Expression of Zonula Occludens-1 and Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CAO Hongqing , HUANG Heguang , CHEN Yanchang , LI Hongsen.

Department of General Surgery, Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China;

Corresponding author：

Keywords：

Severe acute pancreatitis; Rat; Zonula occludens-1; Intestinal mucosal barrier

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ObjectiveTo study the expression of zonula occluden-1 (ZO-1) in ileum tissues and the possible mechanism of intestinal mucosal barrier injury in rats with severe acute pancreatitis (SAP). MethodsFifty SD male rats were randomly divided into sham operation group and SAP group, then SAP group was divided into four subgroups with 10 rats in each subgroup according to the sampling time of 3, 6, 12, and 24 h. The SAP model was made by injecting 5% bovine sodium deoxycholate into biliarypancreatic duct with Aho’s method. The rats were killed at 3, 6, 12, and 24 h after making model. The rats in the sham operation group were killed directly. Tumor necrosis factor-α (TNF-α), diamine oxidase (DAO), and histological changes in pancreatic and intestinal pathologies were observed. At the same time, the ZO-1 protein and mRNA expressions of ileum tissues were detected by immunohistochemistry and RT-PCR, respectively. ResultsCompared with the sham operation group 〔TNF-α: (10.83±0.96) ng/L; DAO: (354.79±3.67) U/L; ZO-1 protein: (10.40±0.45) score; ZO-1 mRNA: 0.878±0.014 8〕, the levels of TNF-α at different time 〔3 h: (125.30±0.94) ng/L; 6 h: (181.89±4.93) ng/L; 12 h: (230.58±1.28) ng/L; 24 h: (198.89±4.83) ng/L〕 were significantly higher (P lt;0.05), the activities of DAO 〔3 h: (235.77±0.67) U/L; 6 h: (117.22±5.58) U/L; 12 h: (106.69±1.39) U/L; 24 h: (91.18±1.09) U/L〕 were significantly lower (P lt;0.05), ZO-1 protein 〔3 h: (8.70±0.22) score; 6 h: (3.73±0.19) score; 12 h: (3.92±0.22) score; 24 h: (4.29±0.30) score〕 and mRNA (3 h: 0.806±0.020 7; 6 h: 0.370±0.015 8; 12 h: 0.502±0.019 2; 24 h: 0.562±0.030 3) expressions of the ileum tissues were significantly lower (P lt;0.05) in the SAP group; Meanwhile, the necrosis of ileum mucous membrane chorioepithelium, angiorrhexis and hemorrhage, and inflammatory cell infiltration in the pancreatic and ileum tissues were also observed. ConclusionThe decrease of expression of ZO-1 in ileum tissues is one of the vital causes for mucosal barrier injury in SAP, probably through acts the excessive release of inflammatory cytokines TNF-α and the decrease of DAO activity.

Citation： CAO Hongqing,HUANG Heguang,CHEN Yanchang,LI Hongsen.. Expression of Zonula Occludens-1 and Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(3): 240-244. doi: Copy

1.	Ammori BJ. Gut barrier dysfunction in patients with acute pancreatitis [J]. J Hepatobiliary Pancreat Surg, 2002, 9(4): 411412．.
2.	杨雪菲, 王春友．对重型急性胰腺炎的认识现况 [J]．中国综合临床， 2005, 21(1): 9596．.
3.	Furuya T, Soeno T, Komatsu M． Strategy for bacterial translocation in acute pancreatitis [J]．Nippon Shokakibyo Gakkai Zasshi， 2004, 101(5)：502509．.
4.	Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis [J]. Scand J Gastroenterol, 1980, 15(4): 411416．.
5.	Soslow RA, Dannenberg AJ, Rush D, et al. COX2 is expressed in human pulmonary, colonic, and mammary tumors [J]. Cancer, 2000, 89(12): 26372645.
6.	Nusrat A, Parkos CA, Verkade P, et al. Tight junctions are membrane microdomains [J]. J Cell Sci, 2000, 113 (Pt 10): 17711781．.
7.	Khler K， Zahraoui A. Tight junction: a coordinator of cell signalling and membrane trafficking [J]. Biol Cell, 2005, 97(8): 659665.
8.	Stevenson BR, Siliciano JD, Mooseker MS, et al. Identification of ZO1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia [J]. J Cell Biol, 1986, 103(3): 755766．.
9.	Sawada N, Murata M, Kikuchi K, et al. Tight junctions and human diseases [J]. Med Electron Microsc, 2003, 36(3): 147156．.
10.	高志光, 秦环龙. 肠上皮细胞紧密连接的生物学功能及在肠屏障中的作用 [J]. 肠外与肠内营养， 2005, 12(5): 299302．.
11.	鲍欢, 包仕尧. 缺氧后血脑屏障紧密连接变化的分子机制 [J]. 国外医学血管疾病分册, 2005, 13(8): 604608.
12.	Umeda K, Matsui T, Nakayama M, et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO1 [J]. J Biol Chem, 2004, 279(43): 4478544794．.
13.	Youakim A, Ahdieh M. Interferongamma decreases barrier function in T84 cells by reducing ZO1 levels and disrupting apical actin [J]. Am J Physiol, 1999, 276(5 Pt 1): G1279G1288.
14.	Weiler F, Marbe T, Scheppach W, et al. Influence of protein kinase C on transcription of the tight junction elements ZO1 and occludin [J]. J Cell Physiol, 2005, 204(1): 8386.
15.	Cario E, Gerken G, Podolsky DK. Tolllike receptor 2 enhances ZO1associated intestinal epithelial barrier integrity via protein kinase C [J]. Gastroenterology, 2004, 127(1): 224238.
16.	杨植, 杨志英, 唐伟松, 等. 肿瘤坏死因子在猴急性胰腺炎中的作用及生长抑素的治疗效果 [J]．普外基础与临床杂志，1996, 3(1): 46．.
17.	王昌成, 马兴刚, 徐淮, 等. 血清IL6、IL8和TNFα在早期诊断重症急性胰腺炎中的价值 [J]. 中华急诊医学杂志, 2001, 10(4): 252253.
18.	Blikslager AT, Moeser AJ, Gookin JL, et al. Restoration of barrier function in injured intestinal mucosa [J]. Physiol Rev, 2007, 87(2): 545564．.
19.	Li Q, Zhang Q, Wang M, et al. Interferongamma and tumor necrosis factoralpha disrupt epithelial barrier function by altering lipid composition in membrane microdomains of tight junction [J]. Clin Immunol, 2008, 126(1): 6780．.
20.	黎君友, 吕艺, 付小兵, 等. 二胺氧化酶在创伤后肠道损伤中变化及意义 [J]．中国危重病急救医学, 2000, 12(8): 482484．.

