Effect of Sleeve Gastrectomy on LOX-1 Expression of Aortic Epithelium in Rats with High-Fat-Diet-Induced Obestiy_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Department of Biliary &;
Hernia &;
Bariatric Surgery, Shengjing Hospital, China Medical University， Shenyang 110004, Liaoning Province, China;

Corresponding author：

Keywords：

Sleeve gastrectomy; Morbid obesity; High fat-diet; Aorta

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo investigate the effect of sleeve gastrectomy on preventing adipose infiltration and LOX-1 expression in aortic epithelium of high-fat-diet-induced obese rats. MethodsTwenty-four Wistar rats were randomized into normal chow group (CO group), high-fat diet group (HD group), and high-fat diet combined with sleeve gastrectomy group (SG group). Rats of three groups before operation and CO group after operation were fed with normal diet, rats of HD and SG group were fed with high-fat diet. Body weight of all the rats were examined on day 10, 20, and 30 after operation, respectively. Animals were sacrificed on day 30 after operation and plasma HDL and LDL were detected by ELISA, LOX-1 and LOX-1 mRNA expression in aortic epithelium were measured by Western blot and real-time RT-PCR, respectively. Immunochemical histological Nile red stain was adopted in adipose infiltration examination of aorta. ResultsThe body weights in HD group were much higher than those of other groups （P lt;0.01）. The HDL in CO, HD, and SG groups was (32.9±6.2) mg/dl, (43.4± 4.0) mg/dl, and (37.5± 4.3) mg/dl, respectively. The LDL in CO, HD, and SG groups was (31.8±4.5) mg/dl, (53.3±5.1) mg/dl, and (40.5±3.7 ) mg/dl, respectively. The HDL and LDL values in HD group were higher than those of other two groups (P lt;0.05, P lt;0.01). The LOX-1 protein and mRNA expressions in HD group were much higher than those of other groups (P lt;0.01). Adipose staining in HD group was also ber than that of other groups. ConclusionsHigh-fat diet can induce elevation of LOX-1 protein and mRNA expression in aorta. Sleeve gastrecto my can relieve plasma LDL level, as a result, LOX-1 protein and mRNA expression should be down-regulated.

Citation： ZHOU Yong,WANG Yong,LIU Yuan,GENG Donghua,LIU Jingang.. Effect of Sleeve Gastrectomy on LOX-1 Expression of Aortic Epithelium in Rats with High-Fat-Diet-Induced Obestiy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(6): 615-619. doi: Copy

