A Rat Model of Portal Hypertension with Hypersplenism_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHOU Ti , CHEN Yong , YOU Nan , DONG Xiaoping.

Department of Hepto-Biliary &;
Pancreato-Splenic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China;

Corresponding author：

Keywords：

Portal hypertension; Hypersplenism; Rat; Animal; Model

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo establish a model of portal hypertension with hypersplenism in SD rats by portal vein binding combined with splenic vein ligation. MethodsSixty healthy male SD rats were randomly divided into three groups: sham operation group （only laparotomy, n=20）, portal vein binding group (only binding, n=20), and portal vein binding combined with splenic vein ligation group (combined operation group, n=20). The counts of platelet, erythrocyte, and leukocyte were examined just before operation and once a week after operation for 7 weeks. Portal pressure, shortaxis, and longaxis diameter of spleen were examined just before operation and seven weeks after operation. At the seventh week, all the animals were sacrificed, spleen index and pathology changes of each group were examined. ResultsErythrocyte and platelet counts in combined operation group were significantly lower than those in the other two groups on the third week （P lt;0.05）, and there was no significant difference in leukocyte count among three groups （P gt;0.05）. Compared with the preoperative value, portal pressure increased significantly on the seventh week in both portal vein binding group and combined operation group, and was higher than that in the sham operation group （P lt;0.05）. The two diameters of spleen also increased significantly in combined operation group on the seventh week （P lt;0.05）, and were larger than those in the other two groups （P lt;0.05）. The same result was found in spleen index （P lt;0.05）. Typical pathological changes of hypersplenism presented only in combined operation group on the seventh week after operation. ConclusionsPortal vein binding combined with splenic vein ligation can induce experimental secondary hypersplenism successfully. This procedure is simple and stable, and helpful to the scientific research.

Citation： ZHOU Ti,CHEN Yong,YOU Nan,DONG Xiaoping.. A Rat Model of Portal Hypertension with Hypersplenism. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(6): 610-614. doi: Copy

