV, Mel’nikova EV, Kashin SV, et al. Serologic screening for preneoplastic pathology and early stomach cancer [J]. Klin Med (Mosk), 2008, 86(11): 4348.
8. Kamerling IM, Van Haarst AD, Burggraaf J, et al. Motilin effects on the proximal stomach in patients with functional dyspepsia and healthy volunteers [J]. Am J Physiol Gastrointest Liver Physiol, 2003, 284(5): G776G781.
9. Sanger GJ. Motilin, ghrelin and related neuropeptides as targets for the treatment of GI diseases [J]. Drug Discov Today, 2008, 13(56): 234239.
10. Pendleton H, Ekman R, Olsson R, et al. Motilin concentrations in relation to gastro intestinal dysmotility in diabetes mellitus [J]. Eur J Intern Med, 2009, 20(6): 654659.
11. National Comprehensive Cancer Network. NCCN胃癌临床实践指南（中国版）专家组. 胃癌临床实践指南(中国版) [S]. 2009： 20.
12. Itoh Z. Motilin and clinical application [J]. Peptides, 1997, 18(4): 593608.
13. Xu L, Depoortere I, Tang M, et al. Identification and expression of the motilin precursor in the guinea pig [J]. FEBS Lett, 2001, 490(12): 710.
14. Xu L, Depoortere I, Tomasetto C, et al. Evidence for the presence of motilin, ghrelin, and the motilin and ghrelin receptor in neurons of the myenteric plexus [J]. Regul Pept, 2005, 124(13): 119125.
15. Takeshita E, Matsuura B, Dong M, et al. Molecular characterization and distribution of motilin family receptors in the human gastrointestinal tract [J]. J Gastroenterol, 2006, 41(3): 223230.
16. Ter Beek WP, Muller ES, van den Berg M, et al. Motilin receptor expression in smooth muscle, myenteric plexus, and mucosa of human inflamed and noninflamed intestine [J]. Inflamm Bowel Dis, 2008, 14(5): 612619.
17. Ozaki K, Yogo K, Sudo H, et al. Effects of mitemcinal (GM611), an acidresistant nonpeptide motilin receptor agonist on the gastrointestinal contractile activity in conscious dogs [J]. Pharmacology, 2007, 79(4): 223235.
18. McCallum RW, Cynshi O. Clinical trial: effect of mitemcinal (a motilin agonist) on gastric emptying in patients with gastroparesisa randomized, multicentre, placebocontrolled study [J]. Aliment Pharmacol Ther, 2007, 26(8): 11211130.
19. De Smet B, Mitselos A, Depoortere I. Motilin and ghrelin as prokinetic drug targets [J]. Pharmacol Ther, 2009, 123(2): 207223.
20. Trudel L, Tomasetto C, Rio MC, et al. Ghrelin/motilinrelated peptide is a potent prokinetic to reverse gastric postoperative ileus in rat [J]. Am J Physiol Gastrointest Liver Physiol, 2002, 282(6): G948G952.
21. 邓钢明, 袁庆丰. 胃癌患者血浆胃动素与胃收缩运动的关系探讨 [J]. 蚌埠医学院学报, 2001, 26(3): 245.
22. 龙宪和. 胃肠肝等癌症患者血浆胃动素观察的临床意义 [J]. 标记免疫分析与临床, 2003, 10(3): 181182.
23. 吕宾, 叶再元, 朱林喜, 等．胃癌组织及癌区动、静脉血胃肠激素的研究 [J]. 世界华人消化杂志, 2000, 8(12): 14291430.
24. 杨小慧, 刘勇刚, 陈海鹰, 等. 消化道肿瘤患者化疗前后血浆胃动素和降钙素基因相关肽的变化 [J]. 中国基层医药, 2008, 15(1): 2021.
25. 刘建平. 化浊解毒汤对慢性萎缩性胃炎大鼠MTL、SS、ET的影响 [D]. 河北: 河北医科大学, 2009: 2639.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression and Significance of Motilin in Gastric Cancer Tissues

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