1.	Guijarro A, Kirchner H, Meguid MM. Catabolic effects of gastric bypass in a dietinduced obese rat model [J]. Curr Opin Clin Nutr Metab Care, 2006, 9(4): 423435.
2.	Zimmet P, Alberti KG, Shaw J. Global and societal implications of the diabetes epidemic [J]. Nature, 2001, 414(6865): 782787.
3.	Chen CM. Overview of obesity in Mainland China [J]. Obes Rev, 2008, 9 Suppl 1: 1421.
4.	Truesdale KP, Stevens J, Cai J. Effect of 3year weight history on blood pressure: the atherosclerosis risk in communities study [J]. Obesity (Silver Spring), 2008, 16(5): 11121119.
5.	Stevens VJ, Obarzanek E, Cook NR, et al. Longterm weight loss and changes in blood pressure: results of the Trials of Hypertension Prevention, phase Ⅱ [J]. Ann Intern Med, 2001, 134(1): 111.
6.	Dryvold WB, Midthjell K, Nilsen TI, et al. Change in body mass index and its impact on blood pressure: a prospective population study [J]. Int J Obes (Lond), 2005, 29(6): 6506557.
7.	Moore LL, Visioni AJ, Qureshi MM, et al. Weight loss in overweight adults and the longterm risk of hypertension: the Framingham study [J]. Arch Intern Med, 2005, 165(11): 12981303.
8.	Huang Z, Willett WC, Manson JE, et al. Body weight, weight change, and risk for hypertension in women [J]. Ann Intern Med, 1998, 128(2): 8188.
9.	National Institutes of Health. Clinical guidelines on the identification, evaluation, and treatment of overweight and obesity in adults-The evidence report [J]. Obes Res, 1998, 6 Suppl 2: 51S209S.
10.	Juhaeri, Stevens J, Chambless LE, et al. Associations between weight gain and incident hypertension in a biethnic cohort: the Atherosclerosis Risk in Communities Study [J]. Int J Obes Relat Metab Disord, 2002, 26(1): 5864.
11.	Field AE, Byers T, Hunter DJ, et al. Weight cycling, weight gain, and risk of hypertension in women [J]. Am J Epidemiol, 1999, 150(6): 573579.
12.	French SA, Jeffery RW, Folsom AR, et al. Weight loss maintenance in young adulthood: prevalence and correlations with health behavior and disease in a populationbased sample of women aged 5569 years [J]. Int J Obes Relat Metab Disord, 1996, 20(4): 303310.
13.	Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets [J]. J Cell Biol, 1985, 100(3): 965973.
14.	Visser M, Bouter LM, McQuillan GM, et al. Elevated Ceactive protein levels in overweight and obese adults [J]. JAMA, 1999, 282(22): 21312135.
15.	Ziccardi P, Nappo F, Giugliano G, et al. Reduction of inflammatory cytokine concentrations and improvement of endothelial functions in obese women after weight loss over one year [J]. Circulation, 2002, 105(7): 804809.
16.	Nigam A, Mitchell GF, Lambert J, et al. Relation between conduit vessel stiffness (assessed by tonometry) and endothelial function (assessed by flowmediated dilatation) in patients with and without coronary heart disease [J]. Am J Cardiol, 2003, 92(4): 395399.
17.	Shoelson SE, Herrero L, Naaz A. Obesity, inflammation, and insulin resistance [J]. Gastroenterology, 2007, 132(6): 21692180.
18.	Beach KW, Brunzell JD, Strandness DE Jr. Prevalence of severe arteriosclerosis obliterans in patients with diabetes mellitus. Relation to smoking and form of therapy [J]. Arteriosclerosis, 1982, 2(4): 275280.
19.	Sawamura T, Kume N, Aoyama T, et al. An endothelial receptor for oxidized lowdensity lipoprotein [J]. Nature, 1997, 386(6620): 7377.
20.	Moriwaki H, Kume N, Sawamura T, et al. Ligand specificity of LOX1, a novel endothelial receptor for oxidized low density lipoprotein [J]. Arterioscler Thromb Vasc Biol, 1998, 18(10): 15411547.
21.	Fraley AE, Tsimikas S. Clinical applications of circulating oxidized lowdensity lipoprotein biomarkers in cardiovascular disease [J]. Curr Opin Lipidol, 2006, 17(5): 502509.
22.	Matsuura E, Kobayashi K, Tabuchi M, et al. Oxidative modification of lowdensity lipoprotein and immune regulation of atherosclerosis [J]. Prog Lipid Res, 2006, 45(6): 466486.
23.	Chen J, Mehta JL, Haider N, et al. Role of caspases in OxLDLinduced apoptotic cascade in human coronary artery endothelial cells [J]. Circ Res, 2004, 94(3): 370376.
24.	Imanishi T, Hano T, Sawamura T, et al. Oxidized low density lipoprotein potentiation of Fasinduced apoptosis through lectinlike oxidizedlow density lipoprotein receptor1 in human umbilical vascular endothelial cells [J]. Circ J, 2002, 66(11): 10601064.
25.	Li DY, Zhang YC, Philips MI, et al. Upregulation of endothelial receptor for oxidized lowdensity lipoprotein (LOX1) in cultured human coronary artery endothelial cells by angiotensin Ⅱ type 1 receptor activation [J]. Circ Res, 1999, 84(9): 10431049.
26.	Kume N, Murase T, Moriwaki H, et al. Inducible expression of lectinlike oxidized LDL receptor1 in vascular endothelial cells [J]. Circ Res, 1998, 83(3): 322327.
27.	Murase T, Kume N, Korenaga R, et al. Fluid shear stress transcriptionally induces lectinlike oxidized LDL receptor1 in vascular endothelial cells [J]. Circ Res, 1998, 83(3): 328333.
28.	Chen M, Nagase M, Fujita T, et al. Diabetes enhances lectinlike oxidized LDL receptor1 (LOX1) expression in the vascular endothelium: possible role of LOX1 ligand and AGE [J]. Biochem Biophys Res Commun, 2001, 287(4): 962968.
29.	Steinbrook R. Surgery for severe obesity [J]. N Engl J Med, 2004, 350(11): 10751079.
30.	Pinkney JH, Sjstrm CD, Gale EA. Should surgeons treat diabetes in severely obese people? [J]. Lancet, 2001, 357(9265): 13571359.
31.	Dixon JB, Pories WJ, O’Brien PE, et al. Surgery as an effective early intervention for diabesity: why the reluctance? [J]. Diabetes Care, 2005, 28(2): 472474.
32.	Himpens J, Dapri G, Cadière GB. A prospective randomized study between laparoscopic gastric banding and laparoscopic isolated sleeve gastrectomy: results after 1 and 3 years [J]. Obes Surg, 2006, 16(11): 14501456.
33.	Moon Han S, Kim WW, Oh JH. Results of laparoscopic sleeve gastrectomy (LSG) at 1 year in morbidly obese Korean patients [J]. Obes Surg, 2005, 15(10): 14691475.
34.	Roa PE, KaidarPerson O, Pinto D, et al. Laparoscopic sleeve gastrectomy as treatment for morbid obesity: technique and shortterm outcome [J]. Obes Surg, 2006, 16(10): 13231326.
35.	Karamanakos SN, Vagenas K, Kalfarentzos F, et al. Weight loss, appetite suppression, and changes in fasting and postprandial ghrelin and peptideYY levels after RouxenY gastric bypass and sleeve gastrectomy: a prospective, double blind study [J]. Ann Surg, 2008, 247(3): 401407.