1.	Musolino C, Calabrò L, Alonci A, et al. Hypersplenism: current status and perspectives [J]. Recenti Prog Med, 1999, 90(9): 488494.
2.	PeckRadosavljevic M. Hypersplenism [J]. Eur J Gastroenterol Hepatol, 2001, 13(4): 317323.
3.	夏穗生，曹秀峰，姜洪池. 现代脾脏外科学 [M]. 第2版. 南京: 江苏科学技术出版社, 2000: 163165．.
4.	陈维佩, 韩殿冰. 肝硬化门静脉高压症时脾功能与脾的病理生理 [J]. 中华肝胆外科杂志， 2006， 12(9)： 578579.
5.	王继见, 高根五. 门脉高压症发病机理的研究近况 [J]. 中国普外基础与临床杂志, 2000, 7(4): 275277.
6.	王继见, 高根五, 刘长安, 等. 用Wistar大鼠门静脉部分结扎制作门脉高压动物模型 [J]. 重庆医科大学学报, 2001, 26(3): 302304.
7.	徐义军，王超. 四氯化碳复合、梯段用药法制备大鼠门脉高压模型的研究 [J]. 中外医疗， 2010， 29(5): 12.
8.	周扬, 顾杰, 徐列明. 内皮素性大鼠门静脉高压模型的建立 [J]. 中国病理生理杂志， 2008， 24(4): 825827.
9.	Imura S, Shimada M, Utsunomiya T, et al. Impact of splenectomy in patients with liver cirrhosis: Results from 18 patients in a single center experience [J]. Hepatol Res, 2010, 40(9): 894900.
10.	Akahoshi T, Hashizume M, Tanoue K, et al. Role of the spleen in liver fibrosis in rats may be mediated by transforming growth factor beta1 [J]. J Gastroenterol Hepatol, 2002, 17(1): 5965.
11.	Murata K, Ito K, Yoneda K, et al. Splenectomy improves liver function in patients with liver cirrhosis [J]. Hepatogastroenterology, 2008, 55(85): 14071411.
12.	Bisharat N, Omari H, Lavi I, et al. Risk of infection and death among postsplenectomy patients [J]. J Infect, 2001, 43(3): 182186.
13.	Cadili A, de Gara C. Complications of splenectomy [J]. Am J Med, 2008, 121(5): 371375.
14.	Altman KI, Watman RN, Salomon K. Surgically induced splenogenic anaemia in the rabbit [J]. Nature, 1951, 168(4280): 827.
15.	Manning KL, McDonald TP. C3H mice have larger spleens, lower platelet counts, and shorter platelet lifespans than C57BL mice: an animal model for the study of hypersplenism [J]. Exp Hematol, 1997, 25(10): 10191024.
16.	刘经权，陈希炜，陈维佩，等. 脾肿大形态结构与吞噬功能关系的实验研究 [J]. 肝胆外科杂志， 1994， 2(1): 912．.
17.	Weissman SM, Waldmann TA, Levin E, et al. An attempt to produce hypersplenism in the dog, using methylcellulose [J]. Blood, 1961, 17: 632642.
18.	Zuckerman GB, Naughton BA, Gaito A, et al. The effect of methylcellulose on extrarenal erythropoietin production [J]. Proc Soc Exp Biol Med, 1984, 176(2): 197202.
19.	刘全达, 马宽生, 何振平, 等. 快速建立继发性脾功能亢进的犬动物模型 [J]. 第三军医大学学报, 2003, 25(4): 301304.
20.	王修忠，李恭才，朱壮涌. 脾功能亢进的实验研究及其临床应用 [J]. 中华小儿外科杂志， 1987, 8(2): 7172．.
21.	Sahin M, Tekin S, Aksoy F, et al. The effects of splenic artery ligation in an experimental model of secondary hypersplenism [J]. J R Coll Surg Edinb, 2000, 45(3): 148152.
22.	Salman H, Bessler H, Bergman M, et al. Functional hypersplenism in mice induced by adoptive transfer of syngeneic spleen cells [J]. Exp Biol Med (Maywood), 2006, 231(1): 112116.
23.	李宗芳, 刘保荣, 贾红生, 等. 大鼠门静脉主干缩窄后脾脏的变化研究 [J]. 中华实验外科杂志, 2004, 21(4): 433435.
24.	周丁华, 应大君, 肖凌云, 等. 大鼠肝硬变门脉高压症模型建立方法改进 [J]. 中国普外基础与临床杂志, 2000, 7(1): 2224.
25.	陈云志, 邓礼明, 廖毅, 等. 一种肝前性门静脉高压症模型的制备 [J]. 肝胆胰外科杂志, 2010, 22(1): 2831.
26.	李新秋, 吕云福. 肝硬化脾亢的动物模型与发病机制研究进展 [J]. 国际外科学杂志， 2007, 34(11): 775778.
27.	Groszmann RJ, Abraldes JG. Portal hypertension: from bedside to bench [J]. J Clin Gastroenterol, 2005, 39(4 Suppl 2): S125S130.

