Expression of Chloride Intracellular Channel Protein 1 Protein Correlated with Tumorigenesis, Tumor Progress， and Prognosis of Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YANG Xiaosong , CHI Pan.

Department of General Surgery, Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China;

Corresponding author：

Keywords：

Colorectal cancer; Chloride intracellular channel protein 1; Immunohistochemistry; Tissue microarray; Survival analysis

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo explore the expression of chloride intracellular channel protein 1 (CLIC1) protein in the matched colorectal normal mucosa tissue, colorectal adenoma tissue， and colorectal cancer tissue, and its relationship with tumorigenesis, tumor progression， and prognosis of patients with colorectal cancer . MethodsThe expression of CLIC1 protein was detected in 150 cases of colorectal normal mucosa tissues, 62 cases of colorectal adenoma tissues, and 187 cases of colorectal cancer tissues by using immunohistochemistry tissue microarray, and the relationships between the expression of CLIC1 protein and clinicopathologic features, and the survival rate of patients with colorectal cancer were analyzed. ResultsThe positive rate of CLIC1 protein expression in normal mucosa tissues (26.00%, 39/150), colorectal adenoma tissues (66.13%, 41/62), and colorectal cancer tissues (82.89%, 187/155) increased in turn and the difference was statistically significant (P lt;0.001). The expression of CLIC1 protein was related to TNM staging (P=0.007), but it was not related to gender (P=0.553), age (P=0.206), tumor diameter (P=0.185), tumor differentiation (P=0.062), and tumor location (P=0.598). The median survival time after surgery in patients with CLIC1 protein positive expression was 80 months, and it was 111 months in patients with CLIC1 protein negative expression. The survival rate of patients with CLIC1 protein positive expression was lower than that with CLIC1 protein negative expression by log-rank test (66.40% vs. 80.00%, P=0.031). ConclusionsThe expression of CLIC1 protein is related to the tumorigenesis and progression of colorectal cancer as well as the survival of patients with colorectal cancer. CLIC1 is a potential tumor biomarker.

Citation： YANG Xiaosong,CHI Pan.. Expression of Chloride Intracellular Channel Protein 1 Protein Correlated with Tumorigenesis, Tumor Progress， and Prognosis of Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(7): 745-749. doi: Copy