1. Guijarro A, Kirchner H, Meguid MM. Catabolic effects of gastric bypass in a dietinduced obese rat model [J]. Curr Opin Clin Nutr Metab Care, 2006, 9(4): 423435.
2. Zimmet P, Alberti KG, Shaw J. Global and societal implications of the diabetes epidemic [J]. Nature, 2001, 414(6865): 782787.
3. Chen CM. Overview of obesity in Mainland China [J]. Obes Rev, 2008, 9 Suppl 1: 1421.
4. Truesdale KP, Stevens J, Cai J. Effect of 3year weight history on blood pressure: the atherosclerosis risk in communities study [J]. Obesity (Silver Spring), 2008, 16(5): 11121119.
5. Stevens VJ, Obarzanek E, Cook NR, et al. Longterm weight loss and changes in blood pressure: results of the Trials of Hypertension Prevention, phase Ⅱ [J]. Ann Intern Med, 2001, 134(1): 111.
6. Dryvold WB, Midthjell K, Nilsen TI, et al. Change in body mass index and its impact on blood pressure: a prospective population study [J]. Int J Obes (Lond), 2005, 29(6): 6506557.
7. Moore LL, Visioni AJ, Qureshi MM, et al. Weight loss in overweight adults and the longterm risk of hypertension: the Framingham study [J]. Arch Intern Med, 2005, 165(11): 12981303.
8. Huang Z, Willett WC, Manson JE, et al. Body weight, weight change, and risk for hypertension in women [J]. Ann Intern Med, 1998, 128(2): 8188.
9. National Institutes of Health. Clinical guidelines on the identification, evaluation, and treatment of overweight and obesity in adults-The evidence report [J]. Obes Res, 1998, 6 Suppl 2: 51S209S.
10. Juhaeri, Stevens J, Chambless LE, et al. Associations between weight gain and incident hypertension in a biethnic cohort: the Atherosclerosis Risk in Communities Study [J]. Int J Obes Relat Metab Disord, 2002, 26(1): 5864.
11. Field AE, Byers T, Hunter DJ, et al. Weight cycling, weight gain, and risk of hypertension in women [J]. Am J Epidemiol, 1999, 150(6): 573579.
12. French SA, Jeffery RW, Folsom AR, et al. Weight loss maintenance in young adulthood: prevalence and correlations with health behavior and disease in a populationbased sample of women aged 5569 years [J]. Int J Obes Relat Metab Disord, 1996, 20(4): 303310.
13. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets [J]. J Cell Biol, 1985, 100(3): 965973.
14. Visser M, Bouter LM, McQuillan GM, et al. Elevated Ceactive protein levels in overweight and obese adults [J]. JAMA, 1999, 282(22): 21312135.
15. Ziccardi P, Nappo F, Giugliano G, et al. Reduction of inflammatory cytokine concentrations and improvement of endothelial functions in obese women after weight loss over one year [J]. Circulation, 2002, 105(7): 804809.
16. Nigam A, Mitchell GF, Lambert J, et al. Relation between conduit vessel stiffness (assessed by tonometry) and endothelial function (assessed by flowmediated dilatation) in patients with and without coronary heart disease [J]. Am J Cardiol, 2003, 92(4): 395399.
17. Shoelson SE, Herrero L, Naaz A. Obesity, inflammation, and insulin resistance [J]. Gastroenterology, 2007, 132(6): 21692180.
18. Beach KW, Brunzell JD, Strandness DE Jr. Prevalence of severe arteriosclerosis obliterans in patients with diabetes mellitus. Relation to smoking and form of therapy [J]. Arteriosclerosis, 1982, 2(4): 275280.
19. Sawamura T, Kume N, Aoyama T, et al. An endothelial receptor for oxidized lowdensity lipoprotein [J]. Nature, 1997, 386(6620): 7377.