1. Musolino C, Calabrò L, Alonci A, et al. Hypersplenism: current status and perspectives [J]. Recenti Prog Med, 1999, 90(9): 488494.
2. PeckRadosavljevic M. Hypersplenism [J]. Eur J Gastroenterol Hepatol, 2001, 13(4): 317323.
3. 夏穗生，曹秀峰，姜洪池. 现代脾脏外科学 [M]. 第2版. 南京: 江苏科学技术出版社, 2000: 163165．.
4. 陈维佩, 韩殿冰. 肝硬化门静脉高压症时脾功能与脾的病理生理 [J]. 中华肝胆外科杂志， 2006， 12(9)： 578579.
5. 王继见, 高根五. 门脉高压症发病机理的研究近况 [J]. 中国普外基础与临床杂志, 2000, 7(4): 275277.
6. 王继见, 高根五, 刘长安, 等. 用Wistar大鼠门静脉部分结扎制作门脉高压动物模型 [J]. 重庆医科大学学报, 2001, 26(3): 302304.
7. 徐义军，王超. 四氯化碳复合、梯段用药法制备大鼠门脉高压模型的研究 [J]. 中外医疗， 2010， 29(5): 12.
8. 周扬, 顾杰, 徐列明. 内皮素性大鼠门静脉高压模型的建立 [J]. 中国病理生理杂志， 2008， 24(4): 825827.
9. Imura S, Shimada M, Utsunomiya T, et al. Impact of splenectomy in patients with liver cirrhosis: Results from 18 patients in a single center experience [J]. Hepatol Res, 2010, 40(9): 894900.
10. Akahoshi T, Hashizume M, Tanoue K, et al. Role of the spleen in liver fibrosis in rats may be mediated by transforming growth factor beta1 [J]. J Gastroenterol Hepatol, 2002, 17(1): 5965.
11. Murata K, Ito K, Yoneda K, et al. Splenectomy improves liver function in patients with liver cirrhosis [J]. Hepatogastroenterology, 2008, 55(85): 14071411.
12. Bisharat N, Omari H, Lavi I, et al. Risk of infection and death among postsplenectomy patients [J]. J Infect, 2001, 43(3): 182186.
13. Cadili A, de Gara C. Complications of splenectomy [J]. Am J Med, 2008, 121(5): 371375.
14. Altman KI, Watman RN, Salomon K. Surgically induced splenogenic anaemia in the rabbit [J]. Nature, 1951, 168(4280): 827.
15. Manning KL, McDonald TP. C3H mice have larger spleens, lower platelet counts, and shorter platelet lifespans than C57BL mice: an animal model for the study of hypersplenism [J]. Exp Hematol, 1997, 25(10): 10191024.
16. 刘经权，陈希炜，陈维佩，等. 脾肿大形态结构与吞噬功能关系的实验研究 [J]. 肝胆外科杂志， 1994， 2(1): 912．.
17. Weissman SM, Waldmann TA, Levin E, et al. An attempt to produce hypersplenism in the dog, using methylcellulose [J]. Blood, 1961, 17: 632642.
18. Zuckerman GB, Naughton BA, Gaito A, et al. The effect of methylcellulose on extrarenal erythropoietin production [J]. Proc Soc Exp Biol Med, 1984, 176(2): 197202.
19. 刘全达, 马宽生, 何振平, 等. 快速建立继发性脾功能亢进的犬动物模型 [J]. 第三军医大学学报, 2003, 25(4): 301304.
20. 王修忠，李恭才，朱壮涌. 脾功能亢进的实验研究及其临床应用 [J]. 中华小儿外科杂志， 1987, 8(2): 7172．.
21. Sahin M, Tekin S, Aksoy F, et al. The effects of splenic artery ligation in an experimental model of secondary hypersplenism [J]. J R Coll Surg Edinb, 2000, 45(3): 148152.
22. Salman H, Bessler H, Bergman M, et al. Functional hypersplenism in mice induced by adoptive transfer of syngeneic spleen cells [J]. Exp Biol Med (Maywood), 2006, 231(1): 112116.
23. 李宗芳, 刘保荣, 贾红生, 等. 大鼠门静脉主干缩窄后脾脏的变化研究 [J]. 中华实验外科杂志, 2004, 21(4): 433435.
24. 周丁华, 应大君, 肖凌云, 等. 大鼠肝硬变门脉高压症模型建立方法改进 [J]. 中国普外基础与临床杂志, 2000, 7(1): 2224.
25. 陈云志, 邓礼明, 廖毅, 等. 一种肝前性门静脉高压症模型的制备 [J]. 肝胆胰外科杂志, 2010, 22(1): 2831.
26. 李新秋, 吕云福. 肝硬化脾亢的动物模型与发病机制研究进展 [J]. 国际外科学杂志， 2007, 34(11): 775778.
27. Groszmann RJ, Abraldes JG. Portal hypertension: from bedside to bench [J]. J Clin Gastroenterol, 2005, 39(4 Suppl 2): S125S130.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

A Rat Model of Portal Hypertension with Hypersplenism

Abstract Full text Figures/Tables Video References Cited by

Format

Content