Establishing Rat Model of Incomplete Adhesive Intestinal Obstruction by Serosal Stripping Method_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Donghong ¹ , LI Liang ² , SHENG Li ³ , LIU Yuanjie ⁴ , HUANG Xiaoling ⁵ , WANG Zili ⁶ , TANG Xiaoyong ⁷

1.Department of Medical Administration, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu Province, China;;
2.Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China;;
3.Department of Ultrasound Division, Gansu Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu Province, China;;
4.Department of Clinical Laboratory, Gansu Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu Province, China;;

Corresponding author：

Keywords：

Serosal stripping method; Incomplete adhesive intestinal obstruction; Model; Rat

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ObjectiveTo study a new method for establishing the rat model of incomplete adhesive intestinal obstruction. MethodsThe serosal stripping method was adopted, the general health condition changes of rats were observed on day 3, 5, and 7 after modeling, meanwhile the weight was measured, the superior mesenteric artery flow velocity and small intestine wall thickness were observed via the color Doppler ultrasound and orthostatic X ray. The level of D-lactate acid in the blood plasma, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the intestine tissue were tested. The pathological changes of the intestine tissue were observed. ResultsCompared with the normal group (no treatment was done), the general health conditions of rats were worse, the weight significantly decreased (P lt;0.01), the superior mesenteric artery blood flow velocity significantly increased and small intestinal wall was thickened (P lt;0.05, P lt;0.01), airfluid level or a great deal of seroperitoneum and pneumatosis flat appeared via orthostatic abdominal X-ray, the level of D-lactic acid in the blood plasma and the content of MDA in the intestine tissue significantly increased on day 5 after modeling (P lt;0.05, P lt;0.01), the activity of SOD in the intestine tissue significantly decreased on day 7 after modeling in the model group (P lt;0.05). The pathological changes consistented with adhesive intestinal obstruction via pathological observation. On the 7th day, the rat model of incomplete adhesive intestinal obstruction was successfully built . ConclusionThe rat model of incomplete adhesive intestinal obstruction is completed by serosal stripping method.

Citation： WANG Donghong ,LI Liang,SHENG Li,LIU Yuanjie,HUANG Xiaoling,WANG Zili,TANG Xiaoyong. Establishing Rat Model of Incomplete Adhesive Intestinal Obstruction by Serosal Stripping Method. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(4): 384-390. doi: Copy

1.	罗东林, 张胜本, 刘宝华, 等. 老年急性肠梗阻的临床特点及其治疗(附150例报告) [J]. 中国普外基础与临床杂志, 2002, 9(6): 436437.
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18.	杨牧祥, 于文涛, 徐华洲, 等. 中风康对局灶性脑缺血大鼠脑组织SOD MDA的影响 [J]. 中医药学刊, 2005, 23(8): 13651367.
19.	李宗辉. 藏雪莲水提取物对小白鼠组织内MDA含量的影响 [J]. 安徽农业科学, 2008, 36(35): 15510, 15534.
20.	Smith SM, Eng RH, Buccini F. Use of Dlactic acid measurements in the diagnosis of bacterial infections [J]. J Infect Dis, 1986, 154(4): 658664.
21.	卢良声, 庄永敬, 吴桂荣, 等. 基于CT数据肠系膜上动脉三维重建及解剖学研究 [J]. 中国临床解剖学杂志, 2009, 27(5): 555557.
22.	张蕊, 马玉新, 刘艳婷. 超声对肠梗阻的诊断价值 [J]. 中外医疗, 2009, 28(4): 143.
23.	王志成, 王敏杰, 王纪龙. 肠梗阻的影像学诊断 [J]. 上海医学影像, 2009, 18(1): 4648.
24.	胡少辉, 沈雄山. 术后早期炎性肠梗阻诊治体会 [J]. 中国普外基础与临床杂志, 2010, 17(2): 184185.
25.	王静, 程桥. 长托宁与阿托品临床应用的效果观察 [J]. 山西医科大学学报, 2007, 38(3): 260261.