20. Moriwaki H, Kume N, Sawamura T, et al. Ligand specificity of LOX1, a novel endothelial receptor for oxidized low density lipoprotein [J]. Arterioscler Thromb Vasc Biol, 1998, 18(10): 15411547.
21. Fraley AE, Tsimikas S. Clinical applications of circulating oxidized lowdensity lipoprotein biomarkers in cardiovascular disease [J]. Curr Opin Lipidol, 2006, 17(5): 502509.
22. Matsuura E, Kobayashi K, Tabuchi M, et al. Oxidative modification of lowdensity lipoprotein and immune regulation of atherosclerosis [J]. Prog Lipid Res, 2006, 45(6): 466486.
23. Chen J, Mehta JL, Haider N, et al. Role of caspases in OxLDLinduced apoptotic cascade in human coronary artery endothelial cells [J]. Circ Res, 2004, 94(3): 370376.
24. Imanishi T, Hano T, Sawamura T, et al. Oxidized low density lipoprotein potentiation of Fasinduced apoptosis through lectinlike oxidizedlow density lipoprotein receptor1 in human umbilical vascular endothelial cells [J]. Circ J, 2002, 66(11): 10601064.
25. Li DY, Zhang YC, Philips MI, et al. Upregulation of endothelial receptor for oxidized lowdensity lipoprotein (LOX1) in cultured human coronary artery endothelial cells by angiotensin Ⅱ type 1 receptor activation [J]. Circ Res, 1999, 84(9): 10431049.
26. Kume N, Murase T, Moriwaki H, et al. Inducible expression of lectinlike oxidized LDL receptor1 in vascular endothelial cells [J]. Circ Res, 1998, 83(3): 322327.
27. Murase T, Kume N, Korenaga R, et al. Fluid shear stress transcriptionally induces lectinlike oxidized LDL receptor1 in vascular endothelial cells [J]. Circ Res, 1998, 83(3): 328333.
28. Chen M, Nagase M, Fujita T, et al. Diabetes enhances lectinlike oxidized LDL receptor1 (LOX1) expression in the vascular endothelium: possible role of LOX1 ligand and AGE [J]. Biochem Biophys Res Commun, 2001, 287(4): 962968.
29. Steinbrook R. Surgery for severe obesity [J]. N Engl J Med, 2004, 350(11): 10751079.
30. Pinkney JH, Sjstrm CD, Gale EA. Should surgeons treat diabetes in severely obese people? [J]. Lancet, 2001, 357(9265): 13571359.
31. Dixon JB, Pories WJ, O’Brien PE, et al. Surgery as an effective early intervention for diabesity: why the reluctance? [J]. Diabetes Care, 2005, 28(2): 472474.
32. Himpens J, Dapri G, Cadière GB. A prospective randomized study between laparoscopic gastric banding and laparoscopic isolated sleeve gastrectomy: results after 1 and 3 years [J]. Obes Surg, 2006, 16(11): 14501456.
33. Moon Han S, Kim WW, Oh JH. Results of laparoscopic sleeve gastrectomy (LSG) at 1 year in morbidly obese Korean patients [J]. Obes Surg, 2005, 15(10): 14691475.
34. Roa PE, KaidarPerson O, Pinto D, et al. Laparoscopic sleeve gastrectomy as treatment for morbid obesity: technique and shortterm outcome [J]. Obes Surg, 2006, 16(10): 13231326.
35. Karamanakos SN, Vagenas K, Kalfarentzos F, et al. Weight loss, appetite suppression, and changes in fasting and postprandial ghrelin and peptideYY levels after RouxenY gastric bypass and sleeve gastrectomy: a prospective, double blind study [J]. Ann Surg, 2008, 247(3): 401407.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Sleeve Gastrectomy on LOX-1 Expression of Aortic Epithelium in Rats with High-Fat-Diet-Induced Obestiy

Abstract Full text Figures/Tables Video References Cited by

Format

Content