1.	Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2009 [J]. CA Cancer J Clin, 2009, 59(4): 225249.
2.	Waliszewski P. Controversies about the genetic model of colorectal tumorigenesis [J]. Pol J Pathol, 1995, 46(4): 239243.
3.	Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis [J]. Cell, 1990, 61(5): 759767.
4.	Ilyas M, Straub J, Tomlinson IP, et al. Genetic pathways in colorectal and other cancers [J]. Eur J Cancer, 1999, 35(14): 19862002.
5.	Ulmasov B, Bruno J, Woost PG, et al. Tissue and subcellular distribution of CLIC1 [J]. BMC Cell Biol, 2007, 8: 8.
6.	Chang YH, Wu CC, Chang KP, et al. Cell secretome analysis using hollow fiber culture system leads to the discovery of CLIC1 protein as a novel plasma marker for nasopharyngeal carcinoma [J]. J Proteome Res, 2009, 8(12): 54655474.
7.	Chen CD, Wang CS, Huang YH, et al. Overexpression of CLIC1 in human gastric carcinoma and its clinicopathological significance [J]. Proteomics, 2007, 7(1): 155167.
8.	Wang JW, Peng SY, Li JT, et al. Identification of metastasisassociated proteins involved in gallbladder carcinoma metastasis by proteomic analysis and functional exploration of chloride intracellular channel 1 [J]. Cancer Lett, 2009, 281(1): 7181.
9.	Petrova DT, Asif AR, Armstrong VW, et al. Expression of chloride intracellular channel protein 1 (CLIC1) and tumor protein D52 (TPD52) as potential biomarkers for colorectal cancer [J]. Clin Biochem, 2008, 41(1415): 12241236.
10.	李荣宽, 唐建武, 张军, 等. 胞内氯离子通道蛋白1基因沉默对小鼠肝癌细胞株增殖及侵袭能力的影响 [J]. 中华肝脏病杂志, 2010, 18(2): 131135.
11.	Edge SB, Byrd DR, Compton CC, et al. eds. AJCC Cancer Staging Manual [M]. 7th ed. New York, Springer, 2010: 143164.
12.	Luo J, Zha S, Gage WR, et al. AlphamethylacylCoA racemase: a new molecular marker for prostate cancer [J]. Cancer Res, 2002, 62(8): 22202226.
13.	Qiu MR, Jiang L, Matthaei KI, et al. Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene [J]. Genesis, 2010, 48(2): 127136.
14.	Berry KL, Hobert O. Mapping functional domains of chloride intracellular channel (CLIC) proteins in vivo [J]. J Mol Biol, 2006, 359(5): 13161333.
15.	Stoychev SH, Nathaniel C, Fanucchi S, et al. Structural dynamics of soluble chloride intracellular channel protein CLIC1 examined by amide hydrogendeuterium exchange mass spectrometry [J]. Biochemistry, 2009, 48(35): 84138421.
16.	Littler DR, Harrop SJ, Fairlie WD, et al. The intracellular chloride ion channel protein CLIC1 undergoes a redoxcontrolled structural transition [J]. J Biol Chem, 2004, 279(10): 92989305.
17.	Kim JS, Chang JW, Yun HS, et al. Chloride intracellular channel 1 identified using proteomic analysis plays an important role in the radiosensitivity of HEp2 cells via reactive oxygen species production [J]. Proteomics, 2010, 10(14): 25892604.
18.	Goodchild SC, Howell MW, Cordina NM, et al. Oxidation promotes insertion of the CLIC1 chloride intracellular channel into the membrane [J]. Eur Biophys J, 2009, 39(1): 129138.
19.	Singh H, Ashley RH. Redox regulation of CLIC1 by cysteine residues associated with the putative channel pore [J]. Biophys J, 2006, 90(5): 16281638.
20.	Averaimo S, Milton RH, Duchen MR, et al. Chloride intracellular channel 1 (CLIC1): sensor and effector during oxidative stress [J]. FEBS Lett, 2010, 584(10): 20762084.
21.	Behrend L, Henderson G, Zwacka RM. Reactive oxygen species in oncogenic transformation [J]. Biochem Soc Trans, 2003, 31(Pt 6): 14411444.
22.	Wu WS. The signaling mechanism of ROS in tumor progression [J]. Cancer Metastasis Rev, 2006, 25(4): 695705.
23.	Valenzuela SM, Mazzanti M, Tonini R, et al. The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle [J]. J Physiol, 2000, 529(Pt 3): 541552.
24.	Sun HJ, Bahk YY, Choi YR, et al. A proteomic analysis during serial subculture and osteogenic differentiation of human mesenchymal stem cell [J]. J Orthop Res, 2006, 24(11): 20592071.
25.	Menon SG, Goswami PC. A redox cycle within the cell cycle: ring in the old with the new [J]. Oncogene, 2007, 26(8): 11011109.
26.	Paradisi S, Matteucci A, Fabrizi C, et al. Blockade of chloride intracellular ion channel 1 stimulates Abeta phagocytosis [J]. J Neurosci Res, 2008, 86(11): 24882498.

1. Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2009 [J]. CA Cancer J Clin, 2009, 59(4): 225249.
2. Waliszewski P. Controversies about the genetic model of colorectal tumorigenesis [J]. Pol J Pathol, 1995, 46(4): 239243.
3. Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis [J]. Cell, 1990, 61(5): 759767.
4. Ilyas M, Straub J, Tomlinson IP, et al. Genetic pathways in colorectal and other cancers [J]. Eur J Cancer, 1999, 35(14): 19862002.
5. Ulmasov B, Bruno J, Woost PG, et al. Tissue and subcellular distribution of CLIC1 [J]. BMC Cell Biol, 2007, 8: 8.
6. Chang YH, Wu CC, Chang KP, et al. Cell secretome analysis using hollow fiber culture system leads to the discovery of CLIC1 protein as a novel plasma marker for nasopharyngeal carcinoma [J]. J Proteome Res, 2009, 8(12): 54655474.
7. Chen CD, Wang CS, Huang YH, et al. Overexpression of CLIC1 in human gastric carcinoma and its clinicopathological significance [J]. Proteomics, 2007, 7(1): 155167.
8. Wang JW, Peng SY, Li JT, et al. Identification of metastasisassociated proteins involved in gallbladder carcinoma metastasis by proteomic analysis and functional exploration of chloride intracellular channel 1 [J]. Cancer Lett, 2009, 281(1): 7181.
9. Petrova DT, Asif AR, Armstrong VW, et al. Expression of chloride intracellular channel protein 1 (CLIC1) and tumor protein D52 (TPD52) as potential biomarkers for colorectal cancer [J]. Clin Biochem, 2008, 41(1415): 12241236.
10. 李荣宽, 唐建武, 张军, 等. 胞内氯离子通道蛋白1基因沉默对小鼠肝癌细胞株增殖及侵袭能力的影响 [J]. 中华肝脏病杂志, 2010, 18(2): 131135.
11. Edge SB, Byrd DR, Compton CC, et al. eds. AJCC Cancer Staging Manual [M]. 7th ed. New York, Springer, 2010: 143164.
12. Luo J, Zha S, Gage WR, et al. AlphamethylacylCoA racemase: a new molecular marker for prostate cancer [J]. Cancer Res, 2002, 62(8): 22202226.
13. Qiu MR, Jiang L, Matthaei KI, et al. Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene [J]. Genesis, 2010, 48(2): 127136.
14. Berry KL, Hobert O. Mapping functional domains of chloride intracellular channel (CLIC) proteins in vivo [J]. J Mol Biol, 2006, 359(5): 13161333.
15. Stoychev SH, Nathaniel C, Fanucchi S, et al. Structural dynamics of soluble chloride intracellular channel protein CLIC1 examined by amide hydrogendeuterium exchange mass spectrometry [J]. Biochemistry, 2009, 48(35): 84138421.
16. Littler DR, Harrop SJ, Fairlie WD, et al. The intracellular chloride ion channel protein CLIC1 undergoes a redoxcontrolled structural transition [J]. J Biol Chem, 2004, 279(10): 92989305.
17. Kim JS, Chang JW, Yun HS, et al. Chloride intracellular channel 1 identified using proteomic analysis plays an important role in the radiosensitivity of HEp2 cells via reactive oxygen species production [J]. Proteomics, 2010, 10(14): 25892604.
18. Goodchild SC, Howell MW, Cordina NM, et al. Oxidation promotes insertion of the CLIC1 chloride intracellular channel into the membrane [J]. Eur Biophys J, 2009, 39(1): 129138.
19. Singh H, Ashley RH. Redox regulation of CLIC1 by cysteine residues associated with the putative channel pore [J]. Biophys J, 2006, 90(5): 16281638.
20. Averaimo S, Milton RH, Duchen MR, et al. Chloride intracellular channel 1 (CLIC1): sensor and effector during oxidative stress [J]. FEBS Lett, 2010, 584(10): 20762084.
21. Behrend L, Henderson G, Zwacka RM. Reactive oxygen species in oncogenic transformation [J]. Biochem Soc Trans, 2003, 31(Pt 6): 14411444.
22. Wu WS. The signaling mechanism of ROS in tumor progression [J]. Cancer Metastasis Rev, 2006, 25(4): 695705.
23. Valenzuela SM, Mazzanti M, Tonini R, et al. The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle [J]. J Physiol, 2000, 529(Pt 3): 541552.
24. Sun HJ, Bahk YY, Choi YR, et al. A proteomic analysis during serial subculture and osteogenic differentiation of human mesenchymal stem cell [J]. J Orthop Res, 2006, 24(11): 20592071.
25. Menon SG, Goswami PC. A redox cycle within the cell cycle: ring in the old with the new [J]. Oncogene, 2007, 26(8): 11011109.
26. Paradisi S, Matteucci A, Fabrizi C, et al. Blockade of chloride intracellular ion channel 1 stimulates Abeta phagocytosis [J]. J Neurosci Res, 2008, 86(11): 24882498.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of Chloride Intracellular Channel Protein 1 Protein Correlated with Tumorigenesis, Tumor Progress， and Prognosis of Colorectal Cancer

Abstract Full text Figures/Tables Video References Cited by

Format

Content