1. Ammori BJ. Gut barrier dysfunction in patients with acute pancreatitis [J]. J Hepatobiliary Pancreat Surg, 2002, 9(4): 411412．.
2. 杨雪菲, 王春友．对重型急性胰腺炎的认识现况 [J]．中国综合临床， 2005, 21(1): 9596．.
3. Furuya T, Soeno T, Komatsu M． Strategy for bacterial translocation in acute pancreatitis [J]．Nippon Shokakibyo Gakkai Zasshi， 2004, 101(5)：502509．.
4. Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis [J]. Scand J Gastroenterol, 1980, 15(4): 411416．.
5. Soslow RA, Dannenberg AJ, Rush D, et al. COX2 is expressed in human pulmonary, colonic, and mammary tumors [J]. Cancer, 2000, 89(12): 26372645.
6. Nusrat A, Parkos CA, Verkade P, et al. Tight junctions are membrane microdomains [J]. J Cell Sci, 2000, 113 (Pt 10): 17711781．.
7. Khler K， Zahraoui A. Tight junction: a coordinator of cell signalling and membrane trafficking [J]. Biol Cell, 2005, 97(8): 659665.
8. Stevenson BR, Siliciano JD, Mooseker MS, et al. Identification of ZO1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia [J]. J Cell Biol, 1986, 103(3): 755766．.
9. Sawada N, Murata M, Kikuchi K, et al. Tight junctions and human diseases [J]. Med Electron Microsc, 2003, 36(3): 147156．.
10. 高志光, 秦环龙. 肠上皮细胞紧密连接的生物学功能及在肠屏障中的作用 [J]. 肠外与肠内营养， 2005, 12(5): 299302．.
11. 鲍欢, 包仕尧. 缺氧后血脑屏障紧密连接变化的分子机制 [J]. 国外医学血管疾病分册, 2005, 13(8): 604608.
12. Umeda K, Matsui T, Nakayama M, et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO1 [J]. J Biol Chem, 2004, 279(43): 4478544794．.
13. Youakim A, Ahdieh M. Interferongamma decreases barrier function in T84 cells by reducing ZO1 levels and disrupting apical actin [J]. Am J Physiol, 1999, 276(5 Pt 1): G1279G1288.
14. Weiler F, Marbe T, Scheppach W, et al. Influence of protein kinase C on transcription of the tight junction elements ZO1 and occludin [J]. J Cell Physiol, 2005, 204(1): 8386.
15. Cario E, Gerken G, Podolsky DK. Tolllike receptor 2 enhances ZO1associated intestinal epithelial barrier integrity via protein kinase C [J]. Gastroenterology, 2004, 127(1): 224238.
16. 杨植, 杨志英, 唐伟松, 等. 肿瘤坏死因子在猴急性胰腺炎中的作用及生长抑素的治疗效果 [J]．普外基础与临床杂志，1996, 3(1): 46．.
17. 王昌成, 马兴刚, 徐淮, 等. 血清IL6、IL8和TNFα在早期诊断重症急性胰腺炎中的价值 [J]. 中华急诊医学杂志, 2001, 10(4): 252253.
18. Blikslager AT, Moeser AJ, Gookin JL, et al. Restoration of barrier function in injured intestinal mucosa [J]. Physiol Rev, 2007, 87(2): 545564．.
19. Li Q, Zhang Q, Wang M, et al. Interferongamma and tumor necrosis factoralpha disrupt epithelial barrier function by altering lipid composition in membrane microdomains of tight junction [J]. Clin Immunol, 2008, 126(1): 6780．.
20. 黎君友, 吕艺, 付小兵, 等. 二胺氧化酶在创伤后肠道损伤中变化及意义 [J]．中国危重病急救医学, 2000, 12(8): 482484．.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Zonula Occludens-1 and Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis

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