Effects of Cucurmosin on Apoptosis in Human Pancreatic Cancer Cell Line SW1990_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YIN Qiang ¹ , HUANG Heguang ¹ , XIE Jieming ² , CHEN Minghuang ³ , ZHANG Baoming ¹ , WANG Congfei ¹

1.Department of General Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China;;
2.Department of Pharmacology, Fujian Medical University, Fuzhou 350004, Fujian Province, China;;
3.State Structural Chemistry Key Laboratory of Fujian Institute of Research on Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, Fujian Province, China;

Corresponding author：

Keywords：

Pancreatic neoplasm; Cucurmosin protein; Apoptosis

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ObjectiveTo investigate the possible mechanism of cucurmosin on apoptosis in human pancreatic cancer cell line SW1990 in vitro. MethodsThe inhibition of cucurmosin on SW1990 cell was detected by MTT assay, the apoptosis was observed by transmission electron microscope, the apoptosis rate was analyzed by flow cytometry, and the protein level of caspase3 was determined by Western blot. ResultsAfter exposure to cucurmosin at 1.25, 2.50, 5.00, 10.00, 20.00, 40.00, and 80.00 μg/ml for 24, 48, and 72 h, the proliferation of SW1990 cell was inhibited in a time-and dose-dependent manner (P lt;0.05). At 72 h after 40.00 μg/ml cucurmosin treatment, the typical apoptosis changes and apoptotic bodies were observed by transmission electron microscope. After exposure to cucurmosin at 0, 2.50, 10.00, and 40.00 μg/ml for 72 h, the apoptosis rate increased gradually as (0.30±0.11)%, (18.93±1.06)%, (28.00±2.07)%, and (49.93±3.25)%, respectively (P lt;0.05). The expression of caspase-3 protein was elevated gradually (P lt;0.05). ConclusionCucurmosin may induce the apoptosis of pancreatic cancer cell through up-regulating the expression of caspase-3.

Citation： YIN Qiang ,HUANG Heguang,XIE Jieming,CHEN Minghuang,ZHANG Baoming,WANG Congfei. Effects of Cucurmosin on Apoptosis in Human Pancreatic Cancer Cell Line SW1990. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(4): 380-383. doi: Copy

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1. Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2007 [J]. CA Cancer J Clin, 2007, 57(1): 4366.
2. 孙备, 陈华. 胰腺癌外科治疗的现状与展望 [J]. 中国普外基础与临床杂志, 2008, 15(8): 547549.
3. Müller MW, Friess H, Kninger J. Factors influencing survival after bypass procedures in patients with advanced pancreatic adenocarcinomas [J]. Am J Surg, 2008, 195(2): 221228.
4. Chen MH, Ye XM, Cai JH, et al. Crystallization and preliminary crystallographic study of cucurmosin, a ribosomeinactivating protein from the sarcocarp of Cucurbita moschata [J]. Acta Crystallogr D Biol Crystallogr, 2000, 56(Pt 5): 665666.
5. Endo Y, Mitsui K, Motizuki M, et al. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins [J]. J Biol Chem, 1987, 262(12): 59085912.
6. Peumans WJ, Hao Q, Van Damme EJ. Ribosomeinactivating proteins from plants: more than RNA Nglycosidases? [J]. FASEB J, 2001, 15(9): 14931506.
7. Stirpe F, Battelli MG. Ribosomeinactivating proteins: progress and problems. [J]. Cell Mol Life Sci, 2006, 63(16): 18501866.
8. 谢捷明, 陈明晃, 许建华, 等. 南瓜蛋白在制药中的应用 [P].中国, 发明专利, 200510119741.2, 20051103.
9. 孙建成, 谢捷明, 张昆仲, 等. 南瓜蛋白与STI571体外联合抗K562细胞的作用 [J]. 福建医科大学学报, 2008, 42(1): 3032, 41.
10. 宋其蔓, 孙洞箫. 细胞凋亡与p21关系的研究进展 [J]. 医学综述, 2008, 14(6): 816818.
11. 张伟辉, 郑彪, 薛东波, 等. 青蒿素诱导急性胰腺炎胰腺腺泡细胞凋亡的实验研究 [J]. 中国普外基础与临床杂志, 2007, 14(4): 388391.
12. 张晓田, 宋天保. Caspase3与细胞凋亡的研究 [J]. 医学综述， 2002, 8(11): 621623.
13. Zhang Y, Goodyer C, LeBlanc A. Selective and protracted apoptosis in human primary neurons microinjected with active caspase3, 6, 7, and 8 [J]. J Neurosci, 2000, 20(22): 83848389.
14. Cryns V, Yuan J. Protease to die for [J]. Genes Dev, 1998, 12(11): 15511570.
15. 龙辉, 吴清明, 马松林, 等. Midkine和Caspase3在胰腺癌中的表达与细胞凋亡的关系 [J]. 世界华人消化杂志, 2008, 16(35): 40154019.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Cucurmosin on Apoptosis in Human Pancreatic Cancer Cell Line SW1990

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