1. 罗东林, 张胜本, 刘宝华, 等. 老年急性肠梗阻的临床特点及其治疗(附150例报告) [J]. 中国普外基础与临床杂志, 2002, 9(6): 436437.
2. 梁庆丰, 刘海亮, 郭成方. 肠梗阻手术时机的临床分析 [J]. 医药论坛杂志, 2007, 28(5): 109110.
3. Foster NM, McGory ML, Zingmond DS, et al. Small bowel obstruction: a populationbased appraisal [J]. J Am Coll Surg, 2006, 203(2): 170176.
4. 陈孝平, 石应康, 邱贵兴, 等. 外科学 [M]. 北京: 人民卫生出版社, 2005: 588593.
5. 杨生虎, 高宁俊, 陈耀堂, 等. 肠梗阻的病因及诊治探讨 [J]. 现代医药卫生, 2008, 24(16): 23832385.
6. 刘建, 谷俊朝, 张忠涛, 等. 500 例肠梗阻病因分析及诊断探讨 [J]. 北京医学, 2006, 28(3): 162164.
7. Brandt RB, Siegel SA, Waters MG, et al. Spectrophotometric assay for D()lactate in plasma [J]. Anal Biochem, 1980, 102(1): 3946.
8. Phillips RK, Dudley HA. The effect of tetracycline lavage and trauma on visceral and parietal peritoneal ultrastructure and adhesion formation [J]. Br J Surg, 1984, 71(7): 537539.
9. Haglund U, Osterberg J. Local consequences of reperfusion in the gut//Grace PA, Mathie RT. IschemiaReperfusion Injury [M]. Oxford, UK: Blackwell Science, 1999: 6569.
10. 杨镇. 实验外科学 [M]. 上海: 上海科学技术出版社, 2004: 205.
11. 勒珠华, 井连平, 林秀珍, 等. 大承气汤对实验性不完全肠梗阻大鼠脾淋巴细胞内游离钙浓度的影响 [J]. 中国中西医结合杂志, 1997, 17(S): 150151.
12. Kawaguchi AL, Dunn JC, Lam M, et al. Glucose uptake in dilated small intestine [J]. J Pediatr Surg, 1998, 33(11): 16701673.
13. 陈明, 吴江, 袁汉尧. 不同类型肠梗阻血清中丙二醛的测定和细菌易位的实验研究 [J]. 广东医学院学报, 1999, 17(3): 201203.
14. 张志明, 张才全. 不同类型肠梗阻内毒素移位时段变化的实验研究 [J]. 中国普通外科杂志, 2007, 16(4): 389390.
15. 张英锋, 裴中会, 阮栋梁. SOD-一种超氧化物歧化酶 [J]. 化学世界, 2004, 45(2): 112.
16. 马可, 刘少华, 许兵, 等. 一氧化碳对内毒素血症肠道细胞凋亡的影响 [J]. 中华急诊医学杂志, 2006, 15(4): 323327.
17. 孙敬平, 马洪升. 应激状态下肠屏障功能的变化及其机理的研究进展 [J]. 中国普外基础与临床杂志, 2005, 12(3): 314316.
18. 杨牧祥, 于文涛, 徐华洲, 等. 中风康对局灶性脑缺血大鼠脑组织SOD MDA的影响 [J]. 中医药学刊, 2005, 23(8): 13651367.
19. 李宗辉. 藏雪莲水提取物对小白鼠组织内MDA含量的影响 [J]. 安徽农业科学, 2008, 36(35): 15510, 15534.
20. Smith SM, Eng RH, Buccini F. Use of Dlactic acid measurements in the diagnosis of bacterial infections [J]. J Infect Dis, 1986, 154(4): 658664.
21. 卢良声, 庄永敬, 吴桂荣, 等. 基于CT数据肠系膜上动脉三维重建及解剖学研究 [J]. 中国临床解剖学杂志, 2009, 27(5): 555557.
22. 张蕊, 马玉新, 刘艳婷. 超声对肠梗阻的诊断价值 [J]. 中外医疗, 2009, 28(4): 143.
23. 王志成, 王敏杰, 王纪龙. 肠梗阻的影像学诊断 [J]. 上海医学影像, 2009, 18(1): 4648.
24. 胡少辉, 沈雄山. 术后早期炎性肠梗阻诊治体会 [J]. 中国普外基础与临床杂志, 2010, 17(2): 184185.
25. 王静, 程桥. 长托宁与阿托品临床应用的效果观察 [J]. 山西医科大学学报, 2007, 38(3): 260261.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishing Rat Model of Incomplete Adhesive Intestinal Obstruction by Serosal Stripping Method

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